Subtyping of orosomucoid 1 (ORM1) by isoelectric focusing in agarose and polyacrylamide gels.

Genetic polymorphism of orosomucoid (ORM) has been demonstrated in several populations and comprises two structural gene loci, ORM1 and ORM2. In Caucasians three common ORM1 alleles have been shown, while the ORM2 locus is almost monomorphic. ORM1 phenotyping by isoelectric focusing in agarose or polyacrylamide gel combined with either print immunofixation or enzyme-linked immunoblotting is described, and population and family data from Denmark and Southern Germany are given. It is proposed to use a different alpha-numerical nomenclature for the phenotypes of the ORM1 and ORM2 systems.