Maryam Zadsar1, Mohammad Reza Shirzadi2, Mohammad Zeynali2, Mahboubeh Rasouli3, Gharib Karimi1. 1. Blood Transfusion Research Centre, High Institute for Research and Education in Transfusion Medicine, Tehran, Iran. 2. Centre for Disease Control, Ministry of Health, Tehran, Iran. 3. Department of Biostatics, School of Public Health, Iran University of Medical Sciences, Tehran, Iran.
Abstract
BACKGROUND: Brucellosis is a common zoonotic infection worldwide. Transmission can be occasionally observed via transfusion or transplantation. This study was designed to survey the seroprevalence of anti-Brucella antibody in blood donors in different endemic provinces. MATERIALS AND METHODS: A total of 14,706 blood donors from the 5 most prevalent provinces were studied by standard tube agglutination (STA) and any positive subjects were further confirmed by 2-mercaptoethanol agglutination test (2-ME). Significant titres were 80 for STA and 40 for 2-ME. RESULT: A total of 63 (0.43%) serum samples were STA-positive. Of these, the 2-ME test was reactive in 42 samples. The 2-ME test was reactive in 31 samples with a low titre. However, concomitant STA ≥80 and the titre of ≥40 for the 2-ME test were found in 11 subjects (0.075%), mostly resident in urban areas. Exposure to manure products was identified as a significant risk factor (p = 0.0128). Conclusion : The observed data show a somewhat noticeable prevalence among Iranian blood donors, bringing attention to the importance of pre-donation screening via a questionnaire which supplies answers about occupational history, and any history of exposure or past infections. Further studies to evaluate the frequency and related risk factors in certain populations, in conjunction with implementing stricter regulations in blood donor selection in endemic areas, may be necessary.
BACKGROUND: Brucellosis is a common zoonotic infection worldwide. Transmission can be occasionally observed via transfusion or transplantation. This study was designed to survey the seroprevalence of anti-Brucella antibody in blood donors in different endemic provinces. MATERIALS AND METHODS: A total of 14,706 blood donors from the 5 most prevalent provinces were studied by standard tube agglutination (STA) and any positive subjects were further confirmed by 2-mercaptoethanol agglutination test (2-ME). Significant titres were 80 for STA and 40 for 2-ME. RESULT: A total of 63 (0.43%) serum samples were STA-positive. Of these, the 2-ME test was reactive in 42 samples. The 2-ME test was reactive in 31 samples with a low titre. However, concomitant STA ≥80 and the titre of ≥40 for the 2-ME test were found in 11 subjects (0.075%), mostly resident in urban areas. Exposure to manure products was identified as a significant risk factor (p = 0.0128). Conclusion : The observed data show a somewhat noticeable prevalence among Iranian blood donors, bringing attention to the importance of pre-donation screening via a questionnaire which supplies answers about occupational history, and any history of exposure or past infections. Further studies to evaluate the frequency and related risk factors in certain populations, in conjunction with implementing stricter regulations in blood donor selection in endemic areas, may be necessary.
Authors: Jacques Godfroid; Axel Cloeckaert; Jean-Pierre Liautard; Stephan Kohler; David Fretin; Karl Walravens; Bruno Garin-Bastuji; Jean-Jacques Letesson Journal: Vet Res Date: 2005 May-Jun Impact factor: 3.683
Authors: Fariba Keramat; Mohammad Mehdi Majzobi; Jalal Poorolajal; Zohreh Zarei Ghane; Maryam Adabi Journal: Osong Public Health Res Perspect Date: 2017-08-31