M Nita1, J Pliszczyński1, C Kowalewski2, K Woźniak2, A Eljaszewicz3, M Moniuszko4, A Kamiński5, D Śladowski5, Z Zimek6, S Majewski2, M Kosieradzki1, P Fiedor7. 1. Department of General and Transplantation Surgery, Medical University of Warsaw, Warsaw, Poland. 2. Department of Dermatology, Medical University of Warsaw, Warsaw, Poland. 3. Department of Regenerative Medicine and Immune Regulation, Medical University of Białystok, Białystok, Poland. 4. Department of Regenerative Medicine and Immune Regulation, Medical University of Białystok, Białystok, Poland; Department of Allergology and Internal Medicine, Medical University of Białystok, Białystok, Poland. 5. Department of Transplantology and Central Tissue Bank, Medical University of Warsaw, Warsaw, Poland. 6. Institute of Nuclear Chemistry and Technology, Warsaw, Poland. 7. Department of General and Transplantation Surgery, Medical University of Warsaw, Warsaw, Poland. Electronic address: piotrfiedor@wp.pl.
Abstract
BACKGROUND: Nonhealing wounds can be a major clinical problem. Impaired wound healing is often related to massive tissue injury, concomitant wound healing deficiencies (chronic wounds), burn injury, or congenital conditions. We propose a novel biological dressing as an alternative surgical approach. The dressing is a form of an allogenic human skin graft equivalent with further use of allogeneic stem cells classified as an advanced therapy medicinal product. This new allogenic acellular human skin graft has been specifically developed to address the clinical indications for dressing wound lesions and promoting tissue repair in specific rare genetic diseases. METHODS: This case report illustrates the use of an acellular human skin allograft seeded with multipotent stem cells in the treatment of tissue injuries (burns), congenital conditions, and chronic wounds. Donor-tissue processing yields an acellular dermal matrix with integral collagen bundling and organization, as well as an intact basement membrane complex. RESULTS: Preclinical observations show prolonged viability of acellular human skin grafts with multipotent stem cells. This was confirmed with histological and electron-microscopic evaluation of biopsies, which demonstrated host-cell infiltration and neovascularization of the biological dressing. Moreover, the dressings were characterized by low immunogenicity, as confirmed by histology exam and T-cell proliferation assays in vitro. CONCLUSION: Our data confirmed the safety and efficacy of the evaluated acellular human skin grafts, which may be used in patients with rare diseases, such as epidermolysis bullosa, burn injuries, and chronic wounds.
BACKGROUND: Nonhealing wounds can be a major clinical problem. Impaired wound healing is often related to massive tissue injury, concomitant wound healing deficiencies (chronic wounds), burn injury, or congenital conditions. We propose a novel biological dressing as an alternative surgical approach. The dressing is a form of an allogenic human skin graft equivalent with further use of allogeneic stem cells classified as an advanced therapy medicinal product. This new allogenic acellular human skin graft has been specifically developed to address the clinical indications for dressing wound lesions and promoting tissue repair in specific rare genetic diseases. METHODS: This case report illustrates the use of an acellular human skin allograft seeded with multipotent stem cells in the treatment of tissue injuries (burns), congenital conditions, and chronic wounds. Donor-tissue processing yields an acellular dermal matrix with integral collagen bundling and organization, as well as an intact basement membrane complex. RESULTS: Preclinical observations show prolonged viability of acellular human skin grafts with multipotent stem cells. This was confirmed with histological and electron-microscopic evaluation of biopsies, which demonstrated host-cell infiltration and neovascularization of the biological dressing. Moreover, the dressings were characterized by low immunogenicity, as confirmed by histology exam and T-cell proliferation assays in vitro. CONCLUSION: Our data confirmed the safety and efficacy of the evaluated acellular human skin grafts, which may be used in patients with rare diseases, such as epidermolysis bullosa, burn injuries, and chronic wounds.
Authors: Jurandir Tomaz de Miranda; Fabiana de Andrade Bringel; Ana Paula Pereira Velosa; Verônica Protocevich; Sandra de Morais Fernezlian; Pedro Leme Silva; Vera Luiza Capelozzi; Monica Beatriz Mathor; Walcy Rosolia Teodoro Journal: PLoS One Date: 2022-01-27 Impact factor: 3.240
Authors: Jordan Holl; Cezary Kowalewski; Zbigniew Zimek; Piotr Fiedor; Artur Kaminski; Tomasz Oldak; Marcin Moniuszko; Andrzej Eljaszewicz Journal: Cells Date: 2021-03-15 Impact factor: 6.600