Literature DB >> 323365

Use of antibody-coated red cells for the sensitive detection of antigen and in rosette tests for cells bearing surface immunoglobulins.

N R Ling, S Bishop.   

Abstract

Conditions for an improved chromic chloride method for the attachment of antibody to red cells are described. The method, which is applicable to whole Ig fractions as well as affinity-purified antibodies, is reproducible and has a sensitivity for detection of antigen in the nanogram range. The coated cells may be used in a rosette assay for the detection of cell surface-bound antigens.

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Year:  1977        PMID： 323365     DOI： 10.1016/0022-1759(77)90065-5

Source DB:  PubMed          Journal:  J Immunol Methods        ISSN： 0022-1759            Impact factor:   2.303


  68 in total

1.  Immunogenic and antigenic epitopes of immunoglobulins. XIV. Antigenic variants of IgG4 proteins revealed with monoclonal antibodies.

Authors:  M R Walker; P Bird; D O Ulaeto; F Vartdal; D M Goodall; R Jefferis
Journal:  Immunology       Date:  1986-01       Impact factor: 7.397

2.  Immunogenic and antigenic epitopes of immunoglobulins. XVIII. Subpopulations of human lambda chains defined with a panel of monoclonal antibodies.

Authors:  M R Walker; A Solomon; N R Ling; B Brown; J A Lowe; D Hardie; R Jefferis
Journal:  Immunology       Date:  1986-11       Impact factor: 7.397

3.  Immunogenicity and antigenicity of immunoglobulins. XII. Intact light chain and heavy chain isotype-restricted Vk-associated epitopes.

Authors:  M Walker; D Hardie; J Lowe; N R Ling; G De Lange; R Jefferis
Journal:  Immunology       Date:  1985-06       Impact factor: 7.397

4.  Coexpression of Fc gamma receptor IIIA and interleukin-2 receptor beta chain by a subset of human CD3+/CD8+/CD11b+ lymphocytes.

Authors:  S Zupo; L Azzoni; R Massara; A D'Amato; B Perussia; M Ferrarini
Journal:  J Clin Immunol       Date:  1993-05       Impact factor: 8.317

5.  Immune function in systemic lupus erythematosus. Impairment of in vitro T-cell proliferation and in vivo antibody response to exogenous antigen.

Authors:  A B Gottlieb; R G Lahita; N Chiorazzi; H G Kunkel
Journal:  J Clin Invest       Date:  1979-05       Impact factor: 14.808

6.  Characterization of the receptor for IgM present on human B lymphocytes.

Authors:  G F Burns; J C Cawley; C R Barker
Journal:  Immunology       Date:  1979-03       Impact factor: 7.397

7.  The relationship of HNK-1 (Leu 7) and VEP13 antigens on human cells mediating natural killing.

Authors:  H Rumpold; D Kraft; G Gastl; C Huber
Journal:  Clin Exp Immunol       Date:  1984-09       Impact factor: 4.330

8.  Preparation of a monoclonal antibody to rat liver glucose-6-phosphate dehydrogenase and the study of its immunoreactivity with native and inactivated enzyme.

Authors:  M L Dao; B C Johnson; P E Hartman
Journal:  Proc Natl Acad Sci U S A       Date:  1982-05       Impact factor: 11.205

9.  Cellular origins of serum complement receptor type 2 in normal individuals and in hypogammaglobulinaemia.

Authors:  N Ling; T Hansel; P Richardson; B Brown
Journal:  Clin Exp Immunol       Date:  1991-04       Impact factor: 4.330

10.  Seronegative rheumatoid arthritis, rheumatoid factor cross reactive idiotype expression, and hidden rheumatoid factors.

Authors:  V R Bonagura; J F Wedgwood; N Agostino; L Hatam; L Mendez; I Jaffe; B Pernis
Journal:  Ann Rheum Dis       Date:  1989-06       Impact factor: 19.103
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