Yun Wang1, Xu-Dong Xie2, Chun-Mei Xu2, Jun Wang2. 1. State Key Laboratory of Oral Diseases & National Clinical Research Center for Oral Diseases & West China Hospital of Stomatology, Sichuan University, Chengdu 610041, China. 2. State Key Laboratory of Oral Diseases & National Clinical Research Center for Oral Diseases & Dept. of Periodontics, West China Hospital of Stomatology, Sichuan University, Chengdu 610041, China.
Abstract
OBJECTIVE: This study aimed to investigate the distribution of Gli1+ cells in the periodontal ligament (PDL) and to evaluate their contribution in the development of periodontal tissue by using transgenic mouse lines. METHODS: Gli1lacZ/+ mice were harvested at different ages (3, 6, and 8 weeks), and the temporal and spatial distribution patterns of Gli1+ PDL cells were revealed by X-gal staining. Afterward, 3-week-old Gli1-CreERT2/+;R26RtdTomato/+ mice were administered with tamoxifen, and the fates of Gli1+ cells and their descendants were traced during periodontal development. RESULTS: A large number of Gli1+ cells were detected in the PDL of the 3-week-old mice; however, their number significantly decreased from 3 weeks to 8 weeks (P<0.05). Cell lineage tracing data showed that the descendants of Gli1+ cells dramatically increased from 3 weeks to 8 weeks (P<0.05) and gradually differentiated into fibroblasts, cementocytes, and osteocytes. CONCLUSIONS: The multi-differentiation potential of Gli1+ PDL cells was revealed, indicating that Gli1+ cells are an important cell source for periodontal development.
OBJECTIVE: This study aimed to investigate the distribution of Gli1+ cells in the periodontal ligament (PDL) and to evaluate their contribution in the development of periodontal tissue by using transgenic mouse lines. METHODS: Gli1lacZ/+ mice were harvested at different ages (3, 6, and 8 weeks), and the temporal and spatial distribution patterns of Gli1+ PDL cells were revealed by X-gal staining. Afterward, 3-week-old Gli1-CreERT2/+;R26RtdTomato/+ mice were administered with tamoxifen, and the fates of Gli1+ cells and their descendants were traced during periodontal development. RESULTS: A large number of Gli1+ cells were detected in the PDL of the 3-week-old mice; however, their number significantly decreased from 3 weeks to 8 weeks (P<0.05). Cell lineage tracing data showed that the descendants of Gli1+ cells dramatically increased from 3 weeks to 8 weeks (P<0.05) and gradually differentiated into fibroblasts, cementocytes, and osteocytes. CONCLUSIONS: The multi-differentiation potential of Gli1+ PDL cells was revealed, indicating that Gli1+ cells are an important cell source for periodontal development.
Authors: Masako Miura; Stan Gronthos; Mingrui Zhao; Bai Lu; Larry W Fisher; Pamela Gehron Robey; Songtao Shi Journal: Proc Natl Acad Sci U S A Date: 2003-04-25 Impact factor: 11.205
Authors: Ubele van der Velden; Amaliya Amaliya; Bruno G Loos; Mark F Timmerman; Fridus A van der Weijden; Edwin G Winkel; Frank Abbas Journal: J Clin Periodontol Date: 2015-09-16 Impact factor: 8.728
Authors: Faiyaz Notta; Sergei Doulatov; Elisa Laurenti; Armando Poeppl; Igor Jurisica; John E Dick Journal: Science Date: 2011-07-08 Impact factor: 47.728
Authors: C Morsczeck; W Götz; J Schierholz; F Zeilhofer; U Kühn; C Möhl; C Sippel; K H Hoffmann Journal: Matrix Biol Date: 2005-02-12 Impact factor: 11.583
Authors: Sunil S Karhadkar; G Steven Bova; Nadia Abdallah; Surajit Dhara; Dale Gardner; Anirban Maitra; John T Isaacs; David M Berman; Philip A Beachy Journal: Nature Date: 2004-09-12 Impact factor: 49.962