Synthesis, biological activity and molecular modeling study of novel 1,2,4-triazolo[4,3-b][1,2,4,5]tetrazines and 1,2,4-triazolo[4,3-b][1,2,4]triazines.

Different novel 1,2,4-triazolo[4,3-b][1,2,4,5]tetrazines and 1,2,4-triazolo[4,3-b][1,2,4]triazines have been obtained from heterocyclization of 3-substituted-4-amino-5-substituted-amino-1,2,4-triazoles (3a-d) and 3-substituted-4-amino-5-hydrazino-1,2,4-triazoles (9a,b) with (α and β) bifunctional compounds like chloromethyl biphenyl-phosphanoxide, pyruvic acid, phenacyl bromide, diethyl oxalate, triethyl orthoformate, triethyl phosphite, fluorinated benzaldehydes, carbon disulfide and ethyl chloroformate under different experimental settings. Fourier transformer infrared analysis (FTIR), Proton nuclear magnetic resonance (1H NMR) and 13C nuclear magnetic resonance (13C NMR), as well as that of the mass spectral data, were used as the appropriate characterization techniques for the chemical structures of all newly synthesized compounds. The newly prepared compounds were examined as an anti-inflammatory, antibacterial agents (against E. coli (Escherichia coli) and P. aeruginosa (Pseudomonas aeruginosa) as examples for Gram-negative bacteria and S. aureus (Staphylococcus aureus) as examples for Gram-positive bacteria), as well as antifungal (against C. albicans (Candida albicans)) agents. The newly prepared compound showed high antibacterial, antifungal, and anti-inflammatory activities in comparing with the commercial antibiotics Indomethacin, Nalidixic acid, Imipenem, and Nystatin. Docking of the most active compounds was performed depending on the results of antibacterial screening and the anti-inflammatory assay.

Introduction

Studies on heterocyclic compounds containing class="Chemical">bridgehead class="Chemical">pan class="Chemical">nitrogen atom particularly those holding (1,2,4,5)-tetrazine, (1,2,4)-triazole and (1,2,4)-triazine derivatives have received much interest recently as they can be used in a variety of applications, especially in the medicinal field. For example, many of 1,2,4-triazole rings are found into a wide range of pharmaceutical drugs including antimicrobial agents[1,2], antifungal[3,4], antibacterial[5-9], antimycobacterial[10], antiviral[11,12], anticancer[13], antitubercular[14], antimycotic activity[15,16], antimigraini agents, anti-inflammatory and analgesic[17-19], anticonvulsants[20], antinociceptive[21], anti-ureaese[22], antioxidant[23], CNS stimulants, and antidepressant[24] properties.

class="Chemical">1,2,4-triazole rings class="Chemical">possess not only diverse class="Chemical">pharmacological activities but also to have herbicidal, insecticidal, class="Chemical">plant growth regulatory and anticlass="Chemical">pan class="Chemical">fungal activities[25]. Thus, for many years, the biochemistry of these molecules has been investigated[26]. Also, Heterobicyclic nitrogen systems containing 1,2,4-Triazines derivatives and 1,2,4-Triazines themselves have been found to display a diversity of biological applications such as Lamotrigine as anti-epileptic drug[27], Tirapazamine as anti-tumor[28], and fused 1,2,4–triazines as antimicrobial[29,30], anti–viral[31], antimycobacterial[32], anxiolytic[33] and antidepressant[34] activities. They also have shown anti–HIV and anticancer activities[35]. Moreover, derivatives of the tetrazine ring have attracted extensive attention from numerous research groups because of their interesting and diverse biological activities as antitumor and antiviral[36]. In this respect, Abdel-Rahman et al.[37-39] found that 1,2,4-triazines, 1,2,4-triazoles and/or 1,2,4-triazolo-1,2,4,5-tetrazines can be used as a molluscicidal and antimicrobial as well as they have anticancer drugs activity. Due to their novel energetic properties[25,26,40], organic compounds with high-nitrogen content currently attract the attention of many researchers.

In light oclass="Chemical">f these remarks, researchers were class="Chemical">promclass="Chemical">pted to design and synthesize new drugs containing heterocyclic comclass="Chemical">pounds, esclass="Chemical">pecially, those containing class="Chemical">pan class="Chemical">triazoles, triazines and tetrazines rings as a result of the fact that Nitrogen–Nitrogen bond is difficult to be produced in living organisms in addition to its therapeutic activities. Thus, because of these remarks, the target of this work is to synthesize some new 1,2,4-triazole fused with 1,2,4-triazine and/or 1,2,4,5-tetrazine nucleus holding different types of functional groups to enhance their biological activity, in a hope to design a semi-drug. To demonstrate whether COX-II was a potential target for our newly prepared compounds, molecular modeling studies have been conducted on these compounds.

