Mirko Lischer1,2,3, Pietro G di Summa4, Carlo M Oranges1, Dirk J Schaefer1,5, Daniel F Kalbermatten1,2,3, Raphael Guzman5,6, Srinivas Madduri1,2,3,5. 1. Department of Plastic, Reconstructive, Aesthetic & Hand Surgery, University Hospital Basel, Spitalstrasse 21, 4031, Basel, Switzerland. 2. Department of Pathology, University Hospital Basel, Hebelstrasse 20, 4021, Basel, Switzerland. 3. Department of Biomedical Engineering, University of Basel, 4123, Allschwil, Basel, Switzerland. 4. Department of Plastic & Hand Surgery, University Hospital Lausanne (CHUV), 1005, Lausanne, Switzerland. 5. Department of Biomedicine, University of Basel, Hebelstrasse 20, 4021, Basel, Switzerland. 6. Department of Neurosurgery, University Hospital Basel, Spitalstrasse 21, 4031, Basel, Switzerland.
Abstract
Aim: We investigated a potential strategy involving human platelet lysate (HPL) as a media additive for enhancing the neurotrophic potency of human adipose stem cells (ASC). Materials & methods: Dorsal root ganglion explants, ASC and Schwann cells were used for in vitro axonal outgrowth experiments. Results: Remarkably, HPL-supplemented ASC promoted robust axonal outgrowth, in other words, four-times higher than fetal bovine serum-supplemented ASC and even matched to the level of Schwann cells. Further, analysis of regime of growth medium additive supplementation revealed the critical play of HPL in dorsal root ganglion and stem cells co-culture system for mounting effective axonal growth response. Conclusion: HPL supplementation significantly improved the neurotrophic potency of ASC as evidenced by the robust axonal outgrowth; these findings hold significance for nerve tissue engineering applications.
Aim: We investigated a potential strategy involving human platelet lysate (HPL) as a media additive for enhancing the neurotrophic potency of human adipose stem cells (ASC). Materials & methods: Dorsal root ganglion explants, ASC and Schwann cells were used for in vitro axonal outgrowth experiments. Results: Remarkably, HPL-supplemented ASC promoted robust axonal outgrowth, in other words, four-times higher than fetal bovine serum-supplemented ASC and even matched to the level of Schwann cells. Further, analysis of regime of growth medium additive supplementation revealed the critical play of HPL in dorsal root ganglion and stem cells co-culture system for mounting effective axonal growth response. Conclusion:HPL supplementation significantly improved the neurotrophic potency of ASC as evidenced by the robust axonal outgrowth; these findings hold significance for nerve tissue engineering applications.
Authors: Silvia Palombella; Martino Guiotto; Gillian C Higgins; Laurent L Applegate; Wassim Raffoul; Mario Cherubino; Andrew Hart; Mathis O Riehle; Pietro G di Summa Journal: Stem Cell Res Ther Date: 2020-10-06 Impact factor: 6.832