Núria Nieto-Nicolau1, Beatriz Martín-Antonio2, Claudia Müller-Sánchez3, Ricardo P Casaroli-Marano1,4,5. 1. Barcelona Tissue Bank, Banc de Sang I Teixits (BST), Barcelona, Spain. 2. Josep Carreras Leukaemia Research Institute (IDIBAPS), Barcelona, Spain. 3. Department of Cell Biology, CellTec-UB, University of Barcelona, Barcelona, Spain. 4. Department of Surgery, School of Medicine & Hospital Clinic de Barcelona, University of Barcelona, Barcelona, Spain. 5. Institute of Biomedical Research Sant Pau (IIB-Sant Pau), Barcelona, Spain.
Abstract
Aim: To determine the potential of mesenchymal stem cells (MSC) for corneal epithelial regeneration in vitro. Materials & methods: Bone marrow MSC (BM-MSC) and adipose tissue MSC were analyzed for corneal epithelial and mesenchymal markers, using limbal stem cells and corneal cells as controls. MSC with better potential were cultured with specific mediums for epithelial induction. Transepithelial electric resistance and wound healing assay with human corneal epithelial cells were performed. Results: BM-MSC showed better potential, increased corneal markers, and higher transepithelial electric resistance values when induced with limbal epithelial culture medium. Induced BM-MSC promoted better wound healing of human corneal epithelial cells by paracrine secretion. Conclusion: BM-MSC has potential for corneal epithelial induction in a protocol compatible with human application.
Aim: To determine the potential of mesenchymal stem cells (MSC) for corneal epithelial regeneration in vitro. Materials & methods: Bone marrow MSC (BM-MSC) and adipose tissue MSC were analyzed for corneal epithelial and mesenchymal markers, using limbal stem cells and corneal cells as controls. MSC with better potential were cultured with specific mediums for epithelial induction. Transepithelial electric resistance and wound healing assay with human corneal epithelial cells were performed. Results: BM-MSC showed better potential, increased corneal markers, and higher transepithelial electric resistance values when induced with limbal epithelial culture medium. Induced BM-MSC promoted better wound healing of human corneal epithelial cells by paracrine secretion. Conclusion: BM-MSC has potential for corneal epithelial induction in a protocol compatible with human application.
Authors: Dawidson Assis Gomes; Alfredo Miranda de Goes; Thaís Maria da Mata Martins; Juliana Lott de Carvalho; Pricila da Silva Cunha Journal: Stem Cell Rev Rep Date: 2022-03-05 Impact factor: 6.692