Tadanobu Onishi1, Takamasa Shimizu1, Manabu Akahane2, Akinori Okuda1, Tsutomu Kira1, Shohei Omokawa3, Yasuhito Tanaka1. 1. Department of Orthopedic Surgery, Nara Medical University, Kashihara, Nara, 634-8522, Japan. 2. Department of Public Health, Health Management and Policy, Nara Medical University School of Medicine, Kashihara, Nara, 634-8521, Japan. 3. Department of Hand Surgery, Nara Medical University, Kashihara, Nara, 634-8522, Japan.
Abstract
OBJECTIVE: No evidence exists about which biological approach is more reliable for creating non-union model. We investigated how to create a reproducible atrophic non-union model in a rat femur. METHODS: We compared three groups: simple osteotomy (group A), partial periosteum cauterization (group B), and extensive periosteum and bone marrow resection (group C). RESULTS: All samples in group C demonstrated atrophic non-union in radiological, histological, and biomechanical analyses, however half of the samples in group B showed fracture healing at week 16. CONCLUSION: Extensive resection of periosteum and bone marrow is important for a reproducible atrophic non-union model in a rat femur.
OBJECTIVE: No evidence exists about which biological approach is more reliable for creating non-union model. We investigated how to create a reproducible atrophic non-union model in a rat femur. METHODS: We compared three groups: simple osteotomy (group A), partial periosteum cauterization (group B), and extensive periosteum and bone marrow resection (group C). RESULTS: All samples in group C demonstrated atrophic non-union in radiological, histological, and biomechanical analyses, however half of the samples in group B showed fracture healing at week 16. CONCLUSION: Extensive resection of periosteum and bone marrow is important for a reproducible atrophic non-union model in a rat femur.
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