Electrode designed with a nanocomposite film of CuO Honeycombs/Ag nanoparticles electrogenerated on a magnetic platform as an amperometric glucose sensor.

Here, an ultrasensitive non-enzymatic glucose sensor was fabricated using a facile and low price electro-deposition method. At first, thiol-functionalized magnetic nanoparticles cast onto the glassy carbon electrode (GCE) surface to provide a stable substrate with the high surface area. Then, CuO nanoparticles and Ag nanoparticles electrodeposit on the surface of Fe3O4-SH/GCE to obtain the final modified GCE. The characterization of electro-synthesized nanoparticles and the modified GCE was done by different related techniques such as field emission scanning electronic microscopy (FESEM), energy-dispersive X-ray spectroscopy (EDX), cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS). The proposed electrode was applied to the electrochemical sensing of glucose. By employing the optimum conditions on the preparation of modified electrode such as time and potential for electrosynthesis of two different nanoparticles, high reproducibility of measurement and sensor preparation were achieved. The thus optimized simple glucose sensor could be provided stable responses in a wide linear range from 0.06 to 1000 μM with detection limit 15 nM, indicating its potential application for real biological samples.