PURPOSE: To explore the influence of long non-coding ribonucleic acid (lncRNA) small nucleolar RNA host gene 1 (SNHG1) on the proliferation and apoptosis of gastric cancer cells. METHODS: SNHG1 was knocked down using small-interfering RNAs (siRNAs) in gastric cancer cell line MGC-803 and then the changes in the expression levels of SNHG1 and Notch1 in each group of cells were evaluated via quantitative reverse transcription polymerase chain reaction (qRT-PCR). RESULTS: After intervention of SNHG1 by siRNAs, compared with NC-siRNA group, SNHG1-siRNA group exhibited notably lowered RNA and messenger RNA (mRNA) levels of SNHG1 and Notch1 (p<0.05), a substantially lowered proliferation rate (p<0.05), a remarkably raised apoptosis rate (p<0.05) and markedly decreased protein expression levels of Notch1 and Bax (p<0.05). Additionally, the cells treated with Notch1 inhibitor had the proliferation substantially inhibited (p<0.05), while there was no notable change in the RNA expression of SNHG1 in the cells. CONCLUSIONS: LncRNA SNHG1 can depend on the Notch1 pathway to suppress the proliferation of gastric cancer cells and promote their apoptosis.
PURPOSE: To explore the influence of long non-coding ribonucleic acid (lncRNA) small nucleolar RNA host gene 1 (SNHG1) on the proliferation and apoptosis of gastric cancer cells. METHODS:SNHG1 was knocked down using small-interfering RNAs (siRNAs) in gastric cancer cell line MGC-803 and then the changes in the expression levels of SNHG1 and Notch1 in each group of cells were evaluated via quantitative reverse transcription polymerase chain reaction (qRT-PCR). RESULTS: After intervention of SNHG1 by siRNAs, compared with NC-siRNA group, SNHG1-siRNA group exhibited notably lowered RNA and messenger RNA (mRNA) levels of SNHG1 and Notch1 (p<0.05), a substantially lowered proliferation rate (p<0.05), a remarkably raised apoptosis rate (p<0.05) and markedly decreased protein expression levels of Notch1 and Bax (p<0.05). Additionally, the cells treated with Notch1 inhibitor had the proliferation substantially inhibited (p<0.05), while there was no notable change in the RNA expression of SNHG1 in the cells. CONCLUSIONS: LncRNA SNHG1 can depend on the Notch1 pathway to suppress the proliferation of gastric cancer cells and promote their apoptosis.