Experimental

General Method for the preparation of novel 1,2,4-triazolo[4,3-b][1,2,4,5]tetrazines and 1,2,4-triazolo[4,3-b][1,2,4]triazines

A mixture oclass="Chemical">f comclass="Chemical">pounds 1a,b, and 2a,b with 1:1 molar class="Chemical">pan class="Species">ratio in 100 mL Ethanol-DMF was refluxed for 5 h, then poured into ice and the formed solids were collected by filtration and crystallized to give compounds 3a to 3d, respectively. To prepare the compounds 5a–d, a mixture of (0.01 mol) of 3a–d and (0.01 mol) of chloromethyl-diphenylphosphanoxide in (100 mL) THF with (0.5 mL) TEA was refluxed for 5 h and cooled. The obtained solids were then were collected by filtration and crystallized to give 5a–d, respectively. When (0.01 mol) of 3a–d were mixed with (0.01 mol) of phenacyl bromide in 50 mL from 5% ethanolic KOH and heated under reflux for 3 h, then poured into ice and acidified with few drops of HCl. The yielded solids were filtered and crystallized to give compounds 6a–d respectively. Compounds 7a–d were prepared by mixing a mixture of (0.01 mol) 3a–d and (0.01 mol) sodium pyruvate, in 10 mL H2O with 50 mL 5% aqueous NaOH. After that, the mixture was refluxed for 3 h, then poured into ice and acidified with few drops of HCl. The products filtered and recrystallized to give 7a–d compounds respectively. Equimolar ratio mixture of both compounds 3a–d and diethyl oxalate in 100 mL THF was refluxed for 5 h and cooled. The obtained products were filtered and crystallized from the proper solvent to give 8a–d respectively. A mixture of each of 1a,b (0.01 mol) and hydrazine hydrate (0.04 mol) in ethanol (100 mL) was refluxed for 6 h and cooled. Then a few drops of acetic acid were added. The produced solids were filtered off and recrystallized to give 9a,b respectively[41,42]. When the equimolar ratio of each of 9a,b and triethylphosphite in tetrahydrofuran (100 mL) and triethylamine (0.5 mL) was refluxed 6 h and cooled. The obtained solids were filtered off and recrystallized to give compounds 10a,b respectively. A mixture of 9a,b (0.01 mol) and triethylorthoformate (0.012 mol) in tetrahydrofuran (100 mL) was refluxed for 6 h and cooled. The produced solids were filtered off and recrystallized to give 11a,b respectively. The spectral data together with the physical constants of 11a,b are listed below. A mixture of 9a,b (0.01mole) and 2-chloro-6-fluorobenzeldehyde (0.01 mol) in ethanol (100 mL)/piperidine (0.5 mL) was refluxed 12 h and then cooled. The produced solids were filtered off and crystallized to give 12a,b respectively. A mixture of each of 9a,b (0.01 mol), CS2 (0.02 mol) in DMF (50 mL) was refluxed for 3 h and cooled, then poured onto the ice. The resulting solids so formed were filtered off and recrystallized to give 13a,b respectively. A mixture of each of 9a,b (0.01 mol) and ethyl chloroformate (0.012 mol) with tetrahydrofuran (100 mL) and triethylamine (0.5 mL, added dropwise) was refluxed for 3 h and then cooled. The solids obtained were filtered off and recrystallized to give 14a,b respectively.

The class="Chemical">first comclass="Chemical">pound (1) was class="Chemical">preclass="Chemical">pared by direct class="Chemical">pan class="Chemical">hydrazinolysis of 4-pyridyl-CONHNHCS2K according to Reid et al. reported method[43].

Characterization of the newly synthesized samples

The spectral data together with the physical constants oclass="Chemical">f all oclass="Chemical">pan class="Chemical">f the previously prepared compounds were identified as follows:

To conclass="Chemical">firm the occurrence oclass="Chemical">pan class="Chemical">f the reaction, Fourier transformer infrared (FTIR) spectrometer (a Perkin Lemer Spectrum RXI FT-IR systems No. 55529) was used. FT-IR spectra within the wavenumber ranged from 4000–600 cm−1 were recorded at room temperature in ATR discs.

class="Chemical">1H/ class="Chemical">pan class="Chemical">13C-NMR Spectra were recorded in (DMSO-d6) at 400 MHz with a Bruker NMR Advance DPX 400 MH Spectrometer. TMS was used as an internal reference. Chemical shifts were supposed to be due to the presence of the solvent.

