| Literature DB >> 3235771 |
B Fudem-Goldin1, P Voulalas, G A Orr.
Abstract
UDP[6-3H]galactose of high specificity can be obtained by oxidation of the C-6 hydroxymethyl group of UDP-galactose by galactose oxidase and subsequent reduction by sodium borotritide. One-step purification of the nucleotide sugar involves anion-exchange chromatography on a Pharmacia Mono Q column. Radiolabeled UDP-N-acetylgalactosamine can also be synthesized and purified by this procedure. Both nucleotide sugars can be used for sugar incorporation studies using the appropriate glycosyltransferase.Entities:
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Year: 1988 PMID： 3235771 DOI： 10.1016/0165-022x(88)90004-8
Source DB: PubMed Journal: J Biochem Biophys Methods ISSN： 0165-022X