Hyebin Koh1, Hu-Nan Sun2, Zhen Xing3, Ren Liu3, Nisansala Chandimali3, Taeho Kwon4, Dong-Sun Lee5,6,7,8. 1. Department of Animal Biotechnology, Jeju National University, Jeju, Republic of Korea. 2. College of Life Science and Biotechnology, Heilongjiang Bayi Agricultural University, Daqing, P.R. China. 3. Interdisciplinary Graduate Program in Advanced Convergence Technology and Science, Jeju National University, Jeju, Republic of Korea. 4. Primate Resources Center, Korea Research Institute of Bioscience and Biotechnology, Jeonbuk, Republic of Korea. 5. Interdisciplinary Graduate Program in Advanced Convergence Technology and Science, Jeju National University, Jeju, Republic of Korea dongsunlee@jejunu.ac.kr. 6. Subtropical/Tropical Organism Gene Bank, Jeju National University, Jeju, Republic of Korea. 7. Faculty of Biotechnology, College of Applied Life Sciences, Jeju National University, Jeju, Republic of Korea. 8. Practical Translational Research Center, Jeju National University, Jeju, Republic of Korea.
Abstract
Backgorund/Aim: Wogonin, a flavonoid-like compound extracted from the root of Scutellaria baicalensis Georgi, has been shown to have anticancer effects against cancer cells. Osteosarcoma is the most malignant type of bone cancer and can appear in any bone, with a high propensity for relapse and metastasis. The present study aimed to assess the anticancer effects of wogonin on osteosarcoma stem cells. MATERIALS AND METHODS: The cytotoxic effects of wogonin on CD133+ Cal72 osteosarcoma stem cells were assessed through in vitro experiments by MTT assay, transwell assay, sphere-formation assay, flow cytometry, immunocytochemistry and western blotting. RESULTS: Wogonin suppressed stem cell characteristics and the expression of stem cell-related genes by regulating reactive oxygen species (ROS) levels and ROS-related signaling of CD133+ Cal72 cells, effects which were reversed by ROS scavenger N-acetylcysteine. CONCLUSION: Wogonin may be a promising candidate for successful clinical management of osteosarcoma by regulating ROS-related mechanisms and stem cell-related genes. Copyright
Backgorund/Aim: Wogonin, a flavonoid-like compound extracted from the root of Scutellaria baicalensis Georgi, has been shown to have anticancer effects against cancer cells. Osteosarcoma is the most malignant type of bone cancer and can appear in any bone, with a high propensity for relapse and metastasis. The present study aimed to assess the anticancer effects of wogonin on osteosarcoma stem cells. MATERIALS AND METHODS: The cytotoxic effects of wogonin on CD133+ Cal72 osteosarcoma stem cells were assessed through in vitro experiments by MTT assay, transwell assay, sphere-formation assay, flow cytometry, immunocytochemistry and western blotting. RESULTS:Wogonin suppressed stem cell characteristics and the expression of stem cell-related genes by regulating reactive oxygen species (ROS) levels and ROS-related signaling of CD133+ Cal72 cells, effects which were reversed by ROS scavenger N-acetylcysteine. CONCLUSION:Wogonin may be a promising candidate for successful clinical management of osteosarcoma by regulating ROS-related mechanisms and stem cell-related genes. Copyright
Authors: Ander Abarrategi; Juan Tornin; Lucia Martinez-Cruzado; Ashley Hamilton; Enrique Martinez-Campos; Juan P Rodrigo; M Victoria González; Nicola Baldini; Javier Garcia-Castro; Rene Rodriguez Journal: Stem Cells Int Date: 2016-06-05 Impact factor: 5.443