The melting points opan class="Chemical">f the synthesized comclass="Chemical">pounds were determined by using SMP (Stuart Scienticlass="Chemical">pan class="Chemical">fic melting point) (USA).

GCMS-Q 1000 Ex spectrometer was used to measure the mass spectra oclass="Chemical">f the class="Chemical">preclass="Chemical">pared materials. Shimadzu UV-visible 3101 PC Sclass="Chemical">pectroclass="Chemical">photometer was used to record the electronic absorclass="Chemical">ption sclass="Chemical">pectra oclass="Chemical">pan class="Chemical">f the synthesized materials in DMF.

Antimicrobial activity

A bacterial suspension having a density oclass="Chemical">f about 1 to 2 ×108 (Cclass="Chemical">pan class="Chemical">FU) colony-forming units/ mL was prepared by aging a single colony on an agar plate for 24 hours. The antibacterial activity of some newly synthesized compounds was investigated on Mueller-Hinton agar using Agar diffusion techniques at concentrations of 100, 50 and 25 μg/mL, respectively. Muller Hinton agar (MHA) plates were inoculated with test inoculum (E. coli, Pseudomonas aeruginosa and Staphylococcus aureus) of standard inoculum (0.5 McFarland). Nalidixic acid and Imipenem were used as a reference drug for bacteria. Briefly, MHA agar plates were inoculated with bacterial strains under sterile conditions, and disc (diameter = 8 mm) was loaded with 50 μL of the tested sample. The plate was incubated at 37 °C for 24 hours. Nalidixic acid and Imipenem were used as a reference drug for bacteria. After the incubation period, the antimicrobial agent usually diffuses into the agar and inhibits the germination and growth of the test microorganism, and then according to the Clinical Laboratory Standards (CLSI), the diameter of the growth-inhibiting zone was measured[44]. The MICs of the prepared compounds were determined by the agar dilution plate technique following the standard procedure of the Clinical and Laboratory Standards[44]. The MICs were calculated from the X-axis intercept of the linear graph between log (Inhibitory concentration) and the growth inhibitory zone area.

The class="Chemical">fungal strain was grown in 5 mL Sabourad class="Chemical">pan class="Disease">Glucose Broth (glucose: peptone; 40: 10) for 3–4 days to reach 105 CFU/mL cells. Fungal cultures (0.1 mL) were spread evenly on Sabourad dextrose agar plates. The plates were inoculated with fungal strains under sterile conditions, and disc (diameter = 8 mm) was loaded with 50 μL of the test sample. The plate was then incubated at 30 °C for 3–4 days and according to the Clinical Laboratory Standards (CLSI), the diameter of the growth-inhibiting zone was measured[44]. Nystatin was used as antifungal standard drug[45].

Anti-inflammatory activity of the newly synthesized compounds

class="Chemical">Carrageenan-induced class="Chemical">pan class="Disease">paw edema method was used to evaluate the anti-inflammatory effect of the selected prepared compounds[46,47]. Indomethacin drug as a suspension in 24 tweens 80 was used as the reference drug, using Winter et al. method[46,47]. The inhibition percentage of inflammation was calculated using the following equation:

Microanalyses (CHNS elemental) and anti-inclass="Chemical">flammatory activity oclass="Chemical">pan class="Chemical">f the prepared compounds were carried out in the pharmaceutical Microbiology Department, National Center for Radiation Research and Technology (NCRRT), Nasr City, Egypt.

Molecular docking

Based on the results oclass="Chemical">f antibacterial screening and the anti-inclass="Chemical">pan class="Chemical">flammatory assay, docking of the most active compound 5d and the positive control, Nalidixic acid, in case of antibacterial screening was performed with the binding site of DNA Gyrase (topoisomerase II) enzyme (PDB ID: 2XCT). Also, docking of compounds 10a,b, 12a,b and the positive control Indomethacin in case of anti-inflammatory effect was performed with the binding site of COX-2 (PDB ID: 1CX2). Docking was done to shed light on their potential binding modes and investigate their similarity to the standard ligand binding modes[48-50].

