Biochemical characterization and degradation pattern analysis of a novel PL-6 alginate lyase from Streptomyces coelicolor A3(2).

Alginate is the main component of brown algae which contributes to a huge biomass. The alginate oligosaccharides (AOs) have been widely used in food, cosmetic and pharmaceutical industries due to their various physiological activities. In this study, we expressed and characterized a novel PL-6 alginate lyase, named OUC-ScCD6. The results indicated that OUC-ScCD6 showed highest activity at 50 °C and pH 9.0. OUC-ScCD6 prefers to degrade poly M blocks and could digest poly G blocks as well. Endolytic action mode towards polysaccharides contributes to the creation of AOs with the degrees of polymerization 2-6. Degradation towards saturated oligosaccharides showed that saturated trisaccharides (M3 and G3) were minimum identifiable substrates. Furthermore, OUC-ScCD6 shows an even-numbered glycosidic bonds preference from non-reducing end which provided clearer insights into the substrate recognition and action mode of PL-6 family alginate lyases.