Literature DB >> 32360381

New primers, taxonomic database and cut-off value for processing nxrB gene high-throughput sequencing data by MOTHUR.

Yiguo Hong1, Lijing Jiao2, Jiapeng Wu2.   

Abstract

To analyse the diversity of Nitrospira with functional nxrB genes, we designed and optimized a new primer set, Nxr-f27/Nxr-r617, and constructed a database for taxonomic classification. We also propose a cut-off value for processing the high-throughput sequencing data with MOTHUR.
Copyright © 2020 Elsevier B.V. All rights reserved.

Keywords:  Cut-off; Nitrospira; Primer; Taxonomic database; nxrB gene

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Year:  2020        PMID: 32360381     DOI: 10.1016/j.mimet.2020.105939

Source DB:  PubMed          Journal:  J Microbiol Methods        ISSN: 0167-7012            Impact factor:   2.363


  2 in total

1.  Evaluation of PCR primers for detecting the distribution of nitrifiers in mangrove sediments.

Authors:  Shanshan Meng; Tao Peng; Hui Wang; Tongwang Huang; Ji-Dong Gu; Zhong Hu
Journal:  Appl Microbiol Biotechnol       Date:  2022-08-09       Impact factor: 5.560

2.  Existence and distribution of novel phylotypes of Nitrospira in water columnsof the South China Sea.

Authors:  Wei Sun; Lijing Jiao; Jiapeng Wu; Jiaqi Ye; Mingken Wei; Yiguo Hong
Journal:  iScience       Date:  2022-08-08
  2 in total

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