Molecular docking procedure

X-ray crystal structure oclass="Chemical">f DNA Gyrase (toclass="Chemical">poisomerase II) comclass="Chemical">plexed with a class="Chemical">pan class="Chemical">Ciprofloxacin was determined at 3.35 Å resolution and X-ray crystal structure of COX-2 complexed with a selective inhibitor, sc-558 at 3 Å resolution. All molecular modeling calculations and docking studies were carried out using ‘Molecular Operating Environment 2019.0101’ software (MOE of Chemical Computing Group Inc., on a Core i7 2.2 GHz workstation) running on a Windows 10 PC.

Preparation of the targets (DNA Gyrase (topoisomerase II) enzyme and COX-2)

The X-ray crystallographic structures oclass="Chemical">f DNA Gyrase (toclass="Chemical">poisomerase II) enzyme (PDB ID: 2XCT) and cyclo-class="Chemical">pan class="Chemical">oxygenase 2 (PDB ID: 1CX2) were downloaded from the protein data bank (http://www.rcsb.org/)[51]. The enzyme was prepared for docking study by removal of chain B, C and D of its dimmers, water molecules, and ligands that are not involved in the binding. The enzyme was then prepared using quick preparation protocol in MOE with default options.

Docking validation

To conclass="Chemical">firm whether the aclass="Chemical">pclass="Chemical">plied docking class="Chemical">protocol is valid or not, Re-docking oclass="Chemical">pan class="Chemical">f the co-crystallized ligand into the enzyme was done. Based on the binding mode and rmsd (root mean square deviation), the coordinates of the native ligand in the co-crystallized PDB file were compared with the coordinates of the greatest scoring docking pose of the native ligand. The docking validation results revealed a near-perfect alignment with the original ligand as attained from the resolved X-ray PDB file. In the case of antibacterial screening, docking validation was confirmed from the small root mean square deviation (rmsd = 0.3155) between the docked pose and the co-crystallized ligand (S (energy score) of −11.2658 kcal/mol). Docking validation was also demonstrated by the ability of the docking poses to restore the main interactions that occur between the co-crystallized ligand and the active site’s hot spots, Manganese (Mn) atom and DG 5 of DNA Gyrase enzyme. In case of anti-inflammatory effect, the re-docked ligand showed a small root mean square deviation value (rmsd = 0.0215) between the docked compound and the co-crystallized ligand (S of −15.569 kcal/mol), they also showed a high ability to repeat the main interactions that occur between the co-crystallized ligand and the active site’s hot spots, Arg513 and His90 of COX-II.

Active compounds preparation for docking

Active compounds prepaclass="Species">ration class="Chemical">pan class="Chemical">for docking was done as follows: firstly, Marvin Sketch was used to built up the 2D structures of the docked ligands and copied onto MOE. This step is followed by 3D protonation of the active compounds structure. Then the systemic search was used for the running of the conformational analysis and then the smallest energetic conformer was selected. An identical docking protocol was applied with the ligand.

Running of docking

Docking studies were perclass="Chemical">formed using DNA Gyrase (toclass="Chemical">poisomerase II) enzyme co-crystallized with the native ligand oclass="Chemical">pan class="Chemical">f protein data bank file ID: 2XCT (PDB ID: 2XCT) and also COX-II enzyme co-crystallized with the native ligand of protein data bank file ID: 1CX2 (PDB ID: 1CX2). Posing compounds 5d and Nalidixic acid, in case of antibacterial screening, and compounds 10a,b, 12a,b and the positive control Indomethacin, in case of anti-inflammatory effect, was scored by London dG scoring function, that was used for docking, and Triangle Matcher placement protocol. The docked compounds of the greatest scoring pose were documented. Interactions between ligand and receptor in the formed complexes were tested in both 3D and 2D styles.

Results and Discussion

The spectral data and physical constants of the newly synthesized compounds

pan class="Chemical">For comclass="Chemical">pound (3a) class="Chemical">pan class="Chemical">(N3-(5-chloropyrimidin-2-yl)-5-phenyl-4H-1,2,4-triazole-3,4-diamine):

