| Literature DB >> 32359951 |
Gazi Sakir Hossain1, Mukesh Saini1, Ryoma Miyake2, Hua Ling1, Matthew Wook Chang3.
Abstract
Low yield and low titer of natural products are common issues in natural product biosynthesis through microbial cell factories. One effective way to resolve such bottlenecks is to design genetic biosensors to monitor and regulate the biosynthesis of target natural products. In this review, we evaluate the most recent advances in the design of genetic biosensors for natural product biosynthesis in microorganisms. In particular, we examine strategies for selection of genetic parts and construction principles for the design and evaluation of genetic biosensors. We also review the latest applications of transcription factor- and riboswitch-based genetic biosensors in natural product biosynthesis. Lastly, we discuss challenges and solutions in designing genetic biosensors for the biosynthesis of natural products in microorganisms.Keywords: biosynthetic pathway; genetic biosensor; natural product; riboswitch; transcription factor
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Year: 2020 PMID： 32359951 DOI： 10.1016/j.tibtech.2020.03.013
Source DB: PubMed Journal: Trends Biotechnol ISSN： 0167-7799 Impact factor: 19.536