Literature DB >> 3235763

A convenient spectroscopic method for the estimation of hemoglobin concentrations in cell-free solutions.

S M Snell1, M A Marini.   

Abstract

Using a millimolar absorptivity of 7.12 +/- 0.09 at 523 nm, it is possible to estimate the total concentration of hemoglobin in solutions containing oxyhemoglobin, deoxyhemoglobin and methemoglobin in any combination. This estimate is independent of the pH in the range 6.0-10.0 and will provide concentrations comparable to that obtained by the conventional and more precise use of cyanomethemoglobin. This methodology should be of value for the determination of extracellular hemoglobin in vivo, as a means for determining the vascular half-life of stroma-free hemoglobin based blood substitutes.

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Year:  1988        PMID： 3235763     DOI： 10.1016/0165-022x(88)90075-9

Source DB:  PubMed          Journal:  J Biochem Biophys Methods        ISSN： 0165-022X


  9 in total

1.  Oxidation and haem loss kinetics of poly(ethylene glycol)-conjugated haemoglobin (MP4): dissociation between in vitro and in vivo oxidation rates.

Authors:  Kim D Vandegriff; Ashok Malavalli; Charles Minn; Eva Jiang; Jeff Lohman; Mark A Young; Michele Samaja; Robert M Winslow
Journal:  Biochem J       Date:  2006-11-01       Impact factor: 3.857

2.  Soft X-ray microscopy analysis of cell volume and hemoglobin content in erythrocytes infected with asexual and sexual stages of Plasmodium falciparum.

Authors:  Eric Hanssen; Christian Knoechel; Megan Dearnley; Matthew W A Dixon; Mark Le Gros; Carolyn Larabell; Leann Tilley
Journal:  J Struct Biol       Date:  2011-09-16       Impact factor: 2.867

3.  CO-MP4, a polyethylene glycol-conjugated haemoglobin derivative and carbon monoxide carrier that reduces myocardial infarct size in rats.

Authors:  K D Vandegriff; M A Young; J Lohman; A Bellelli; M Samaja; A Malavalli; R M Winslow
Journal:  Br J Pharmacol       Date:  2008-06-09       Impact factor: 8.739

4.  Kinetics of NO and O2 binding to a maleimide poly(ethylene glycol)-conjugated human haemoglobin.

Authors:  Kim D Vandegriff; Andrea Bellelli; Michele Samaja; Ashok Malavalli; Maurizio Brunori; Robert M Winslow
Journal:  Biochem J       Date:  2004-08-15       Impact factor: 3.857

5.  Hemoglobin-associated oxidative stress in the pericardial compartment of postoperative cardiac surgery patients.

Authors:  Philip A Kramer; Balu K Chacko; Saranya Ravi; Michelle S Johnson; Tanecia Mitchell; Stephen Barnes; Alireza Arabshahi; Louis J Dell'Italia; David J George; Chad Steele; James F George; Victor M Darley-Usmar; Spencer J Melby
Journal:  Lab Invest       Date:  2014-12-01       Impact factor: 5.662

6.  Dissection of the radical reactions linked to fetal hemoglobin reveals enhanced pseudoperoxidase activity.

Authors:  Khuanpiroon Ratanasopa; Michael Brad Strader; Abdu I Alayash; Leif Bulow
Journal:  Front Physiol       Date:  2015-02-20       Impact factor: 4.566

7.  Hemolytic and antimalarial effects of tight-binding glyoxalase 1 inhibitors on the host-parasite unit of erythrocytes infected with Plasmodium falciparum.

Authors:  Cletus A Wezena; Miriam Urscher; Robert Vince; Swati S More; Marcel Deponte
Journal:  Redox Biol       Date:  2016-03-02       Impact factor: 11.799

8.  Scavenging of nitric oxide by hemoglobin in the tunica media of porcine coronary arteries.

Authors:  Alexander Kollau; Michael Russwurm; Andrea Neubauer; Gerald Rechberger; Kurt Schmidt; Doris Koesling; John Fassett; Astrid Schrammel; Bernd Mayer
Journal:  Nitric Oxide       Date:  2016-01-22       Impact factor: 4.427

9.  The malaria parasite sheddase SUB2 governs host red blood cell membrane sealing at invasion.

Authors:  Christine R Collins; Fiona Hackett; Steven A Howell; Ambrosius P Snijders; Matthew Rg Russell; Lucy M Collinson; Michael J Blackman
Journal:  Elife       Date:  2020-12-08       Impact factor: 8.713
  9 in total

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