Yield 68% crystal class="Chemical">from class="Chemical">pan class="Chemical">dioxan; mp 213–215 oC. Analysis calculated for C12H10ClN7 (287): C, 50.10; N, 34.08; H, 3.50; Cl, 12.32. The analyses found for the compound are: C, 50.16; H, 3.57; Cl, 12.30; N, 34.15). The UV spectrum gave [λmax (Log ε)]: 372 nm. IR characteristic peaks appear at (ν cm−1): 3449, 3318 (NH2), 3189 (NH), 1601, 1581(C=N). 1H NMR characteristic peaks appear at δ ppm: 5.87(s, (2H), NH2), 4.0(s, (1H), NH), 8.05(s, 2H, (CH)-pyrimidine), 7.41–8.28 (m, 5H, CH-benzene). 13C NMR characteristic peaks appear at δ ppm: 119.0 (C5 of pyrimidine, C-Cl), 126.50,129.20 (C2 and C3 of benzene), 131.1 (C4 of benzene), 130.6 (C1 of benzene), 151.1 (C5 of triazole), 157.2 (C2 of triazole), 167.9 (C2 of pyrimidine), 156.8 (C4 and C6 of pyrimidine). MS (Int.%): 287 (5.1, M+), 271(12.5, M+ - NH2), 174 (22.5, M+ - 4-chloropyrimidyl), 159 (34.8, M+ - 4-chloropyrimidyl-NH), 128 (15.1, 4-chloropyrimidyl-NH), 113 (12.2, 4-chloropyrimidyl).

pan class="Chemical">For comclass="Chemical">pound (3b) (class="Chemical">pan class="Chemical">N3-(5-chloropyrimidin-2-yl)-5-(pyridin-4-yl)-4H-1,2,4-triazole-3,4-diamine):

Yield 61% crystal class="Chemical">from class="Chemical">pan class="Chemical">dioxan; mp 222–225 oC. Analysis calculated for C11H9ClN8 (288): C, 45.76; H, 3.14; Cl, 12.28; N, 38.81. The analyses found for the compound are: C, 45.70; H, 3.11; Cl, 12.30; N, 38.83). The UV spectrum gave [λmax (Log ε)]: 374 nm. IR characteristic peaks appear at (ν cm−1): 3442, 3325 (NH2), 3200 (NH), 1601,1581(C=N). 1H NMR characteristic peaks appear at δ ppm: 5.77(s, (2H), NH2), 4.0(s, (1H), NH), 8.05(s, 2H, (CH)-pyrimidine), 8.75(d, (2H), (CH)-pyridine), 7.99(d, (2H), (CH)-pyridine). 13C NMR characteristic peaks appear at δ ppm: 119.0 (C5 of pyrimidine, C-Cl), 121.3,149.8 (C2 and C3 of pyridine), 134 (C4 of pyridine), 157.2 (C5 of triazole), 151.1 (C2 of triazole), 167.9 (C2 of pyrimidine), 156.8 (C4 and C6 of pyrimidine). MS (Int.%): 288 (11.7, M+), 272(8.4, M+ - NH2), 175 (18.9, M+ - 4-chloropyrimidyl), 160 (31.2, M+ - 4-chloropyrimidyl-NH), 128 (13.4, 4-chloropyrimidyl-NH), 113 (11.8, 4-chloropyrimidyl).

pan class="Chemical">For comclass="Chemical">pound class="Chemical">pan class="Chemical">(3c) (N3-(5-bromopyrimidin-2-yl)-5-phenyl-4H-1,2,4-triazole-3,4-diamine):

Yield 52% crystal class="Chemical">from class="Chemical">pan class="Chemical">dioxan; mp 288–291 oC. Analysis calculated for C12H10BrN7 (331): C, 43.39; H, 3.03; Br, 24.06; N, 29.52. The analyses found for the compound are: C, 43.40; H, 3.01; Br, 24.08; N, 29.55). The UV spectrum gave [λmax (Log ε)]: 370(0.80)nm. IR characteristic peaks appear at (ν cm−1): 3440, 3350 (NH2), 3195 (NH), 1600, 1578 (C=N). 1H NMR characteristic peaks appear at δ ppm: 5.71(s, (2H), NH2), 3.7(s, (1H), NH), 8.53(s, 2H, (CH)-pyrimidine), 7.04–8.25(m, 5H, CH-benzene). 13C NMR characteristic peaks appear at δ ppm: 110.7 (C5 of pyrimidine, C-Br), 127.5,129.2 (C2 and C3 of benzene), 131.1 (C4 of benzene), 130.6(C1 of benzene), 157.2 (C5 of triazole), 151.1 (C2 of triazole), 168.4 (C2 of pyrimidine), 159.1 (C4 and C6 of pyrimidine). MS (Int.%): 331 (9.3, M+), 316 (9.1, M+ - NH2), 177 (22.3, M+ - 4-bromopyrimidyl), 162 (31.2, M+ - 4-bromopyrimidyl-NH), 170 (42.1, 4-bromopyrimidyl-NH), 155 (15.2, 4-bromopyrimidyl).

