| Literature DB >> 32306334 |
Abstract
Cation-dependent mannose 6-phosphate receptor (CD-MPR) and cation-independent MPR (CI-MPR) belong to the P-type lectin family. Both intracellular and cell surface MPRs can recognize and bind with the terminal mannose 6-phospahte (M6P) residues of N-glycans attached to the mammalian lysosomal enzymes and the related co-factors. Domain9 (Dom9), which is one of the extracytoplasmic region of the CI-MPR, has relatively higher affinity for M6P residues. Here we describe the production of recombinant Dom9-His protein by Pichia pastris, purification, and application as a probe for lectin blotting.Entities:
Keywords: CI-MPR; Dom9; His-tag; Lectin blotting; M6P residue; Yeast Pichia pastris
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Year: 2020 PMID： 32306334 DOI： 10.1007/978-1-0716-0430-4_26
Source DB: PubMed Journal: Methods Mol Biol ISSN： 1064-3745