Currently, little is known about the molecular interactions between SARS-CoV-2 and its host. In this preprint, Gordon et al. cloned, tagged and expressed 26 of 29 SARS-CoV-2 proteins individually in HEK293T cells and used mass spectrometry to measure protein–protein interactions. They identified 332 interactions between viral and host proteins, and several viral proteins (including N, nsp9, nsp13, nsp15, ORF3a and ORF6) were found to directly target cellular proteins involved in innate immune signalling. Furthermore, they noted 69 existing drugs known to target host proteins or associated pathways that interact with SARS-CoV-2. Overall, this ‘SARS-CoV-2 interaction map’ paves the way to better understand how the virus hijacks its host during infection and provides promising therapeutic candidates.
Authors: David E Gordon; Gwendolyn M Jang; Mehdi Bouhaddou; Jiewei Xu; Kirsten Obernier; Matthew J O'Meara; Jeffrey Z Guo; Danielle L Swaney; Tia A Tummino; Ruth Hüttenhain; Robyn M Kaake; Alicia L Richards; Beril Tutuncuoglu; Helene Foussard; Jyoti Batra; Kelsey Haas; Maya Modak; Minkyu Kim; Paige Haas; Benjamin J Polacco; Hannes Braberg; Jacqueline M Fabius; Manon Eckhardt; Margaret Soucheray; Melanie J Bennett; Merve Cakir; Michael J McGregor; Qiongyu Li; Zun Zar Chi Naing; Yuan Zhou; Shiming Peng; Ilsa T Kirby; James E Melnyk; John S Chorba; Kevin Lou; Shizhong A Dai; Wenqi Shen; Ying Shi; Ziyang Zhang; Inigo Barrio-Hernandez; Danish Memon; Claudia Hernandez-Armenta; Christopher J P Mathy; Tina Perica; Kala B Pilla; Sai J Ganesan; Daniel J Saltzberg; Rakesh Ramachandran; Xi Liu; Sara B Rosenthal; Lorenzo Calviello; Srivats Venkataramanan; Yizhu Lin; Stephanie A Wankowicz; Markus Bohn; Raphael Trenker; Janet M Young; Devin Cavero; Joe Hiatt; Theo Roth; Ujjwal Rathore; Advait Subramanian; Julia Noack; Mathieu Hubert; Ferdinand Roesch; Thomas Vallet; Björn Meyer; Kris M White; Lisa Miorin; David Agard; Michael Emerman; Davide Ruggero; Adolfo García-Sastre; Natalia Jura; Mark von Zastrow; Jack Taunton; Olivier Schwartz; Marco Vignuzzi; Christophe d'Enfert; Shaeri Mukherjee; Matt Jacobson; Harmit S Malik; Danica G Fujimori; Trey Ideker; Charles S Craik; Stephen Floor; James S Fraser; John Gross; Andrej Sali; Tanja Kortemme; Pedro Beltrao; Kevan Shokat; Brian K Shoichet; Nevan J Krogan Journal: bioRxiv Date: 2020-03-22
Authors: Ruchir Gupta; Jacob Charron; Cynthia L Stenger; Jared Painter; Hunter Steward; Taylor W Cook; William Faber; Austin Frisch; Eric Lind; Jacob Bauss; Xiaopeng Li; Olivia Sirpilla; Xavier Soehnlen; Adam Underwood; David Hinds; Michele Morris; Neil Lamb; Joseph A Carcillo; Caleb Bupp; Bruce D Uhal; Surender Rajasekaran; Jeremy W Prokop Journal: J Biol Chem Date: 2020-06-25 Impact factor: 5.157
Authors: Giorgio Frega; Andrea Palloni; Giuseppe Di Pasquale; Gioconda Saccoccio; Alessandro Rizzo; Elisabetta Poluzzi; Primiano Iannone; Giovanni Brandi Journal: Front Pharmacol Date: 2020-10-28 Impact factor: 5.810