Systematic approach of chromone skeleton for detecting Mg2+, ion: Applications for sustainable cytotoxicity and cell imaging possibilities.

The systematic studies of chromone appended novel chemosensors, favored to Mg2+ ion detection, these were analyzed and characterized by different spectroscopic techniques such as NMR, mass spectroscopy, FTIR and optical techniques. The binding demeanor of the ligands was executed with the library of metal ions and shown the good coordination with Mg2+ ion to the ligand's cavity. Both ligands demonstrated good binding behavior with Mg2+ ion. The ligands represented 1: 1 stoichiometry with Mg2+ ions through Job's plot. The low limit of detection of Mg2+ ion was determined as 2.56 × 10-6 and 1.28 × 10-6 for La and Lb respectively. No interference was occurred in Inference study by foreign metal ions that supported the specific detection of Mg2+ ion among the other metal ions. Further, the cytotoxicity assay test of these chromone appended ligands revealed that both ligands and their respective compound with Mg2+ ion shown negligible toxicity with HeLa cancer cell line. Further, due to the fluorescence properties of the ligands, with or without Mg2+ ion was successfully tested in bioimaging experiment of HeLa cancer cell lines and found that ligands with Mg2+ ions represented good imaging with HeLa cancer cell.