Abdullah Munir Roslan1, Afiqah Mustafa Kamil2, Carumathy Chandran2, Adelene Ai-Lian Song3,4, Khatijah Yusoff2,5, Raha Abdul Rahim1,5,6. 1. Department of Cell and Molecular Biology, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, 43400, Serdang, Selangor, Malaysia. 2. Department of Microbiology, Faculty of Biotechnology & Biomolecular Sciences, Universiti Putra Malaysia, 43400, Serdang, Selangor, Malaysia. 3. Department of Microbiology, Faculty of Biotechnology & Biomolecular Sciences, Universiti Putra Malaysia, 43400, Serdang, Selangor, Malaysia. adelene@upm.edu.my. 4. Institute of Bioscience, Universiti Putra Malaysia, 43400, Serdang, Selangor, Malaysia. adelene@upm.edu.my. 5. Institute of Bioscience, Universiti Putra Malaysia, 43400, Serdang, Selangor, Malaysia. 6. Chancellory, Universiti Teknikal Malaysia Melaka, Hang Tuah Jaya, Durian Tunggal, 76100, Melaka, Malaysia.
Abstract
OBJECTIVE: The effect of two signal peptides, namely Usp45 and Spk1 on the secretion of xylanase in Lactococcus lactis was analysed. RESULTS: Xylanase was successfully expressed in Lactococcus lactis. Recombinant xylanase fused to either signal peptide Usp45 or Spk1 showed halo zone on Remazol Brilliant Blue-Xylan plates. This indicated that the xylanase was successfully secreted from the cell. The culture supernatants of strains secreting the xylanase with help of the Spk1 and Usp45 signal peptides contained 49.7 U/ml and 34.4 U/ml of xylanase activity, respectively. CONCLUSION: Although Usp45 is the most commonly used signal peptide when secreting heterologous proteins in Lactococcus lactis, this study shows that Spk1 isolated from Pediococcus pentosaceus was superior to Usp45 in regard to xylanase protein secretion.
OBJECTIVE: The effect of two signal peptides, namely Usp45 and Spk1 on the secretion of xylanase in Lactococcus lactis was analysed. RESULTS: Xylanase was successfully expressed in Lactococcus lactis. Recombinant xylanase fused to either signal peptide Usp45 or Spk1 showed halo zone on Remazol Brilliant Blue-Xylan plates. This indicated that the xylanase was successfully secreted from the cell. The culture supernatants of strains secreting the xylanase with help of the Spk1 and Usp45 signal peptides contained 49.7 U/ml and 34.4 U/ml of xylanase activity, respectively. CONCLUSION: Although Usp45 is the most commonly used signal peptide when secreting heterologous proteins in Lactococcus lactis, this study shows that Spk1 isolated from Pediococcus pentosaceus was superior to Usp45 in regard to xylanase protein secretion.
Entities:
Keywords:
Lactococcus lactis; Secretion; Signal peptides; Xylanase
Authors: Nur Aqlili Riana Alias; Adelene Ai-Lian Song; Noorjahan Banu Alitheen; Raha Abdul Rahim; Siti Sarah Othman; Lionel Lian Aun In Journal: Int J Mol Sci Date: 2022-09-02 Impact factor: 6.208