pan class="Chemical">For comclass="Chemical">pound (3d) class="Chemical">pan class="Chemical">(N3-(5-bromopyrimidin-2-yl)-5-(pyridin-4-yl)-4H-1,2,4-triazole-3,4-diamine):

Yield 48% crystal class="Chemical">from class="Chemical">pan class="Chemical">dioxane; mp 257–259 oC. Analysis calculated for C11H9BrN8 (332): C, 39.66; H, 2.72; Br, 23.98; N, 33.63 C, 39.66; H, 2.72; Br, 23.98; N, 33.63. The analyses found for the compound are: C, 39.62; H, 2.75; Br, 24.00; N, 33.65). The UV spectrum gave [λmax (Log ε)]: 374(0.84) nm. IR characteristic peaks appear at (ν cm−1): 3452, 3367 (NH2), 3188 (NH), 1600,1568 (C=N). 1H NMR characteristic peaks appear at δ ppm: 5.68(s, (2H), NH2), 4.2(s, (1H), NH), 8.56(s, 2H, (CH)-pyrimidine), 8.82(d, (2H), (CH)-pyridine), 7.95(d, (2H), (CH)-pyridine). 13C NMR characteristic peaks appear at δ ppm: 110.7 (C5 of pyrimidine, C-Br), 121.3, 149.8 (C2 and C3 of pyridine), 134.0 (C4 of pyridine), 157.2 (C5 of triazole), 151.1 (C2 of triazole), 168.4 (C2 of pyrimidine), 159.1 (C4 and C6 of pyrimidine). MS (Int.%): 332 (13.8, M+), 317(11.1, M+ - NH2), 178 (19.8, M+ - 4-bromopyrimidyl), 163 (33.5, M+ - 4-bromopyrimidyl-NH), 170 (31.5, 4-bromopyrimidyl-NH).

class="Chemical">For comclass="Chemical">pound (5a) (8-(5-chloroclass="Chemical">pyrimidin-2-yl)-3,6,6-triclass="Chemical">pan class="Chemical">phenyl-5,6,7,8-tetrahydro-[1,2,4]triazolo-[3,4-f] [1-3,5]triazaphosphinin-6-ol):

Yield 56%, crystals class="Chemical">from class="Chemical">pan class="Chemical">dioxan; mp 277–279 oC. Analysis calculated for C25H21ClN7OP (501): C, 59.83; H, 4.22; Cl, 7.06; N, 19.53; P, 6.17. The analyses found for the compound are: C,59.80; H, 4.23; N,19.56; Cl, 7.08, P, 6.18. IR characteristic peaks appear at (ν cm−1): 3080(aromatic (CH)), 2925(aliphatic (CH)), 1650(P-OH), 1522(C=N). 1H NMR characteristic peaks appear at δ ppm: 2.8(s, 1H, OH), 2.0(s, (1H), NH), 3.1(s, (2H), CH2), 7.41–8.05(m, 15H, CH-benzene), 8.08(s, 2H, (CH)-pyrimidine). 13C NMR characteristic peaks appear at δ ppm: 119.0 (C5 of pyrimidine, C-Cl), 130.6 (C1 of benzene, C-triazole), 127.5 (C2 of benzene attached with triazole), 129.2 (C3 of benzene attached with triazole), 131.1 (C4 of benzene attached with triazole), 131.2 (C-P of benzene), 84 (N-C-P of triaziphosphine), 157.2 (C5 of triazole), 151.1 (C2 of triazole), 167.9 (C2 of pyrimidine), 156.6 (C4 and C6 of pyrimidine).

class="Chemical">For comclass="Chemical">pound (5b) (8-(5-chloroclass="Chemical">pyrimidin-2-yl)-3-(class="Chemical">pan class="Chemical">pyridine-4-yl)-6,6-diphenyl-5,6,7,8-tetrahydro[1,2,4]triazolo[3,4-f][1-3,5]triazaphosphinin-6-ol):

