Literature DB >> 323035

A comparative study of the differentiation of dissociated nerve cells under different culture conditions.

K Maderspach, M Sensenbrenner.   

Abstract

In vitro differentiation of chick embryo brain cells was compared under several culture conditions. Morphological observations and acetylcholinesterase histochemical staining revealed that the development was similar in all conditions tested if cells have been derived from 7 days embryos. Considering the cultures from 11 days embryos, the cell dissociation by trypsin and the plastic surface proved to be the most favourable conditions in contrast to mechanical dissection and collagen surface.

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Year:  1977        PMID： 323035     DOI： 10.1007/bf02002826

Source DB:  PubMed          Journal:  Experientia        ISSN： 0014-4754


  17 in total

1.  Dissociated cell cultures from fetal mouse hypothalamus. Patterns of organization and ultrastructural features.

Authors:  P Benda; F De Vitry; R Picart; A Tixier-Vidal
Journal:  Exp Brain Res       Date:  1975-07-11       Impact factor: 1.972

2.  Possible roles for cell-to-substratum adhesion in neuronal morphogenesis.

Authors:  P C Letourneau
Journal:  Dev Biol       Date:  1975-05       Impact factor: 3.582

3.  A "DIRECT-COLORING" THIOCHOLINE METHOD FOR CHOLINESTERASES.

Authors:  M J KARNOVSKY; L ROOTS
Journal:  J Histochem Cytochem       Date:  1964-03       Impact factor: 2.479

4.  High cyclic AMP levels in young chick embryonic hearts.

Authors:  M J McLean; R A Lapsley; K Shigenobu; F Murad; N Sperelakis
Journal:  Dev Biol       Date:  1975-01       Impact factor: 3.582

5.  Histochemical studies during the differentiation of dissociated nerve cells cultivated in the presence of brain extracts.

Authors:  M Sensenbrenner; N Springer; J Booher; P Mandel
Journal:  Neurobiology       Date:  1972

6.  Growth and cultivation of dissociated neurons and glial cells from embryonic chick, rat and human brain in flask cultures.

Authors:  J Booher; M Sensenbrenner
Journal:  Neurobiology       Date:  1972

7.  Biochemical and cytochemical studies of the development of choline acetyltransferase and acetylcholinesterase in organotypic cultures of chick neural tube.

Authors:  S U Kim; T H O; E L Wenger
Journal:  J Neurobiol       Date:  1974

8.  Energy metabolism of nerve cells during differentiation. O2 uptake, lactate production and ATP content of chick embryo brain cells before and after cultivation in the Rose chamber.

Authors:  L Dittmann; L Hertz; A Schousboe; H Fosmark; M Sensenbrenner; P Mandel
Journal:  Exp Cell Res       Date:  1973-08       Impact factor: 3.905

9.  Choline acetyltransferase and acetylcholinesterase activities in chicken brain hemispheres in vivo and in cell culture.

Authors:  A Ebel; R Massarelli; M Sensenbrenner; P Mandel
Journal:  Brain Res       Date:  1974-08-23       Impact factor: 3.252

10.  Cultivation and growth of dissociated neurons from chick embryo cerebral cortex in the presence of different substrates.

Authors:  M Sensenbrenner; J Booher; P Mandel
Journal:  Z Zellforsch Mikrosk Anat       Date:  1971
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