Preparation and evaluation of curcumin grafted hyaluronic acid modified pullulan polymers as a functional wound dressing material.

Curcumin grafted hyaluronic acid modified pullulan polymers (Cur-HA-SPu) by chemical conjugation was designed and prepared, and its film may be used to accelerate wound healing and help to fight infection. The synthesis of Cur-HA-SPu polymer was characterized by FT-IR, 1H NMR and DSC. Cur-HA-SPu film has a higher swelling ratio than that of HA-SPu film. Moreover, the good biocompatibility of Cur-HA-SPu polymer was confirmed by skin irritation, cytotoxicity and hemolysis tests. Compared to Cur, the MTT and proliferation test carried out in L929 cells revealed that Cur-HA-SPu polymer showed no cytotoxicity and enhanced cell proliferation. Cur-HA-SPu polymer exhibited a certain bactericidal activity against E. coli and S. aureus. Furthermore, the materials showed antioxidant activity when DPPH method determined. Wound healing study using wistar rat model demonstrated that Cur-HA-SPu film obtained better wound healing result than that of HA-SPu film or natural healing. The above results suggest that Cur-HA-SPu film is a promising and safety formulation for accelerating skin wound healing.