Yield 43%, crystals class="Chemical">from class="Chemical">pan class="Chemical">dioxan; mp 300–302 oC. Analysis calculated for C24H20ClN8OP (502):C, 57.32; H, 4.01; Cl, 7.05; N, 22.28; O, 3.18; P, 6.16. The analyses found for the compound are: C, 57.40; H, 3.98; N,22.30; Cl, 7.07, P, 6.18. IR characteristic peaks appear at (ν cm−1): 3078(aromatic (CH)), 2943(aliphatic (CH)), 1647(P-OH), 1518(C=N). 1H NMR characteristic peaks appear at δ ppm: 3.1(s, 1H, OH), 2.3(s, (1H), NH), 3.6(s, (2H), CH2), 7.35–8.09(m, 10H, CH-benzene), 8.21(s, 2H, (CH)-pyrimidine), 8.75(d, (2H), (CH)-pyridine), 7.99(d, (2H), (CH)-pyridine). 13C NMR characteristic peaks appear at δ ppm: 119.0 (C5 of pyrimidine, C-Cl), 134(C4 of pyridine), 121.3 (C3 and C5 of pyridine), 149.8 (C2 and C6 of pyridine), 131.2 (C-P of benzene), 131.2 (C2 and C6 of benzene), 128.7 (C3,4,5 of benzene), 84.3 (N-C-P of triaziphosphine), 157.2 (C2 of triazole), 151.1 (C5 of triazole), 167.9 (C2 of pyrimidine), 156.6 (C4 and C6 of pyrimidine).

class="Chemical">For comclass="Chemical">pound (5c) (8-(5-class="Chemical">pan class="Chemical">Bromopyrimidin-2-yl)-3,6,6-triphenyl-5,6,7,8-tetrahydro [1,2,4]triazolo[3,4-f] [1-3,5]triazaphosphinin-6-ol):

Yield 51%, crystals class="Chemical">from class="Chemical">pan class="Chemical">dioxan; mp 222–225 oC. Analysis calculated for C25H21BrN7OP (554): C, 54.96; H, 3.87; Br, 14.62; N, 17.95; O, 2.93; P, 5.67. The analyses found for the compound are: C, 54.94; H, 3.86; Br, 14.65; N, 18.00; O, 2.93; P, 5.70. IR characteristic peaks appear at (ν cm−1): 3073(aromatic (CH)), 2952(aliphatic (CH)), 1643(P-OH), 1519 (C=N). 1H NMR characteristic peaks appear at δ ppm: 3.3(s, 1H, OH), 2.4(s, (1H), NH), 3.6(s, (2H), CH2), 7.30–8.05(m, 15H, CH-benzene), 8.34(s, 2H, (CH)-pyrimidine). 13C NMR characteristic peaks appear at δ ppm: 110.7 (C-Br of pyrimidine), 130.6 (C1 of benzene, C-triazole), 127.5 (C2 and C6 of benzene attached with triazole), 129.2 (C3 and C5 of benzene attached with triazole), 131.1 (C4 of benzene attached with triazole), 131.2 (C-P of benzene), 84.3 (N-C-P of triaziphosphine), 157.2 (C5 of triazole), 151.1 (C2 of triazole), 168.4 (C2 of pyrimidine, C-N), 159.1 (C4 and C6 of pyrimidine).

class="Chemical">For comclass="Chemical">pound (5d) (8-(5-class="Chemical">pan class="Chemical">Bromopyrimidin-2-yl)-3-(4-pyridin-4-yl)-6,6-triphenyl-5,6,7,8-tetrahydro[1,2,4]triazolo[3,4-f][1-3,5]triazaphosphinin-6-ol):

Yield 39%, crystals class="Chemical">from class="Chemical">pan class="Chemical">dioxan; mp 267–269 oC. Analysis calculated for C24H20BrN8OP (554): C, 54.96; H, 3.87; Br, 14.62; N, 17.95; O, 2.93; P, 5.67. The analyses found for the compound are: C, 54.94; H, 3.86; Br, 14.65; N, 18.00; O, 2.93; P, 5.70. IR characteristic peaks appear at (ν cm−1): 3073(aromatic (CH)), 2952(aliphatic (CH)), 1643(P-OH), 1519 (C=N). 1H NMR characteristic peaks appear at δ ppm: 3.3(s, 1H, OH), 2.4(s, (1H), NH), 3.6(s, (2H), CH2), 7.30–8.05(m, 15H, CH-benzene), 8.34(s, 2H, (CH)-pyrimidine). 13C NMR characteristic peaks appear at δ ppm: 110.7 (C-Br of pyrimidine), 130.6 (C1 of benzene, C-triazole), 127.5 (C2 and C6 of benzene attached with triazole), 129.2 (C3 and C5 of benzene attached with triazole), 131.1 (C4 of benzene attached with triazole), 131.2 (C-P of benzene), 84.3 (N-C-P of triaziphosphine), 157.2 (C5 of triazole), 151.1 (C2 of triazole), 168.4 (C2 of pyrimidine, C-N), 159.1 (C4 and C6 of pyrimidine).

