| Literature DB >> 32339858 |
Qixin Chen1, Xintian Shao2, Mingang Hao3, Hongbao Fang3, Ruilin Guan4, Zhiqi Tian3, Miaoling Li5, Chenran Wang3, Liangnian Ji4, Hui Chao6, Jun-Lin Guan7, Jiajie Diao8.
Abstract
Super-resolution optical microscopy has extended the spatial resolution of cell biology from the cellular level to the nanoscale, enabling the observation of the interactive behavior of single mitochondria and lysosomes. Quantitative parametrization of interactions between mitochondria and lysosomes under super-resolution optical microscopy, however, is currently unavailable, which has severely limited our understanding of the molecular machinery underlying mitochondrial functionality. Here, we introduce an M-value to quantitatively investigate mitochondria and lysosome contact (MLC) and mitophagy under structured illumination microscopy. We found that the M-value for an MLC is typically less than 0.4, whereas in mitophagy it ranges from 0.5 to 1.0. This system permits further investigation of the detailed molecular mechanism governing the interactive behavior of mitochondria and lysosomes.Entities:
Keywords: Lysosome; Membrane fusion; Mitochondria; Mitophagy; Super-resolution imaging
Mesh：
Year: 2020 PMID： 32339858 PMCID： PMC7236803 DOI： 10.1016/j.biomaterials.2020.120059
Source DB: PubMed Journal: Biomaterials ISSN： 0142-9612 Impact factor: 12.479