Effects of noise and linewidth on in vivo analysis of glutamate at 3 T.

Magnetic resonance spectroscopy (MRS) can noninvasively detect metabolites in vivo, including glutamate (Glu). However, quantification is known to be affected by the overlaps among metabolite resonance lines and background macromolecule signals. We found that adding a moderate amount of noise or line broadening (2 Hz) caused large variations in concentration of Glu and other metabolites, when determined by LCModel analysis of in vivo short-echo time (TE) spectra. Theses variations were largely attributed to strong spectral baselines in short TE spectra, especially near 2.35 ppm, as well as overlapping metabolite resonance lines. To address this issue, we acquired in vivo data at 3 T using both short-TE and the multiple echo time J-resolved point-resolved spectroscopy (JPRESS) MRS techniques. We found that one-dimensional (1D) JPRESS, by simultaneously fitting the two cross-sections of JPRESS at J = 0 and J = 7.5 Hz, was highly resistant to variations in noise levels and spectral linewidths. Our results demonstrate that LCModel analysis of short-TE data is highly sensitive to variations in noise levels and spectral linewidths and this sensitivity is greatly reduced by 1D JPRESS given its substantially reduced baselines and enhanced spectral resolution.