Salum J Lidenge1, Tara Tran2, For Yue Tso2, John R Ngowi3, Danielle M Shea4, Julius Mwaiselage5, Charles Wood6, John T West7. 1. Nebraska Center for Virology, Lincoln, NE, USA; School of Biological Sciences, University of Nebraska-Lincoln, Lincoln, NE, USA; Ocean Road Cancer Institute, Dar es Salaam, Tanzania; Muhimbili University of Health and Allied Sciences, Dar es Salaam, Tanzania. 2. Nebraska Center for Virology, Lincoln, NE, USA; School of Biological Sciences, University of Nebraska-Lincoln, Lincoln, NE, USA. 3. Ocean Road Cancer Institute, Dar es Salaam, Tanzania. 4. Nebraska Center for Virology, Lincoln, NE, USA. 5. Ocean Road Cancer Institute, Dar es Salaam, Tanzania; Muhimbili University of Health and Allied Sciences, Dar es Salaam, Tanzania. 6. Nebraska Center for Virology, Lincoln, NE, USA; School of Biological Sciences, University of Nebraska-Lincoln, Lincoln, NE, USA; Department of Biochemistry, University of Nebraska-Lincoln, Lincoln, NE, USA. Electronic address: jwest2@unl.edu. 7. Nebraska Center for Virology, Lincoln, NE, USA; Department of Biochemistry, University of Nebraska-Lincoln, Lincoln, NE, USA.
Abstract
OBJECTIVE: Kaposi's sarcoma-associated herpesvirus (KSHV) is the causative agent for Kaposi's sarcoma (KS), one of the most common cancers in Tanzania. We have investigated KSHV prevalence and factors associated with KSHV infection in Tanzania. METHODS: This is a cross-sectional study of voluntary blood-donors from Dar es Salaam, Tanzania. Plasma was screened for KSHV, HIV-1, HBV, HCV and Treponema pallidum (syphilis). Associations between KSHV sero-status and risk factors were analyzed. Odds ratios (OR) and 95% confidence intervals (CI) are reported to evaluate risk factors of KSHV infection. All tests were 2-tailed, and P-values <0.05 were considered statistically significant. RESULTS: The overall KSHV seroprevalence was 56.9%. Significantly increased risk of KSHV infection was detected in persons from the Lake and Central Zones (OR=6.4, 95% CI=1.6-25.3, P=0.008 and OR=5.7, 95% CI=1.0-32.5, P=0.048 respectively). A trend toward increased risk of KSHV infection with HIV-1 co-infection was not significant (OR=2.8, 95% CI=1.0-8.0, P=0.06). Seroreactivity to T. pallidum was surprisingly high (14.9%). CONCLUSION: The prevalence of KSHV infection and syphilis was high among Tanzanian blood-donors. The most common transfusion-transmissible infections did not associate with KSHV infection. Regions of focal KSHV infection need further investigation for underappreciated risk factors.
OBJECTIVE:Kaposi's sarcoma-associated herpesvirus (KSHV) is the causative agent for Kaposi's sarcoma (KS), one of the most common cancers in Tanzania. We have investigated KSHV prevalence and factors associated with KSHV infection in Tanzania. METHODS: This is a cross-sectional study of voluntary blood-donors from Dar es Salaam, Tanzania. Plasma was screened for KSHV, HIV-1, HBV, HCV and Treponema pallidum (syphilis). Associations between KSHV sero-status and risk factors were analyzed. Odds ratios (OR) and 95% confidence intervals (CI) are reported to evaluate risk factors of KSHV infection. All tests were 2-tailed, and P-values <0.05 were considered statistically significant. RESULTS: The overall KSHV seroprevalence was 56.9%. Significantly increased risk of KSHV infection was detected in persons from the Lake and Central Zones (OR=6.4, 95% CI=1.6-25.3, P=0.008 and OR=5.7, 95% CI=1.0-32.5, P=0.048 respectively). A trend toward increased risk of KSHV infection with HIV-1 co-infection was not significant (OR=2.8, 95% CI=1.0-8.0, P=0.06). Seroreactivity to T. pallidum was surprisingly high (14.9%). CONCLUSION: The prevalence of KSHV infection and syphilis was high among Tanzanian blood-donors. The most common transfusion-transmissible infections did not associate with KSHV infection. Regions of focal KSHV infection need further investigation for underappreciated risk factors.
Authors: Salum J Lidenge; Andrew V Kossenkov; For Yue Tso; Jayamanna Wickramasinghe; Sara R Privatt; Owen Ngalamika; John R Ngowi; Julius Mwaiselage; Paul M Lieberman; John T West; Charles Wood Journal: PLoS Pathog Date: 2020-07-24 Impact factor: 6.823
Authors: For Yue Tso; Salum J Lidenge; John R Ngowi; Phoebe B Peña; Ashley A Clegg; Owen Ngalamika; Chacha J Mwita; Julius Mwaiselage; Charles Wood Journal: J Clin Med Date: 2022-07-29 Impact factor: 4.964