[Chromatographic separation of mono- and oligomeric carbohydrates from biological materials with the use of HPLC glass columns].

By means of HPLC mono- and oligomeric carbohydrates are separated on silica, modified chemically with aminopropyl groups or impregnated in situ with an amine modifier (piperazine). Fundamentals of this sugar analysis are self-packed glass columns. The advantages of these columns are high efficiencies and handy column plugs. They are chemically inert and can be packed in different length with different HPLC packings. Applications of HPLC technique using glass columns are demonstrated by way of qualitative and quantitative determinations of carbohydrates in different food-stuffs and in polysaccharides (e.g. starch, regenerated fibre), hydrolyzed under acid and enzymatic conditions.