Literature DB >> 32352044

Corrigendum.

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Abstract

[This corrects the article DOI: 10.1002/pld3.160.].
© 2020 The Authors. Plant Direct published by American Society of Plant Biologists, Society for Experimental Biology and John Wiley & Sons Ltd.

Entities:  

Year:  2020        PMID: 32352044      PMCID: PMC7187629          DOI: 10.1002/pld3.222

Source DB:  PubMed          Journal:  Plant Direct        ISSN: 2475-4455


In the article by Sakata et al., in the section “2.3 Plasmid construction” in the EXPERIMENTAL PROCEDURES, the author indicated the wrong plasmid (lines 9–10) and failed to describe the procedure of the plasmids (lines 10–15). The correct text should read as follows: “2.3 Plasmid construction” The plasmids used in this study were constructed with the Gateway cloning technology (Invitrogen). To construct Mpphot‐Citrine, MpPHOT was transferred from the pDONR207‐MpPHOT plasmid (Kodama, 2016) to the pMpGWB306 plasmid (Ishizaki et al., 2015) by the LR reaction (Gateway cloning technology; Invitrogen). To construct a gene for MpphotC328A/C628A, the C628A mutation was introduced into the pENTR‐gMpPHOTC328A plasmid by DpnI‐mediated site‐directed mutagenesis, as previously described (Fujii et al., 2017). The resulting pENTR‐gMpPHOTC328A/C628A plasmid was mixed with the binary vector pMpGWB307 (Ishizaki et al., 2015), and the LR reaction (Gateway cloning technology; Invitrogen) was performed to produce pMpGWB307‐gMpPHOTC328A/C628A. The pENTR‐gMpPHOTC328A and pENTR‐gMpPHOTC628A plasmids (Fujii et al., 2017) were mixed with the binary vector pMpGWB307 (Ishizaki et al., 2015) for the LR reaction (Gateway cloning technology; Invitrogen) to produce pMpGWB307‐gMpPHOTC328A and pMpGWB307‐gMpPHOTC628A, respectively. To construct a gene for kinase‐deficient (Kinase‐Dead) Mpphot, the D922N mutation was introduced into the pDONR207‐MpPHOT plasmid by DpnI‐mediated site‐directed mutagenesis with the following primers: 5′‐GTGCAGCTCACTAATTTCGACCTTTCCTTC‐3′ and 5′‐GAAGGAAAGGTCGAAATTAGTGAGCTGCAC‐3′. The resulting pDONR207‐MpPHOTD922N plasmid was mixed with the binary vector pMpGWB306 (Ishizaki et al., 2015), and the LR reaction (Gateway cloning technology; Invitrogen) was performed. MpPHOT‐Dendra2 was designed as a Gateway entry clone by us and synthesized by Fasmac Co Ltd. The resulting plasmid (pUCFa vector backbone: Fasmac Co Ltd) was mixed with the binary vector pMpGWB302 (Ishizaki et al., 2015), and the LR reaction (Gateway cloning technology; Invitrogen) was performed. The sequence of MpPHOT‐Dendra2 in the pUCFa vector is shown in Figure S7.” The authors apologize for the error from their published article.
  1 in total

1.  Relationship between relocation of phototropin to the chloroplast periphery and the initiation of chloroplast movement in Marchantia polymorpha.

Authors:  Momoko Sakata; Shun Kimura; Yuta Fujii; Takamasa Sakai; Yutaka Kodama
Journal:  Plant Direct       Date:  2019-08-27
  1 in total

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