K Snehaa1, Taru Singh1, Sajad Ahmad Dar2, Shafiul Haque3, Vishnampettai G Ramachandran1, Rumpa Saha1, Dheeraj Shah4, Shukla Das5. 1. Department of Microbiology, University College of Medical Sciences (University of Delhi), Guru Teg Bahadur Hospital, Delhi, 110095, India. 2. Department of Microbiology, University College of Medical Sciences (University of Delhi), Guru Teg Bahadur Hospital, Delhi, 110095, India; Research and Scientific Studies Unit, College of Nursing & Allied Health Sciences, Jazan University, Jazan, 45142, Saudi Arabia. 3. Research and Scientific Studies Unit, College of Nursing & Allied Health Sciences, Jazan University, Jazan, 45142, Saudi Arabia. 4. Department of Paediatrics, University College of Medical Sciences (University of Delhi), Guru Teg Bahadur Hospital, Delhi, 110095, India. 5. Department of Microbiology, University College of Medical Sciences (University of Delhi), Guru Teg Bahadur Hospital, Delhi, 110095, India. Electronic address: shukladas_123@yahoo.com.
Abstract
BACKGROUND: Worldwide around 2 million deaths occur every year due to diarrhoeal illnesses among children less than 5 years of age. Among diarrhoeagenic Escherichia coli, Enteropathogenic E. coli (EPEC) is highly prevalent in both community and hospital settings and is one of the main causes of persistent diarrhea in children in developing countries. EPEC remains underdiagnosed in India due to lack of conventional tool for identification. MATERIALS AND METHODS: We in this study investigated the prevalence and regional variation of EPEC in paediatric population suffering from diarrhoea in East Delhi, India. Two hundred stool samples were collected from children, aged between 0.5 and 5 years, with acute diarrhoea. E. coli were identified by conventional tests and PCR. RESULTS: We observed 7% atypical EPEC (aEPEC) and 2.5% typical EPEC (tEPEC), with an overall 9.5% EPEC prevalence amongst total samples. E. coli phylogenetic group A was the predominant. The most common age group affected was 6-23 months with common symptoms being vomiting, watery diarrhoea and severe dehydration. High drug resistance pattern was observed in EPEC isolates. CONCLUSION: The study depicts a changing trend of aEPEC over tEPEC in children less than 5 years with diarrhoea, an emerging drug resistant enteropathogen and a public health concern demanding monitoring and surveillance.
BACKGROUND: Worldwide around 2 million deaths occur every year due to diarrhoeal illnesses among children less than 5 years of age. Among diarrhoeagenic Escherichia coli, Enteropathogenic E. coli (EPEC) is highly prevalent in both community and hospital settings and is one of the main causes of persistent diarrhea in children in developing countries. EPEC remains underdiagnosed in India due to lack of conventional tool for identification. MATERIALS AND METHODS: We in this study investigated the prevalence and regional variation of EPEC in paediatric population suffering from diarrhoea in East Delhi, India. Two hundred stool samples were collected from children, aged between 0.5 and 5 years, with acute diarrhoea. E. coli were identified by conventional tests and PCR. RESULTS: We observed 7% atypical EPEC (aEPEC) and 2.5% typical EPEC (tEPEC), with an overall 9.5% EPEC prevalence amongst total samples. E. coli phylogenetic group A was the predominant. The most common age group affected was 6-23 months with common symptoms being vomiting, watery diarrhoea and severe dehydration. High drug resistance pattern was observed in EPEC isolates. CONCLUSION: The study depicts a changing trend of aEPEC over tEPEC in children less than 5 years with diarrhoea, an emerging drug resistant enteropathogen and a public health concern demanding monitoring and surveillance.
Authors: Rasheed Omotayo Adeyemo; Ibukun Michael Famuyide; Jean Paul Dzoyem; McGaw Lyndy Joy Journal: Evid Based Complement Alternat Med Date: 2022-09-28 Impact factor: 2.650