Hideaki Sumiyoshi1,2, Sachie Nakao1,2, Hitoshi Endo3, Takayo Yanagawa1,2, Yasuhiro Nakano1,2, Yosuke Okamura4, Akira T Kawaguchi2, Yutaka Inagaki1,2,5. 1. Center for Matrix Biology and Medicine, Graduate School of Medicine, Tokai University, Isehara, Japan. 2. Department of Innovative Medical Science, Tokai University School of Medicine, Isehara, Japan. 3. Department of Preventive Medicine, Tokai University School of Medicine, Isehara, Japan. 4. Course of Industrial Chemistry, Graduate School of Engineering, Tokai University, Hiratsuka, Japan. 5. Instutute of Medical Sciences, Tokai University, Isehara, Japan.
Abstract
Background and Objective: Impaired dermal wound healing represents a major medical issue in today's aging populations. Granulation tissue formation in the dermis and reepithelization of the epidermis are both important and necessary for proper wound healing. Although a number of artificial dermal grafts have been used to treat full-thickness dermal loss in humans, they do not induce reepithelization of the wound, requiring subsequent epithelial transplantation. In the present study, we sought a novel biomaterial that accelerates the wound healing process. Approach: We prepared a composite biomaterial made of jellyfish and porcine collagens and developed a hybrid-type dermal graft that composed of the upper layer film and the lower layer sponge made of this composite biomaterial. Its effect on dermal wound healing was examined using a full-thickness excisional wound model. Structural properties of the dermal graft and histological features of the regenerating skin tissue were characterized by electron microscopic observation and immunohistological examination, respectively. Results: The composite biomaterial film stimulated migration of keratinocytes, leading to prompt reepithelization. The regenerating epithelium consisted of two distinct cell populations: keratin 5-positive basal keratinocytes and more differentiated cells expressing tight junction proteins such as claudin-1 and occludin. At the same time, the sponge made of the composite biomaterial possessed a significantly enlarged intrinsic space and enhanced infiltration of inflammatory cells and fibroblasts, accelerating granulation tissue formation. Innovation: This newly developed composite biomaterial may serve as a dermal graft that accelerates wound healing in various pathological conditions. Conclusion: We have developed a novel dermal graft composed of jellyfish and porcine collagens that remarkably accelerates the wound healing process.
Background and Objective: Impaired dermal wound healing represents a major medical issue in today's aging populations. Granulation tissue formation in the dermis and reepithelization of the epidermis are both important and necessary for proper wound healing. Although a number of artificial dermal grafts have been used to treat full-thickness dermal loss in humans, they do not induce reepithelization of the wound, requiring subsequent epithelial transplantation. In the present study, we sought a novel biomaterial that accelerates the wound healing process. Approach: We prepared a composite biomaterial made of jellyfish and porcine collagens and developed a hybrid-type dermal graft that composed of the upper layer film and the lower layer sponge made of this composite biomaterial. Its effect on dermal wound healing was examined using a full-thickness excisional wound model. Structural properties of the dermal graft and histological features of the regenerating skin tissue were characterized by electron microscopic observation and immunohistological examination, respectively. Results: The composite biomaterial film stimulated migration of keratinocytes, leading to prompt reepithelization. The regenerating epithelium consisted of two distinct cell populations: keratin 5-positive basal keratinocytes and more differentiated cells expressing tight junction proteins such as claudin-1 and occludin. At the same time, the sponge made of the composite biomaterial possessed a significantly enlarged intrinsic space and enhanced infiltration of inflammatory cells and fibroblasts, accelerating granulation tissue formation. Innovation: This newly developed composite biomaterial may serve as a dermal graft that accelerates wound healing in various pathological conditions. Conclusion: We have developed a novel dermal graft composed of jellyfish and porcine collagens that remarkably accelerates the wound healing process.
Authors: Anna Sorushanova; Luis M Delgado; Zhuning Wu; Naledi Shologu; Aniket Kshirsagar; Rufus Raghunath; Anne M Mullen; Yves Bayon; Abhay Pandit; Michael Raghunath; Dimitrios I Zeugolis Journal: Adv Mater Date: 2018-08-20 Impact factor: 30.849
Authors: Thomas Volksdorf; Janina Heilmann; Sabine A Eming; Kathrin Schawjinski; Michaela Zorn-Kruppa; Christopher Ueck; Sabine Vidal-Y-Sy; Sabine Windhorst; Manfred Jücker; Ingrid Moll; Johanna M Brandner Journal: Am J Pathol Date: 2017-04-12 Impact factor: 4.307
Authors: Corinna Wicke; Andreas Bachinger; Stephan Coerper; Stefan Beckert; Maria B Witte; Alfred Königsrainer Journal: Wound Repair Regen Date: 2009 Jan-Feb Impact factor: 3.617