Dan Xing1,2, Jun Wu3,4,5, Bin Wang6, Wei Liu7,8, Wenjing Liu3,4, Yu Zhao1,2, Liu Wang3,4,5,9, Jiao Jiao Li10, Aifeng Liu11, Qi Zhou3,4,5,9, Jie Hao3,4,5, Jianhao Lin1,2. 1. Arthritis Clinic & Research Center, Peking University People's Hospital, Peking University, Beijing, China. 2. Arthritis Institute, Peking University, Beijing, China. 3. State Key Laboratory of Stem Cell and Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing, China. 4. Beijing Stem Cell Bank, Chinese Academy of Sciences, Beijing, China. 5. University of Chinese Academy of Sciences, Beijing, China. 6. Department of Sports Medicine and Adult Reconstruction Surgery, Nanjing Drum Tower Hospital, The Affiliated Hospital of Nanjing University Medical School, Nanjing, China. 7. Department of Biomedical Engineering, School of Medicine, Tsinghua University, Beijing, China. 8. Beijing CytoNiche Biotechnology Co. Ltd., Beijing, China. 9. Institute for Stem Cell and Regeneration, Chinese Academy of Sciences, Beijing, China. 10. Kolling Institute, University of Sydney, Sydney, NSW, Australia. 11. Department of Orthopedics, First Teaching Hospital of Tianjin University of Traditional Chinese Medicine, Tianjin, China.
Abstract
AIM: Mesenchymal stem cell (MSC)-based therapy is being explored in treating osteoarthritis (OA). Human umbilical cord-derived mesenchymal stem cells (hUC-MSCs) are least reported. In this study, we investigated the effects of single intra-articular injections of hUC-MSCs on a rat OA model. METHOD: hUC-MSCs were isolated from the Wharton's jelly of the human umbilical cord and identified. Eighteen Sprague-Dawley rats were used for the OA model. All rats were divided into 3 groups: hyaluronic acid (HA)+MSCs (n = 6), HA (n = 6), and control group (n = 6). One by 106 hUC-MSCs in 100 μL HA, 100 μL HA or 100 μL saline were injected into the knee joint 4 weeks post-surgery as a single dose. Cartilage degeneration was evaluated at 6 and 12 weeks after treatment with macroscopic examination, micro-computed tomography analysis, behavioral analysis, and histology. RESULTS: At 6 weeks, the HA + MSCs group had a significantly better International Cartilage Repair Society score in the femoral condyle compared to the HA and control groups. Histological analysis also showed more proteoglycan and less cartilage loss, with lower modified Mankin score in the HA + MSCs group. However, at 12 weeks there were no significant differences between groups from macroscopic examination and histological analysis. Subchondral bone sclerosis of the medial femoral condyle and behavioral tests showed no significant differences between groups at 6 and 12 weeks. CONCLUSION: These findings indicate that single injection of hUC-MSCs can have temporary effects on decelerating the progression of cartilage degeneration in OA rats, but may not inhibit OA progression in the long-term.
AIM: Mesenchymal stem cell (MSC)-based therapy is being explored in treating osteoarthritis (OA). Human umbilical cord-derived mesenchymal stem cells (hUC-MSCs) are least reported. In this study, we investigated the effects of single intra-articular injections of hUC-MSCs on a rat OA model. METHOD: hUC-MSCs were isolated from the Wharton's jelly of the human umbilical cord and identified. Eighteen Sprague-Dawley rats were used for the OA model. All rats were divided into 3 groups: hyaluronic acid (HA)+MSCs (n = 6), HA (n = 6), and control group (n = 6). One by 106 hUC-MSCs in 100 μL HA, 100 μL HA or 100 μL saline were injected into the knee joint 4 weeks post-surgery as a single dose. Cartilage degeneration was evaluated at 6 and 12 weeks after treatment with macroscopic examination, micro-computed tomography analysis, behavioral analysis, and histology. RESULTS: At 6 weeks, the HA + MSCs group had a significantly better International Cartilage Repair Society score in the femoral condyle compared to the HA and control groups. Histological analysis also showed more proteoglycan and less cartilage loss, with lower modified Mankin score in the HA + MSCs group. However, at 12 weeks there were no significant differences between groups from macroscopic examination and histological analysis. Subchondral bone sclerosis of the medial femoral condyle and behavioral tests showed no significant differences between groups at 6 and 12 weeks. CONCLUSION: These findings indicate that single injection of hUC-MSCs can have temporary effects on decelerating the progression of cartilage degeneration in OA rats, but may not inhibit OA progression in the long-term.
Authors: Robert Köhnke; Marcus Oliver Ahlers; Moritz Alexander Birkelbach; Florian Ewald; Michael Krueger; Imke Fiedler; Björn Busse; Max Heiland; Tobias Vollkommer; Martin Gosau; Ralf Smeets; Rico Rutkowski Journal: Int J Mol Sci Date: 2021-01-05 Impact factor: 5.923
Authors: Dan Xing; Kai Wang; Jun Wu; Yu Zhao; Wei Liu; Jiao Jiao Li; Tingting Gao; Deng Yan; Liu Wang; Jie Hao; Jianhao Lin Journal: Molecules Date: 2021-01-24 Impact factor: 4.411