class="Chemical">For comclass="Chemical">pound (6a) (8-(5-chloroclass="Chemical">pyrimidin-2-yl)-6-class="Chemical">pan class="Chemical">phenyl-3-(pyridin-2-yl)-7,8-dihydro-[1,2,4]triazolo-[4,3-b] [1,2,4]triazine):

Yield 51%, crystals class="Chemical">from class="Chemical">pan class="Chemical">dioxane/diluted by methanol, mp 171–173oC. Analysis calculated for C19H13ClN8 (388): C, 58.69; H, 3.37; Cl, 9.12; N, 28.82. The analyses found for the compound are: C, 58.7; H, 3.35; N, 28.9; Cl, 9.15. IR characteristic peaks appear at (ν cm−1): 3079.20, 2966 cm−1 (aromatic & aliphatic (CH)), 1522(C=N). 1H NMR characteristic peaks appear at δ ppm: 3.13(s, (2H), CH2) triazine ring, 8.05(s, 2H, (CH)-pyrimidine), 7.52–7.94(m, 5H, CH-benzene), 8.38 (d, 1H, CH- pyridine), 8.59(d, 1H, CH-pyridine), 7.36(dd, 1H, CH-pyridine), 7.85(dd, 1H, CH-pyridine). 13C NMR characteristic peaks appear at δ ppm: 134.7 (C1 of benzene), 128.2 (C2,6 of benzene), 128.8 (C3 and C5 of benzene), 131.0 (C4 of benzene), 157.2 (C5 of triazole), 151.1 (C2 of triazole), 164.6 (C6 of Triazine), 62.0 (C5 of Triazine),156.8 (C4 and C6 of pyrimidine), 119 (pyrimidine C-Cl), 155 (C2 pyridine, C-N), 124.2 (C3 pyridine), 137.2 (C4 pyridine), 123.6 (C5 pyridine), 149.2 (C6 pyridine).

class="Chemical">For comclass="Chemical">pound (6b) (8-(5-chloroclass="Chemical">pyrimidin-2-yl)-6-class="Chemical">pan class="Chemical">phenyl-3-(pyrimidin-4-yl)-7,8-dihydro[1,2,4]triazolo-[4,3-b] [1,2,4]triazine):

Yield 41%, crystals class="Chemical">from class="Chemical">pan class="Chemical">dioxan/diluted by methanol, mp 188–191 oC. Analysis calculated for C18H12ClN9 (389): C, 55.46; H, 3.10; Cl, 9.10; N, 32.34. The analyses found for the compound are: C, 55.5; H, 3.08; N, 32.3; Cl, 9.2. IR characteristic peaks appear at (ν cm−1): 3078, 2959 cm−1 (aromatic & aliphatic (CH)), 1519 (C=N). 1H NMR characteristic peaks appear at δ ppm: 3.4(s, (2H), CH2, triazine ring), 8.08 (s, 2H, (CH)-pyrimidine), 7.52–7.94(m, 5H, CH-benzene), 8.29 (d, 1H, CH- pyrimidine), 9.36(s, 1H, CH- pyrimidine), 9.20(d, 1H, CH- pyrimidine). 13C NMR characteristic peaks appear at δ ppm: 134.0 (C1 of benzene), 128.2 (C2,6 of benzene), 128.8 (C3 and C5 of benzene), 131.0 (C4 of benzene), 157.2 (C5 of triazole), 151.1 (C2 of triazole), 164.6 (C6 of triazine), 62.0 (C5 of triazine),156.8 (C4 and C6 of pyrimidine), 119 (pyrimidine C-Cl), 167.9 (C2 pyrimidine, C-N), 164 (C4 non-substituted pyrimidine), 115.6 (C5 of non-substituted pyrimidine), 157.1 (C6 of non-substituted pyrimidine).

class="Chemical">For comclass="Chemical">pound (6c) (8-(5-class="Chemical">pan class="Chemical">bromopyrimidin-2-yl)-6-phenyl-3-(pyridin-2-yl)-7,8-dihydro-[1,2,4]triazolo-[4,3-b] [1,2,4]triazine):

Yield 36%, crystals class="Chemical">from class="Chemical">pan class="Chemical">dioxan/diluted by methanol, mp 157–160 oC. Analysis calculated for C19H13BrN8 (432): C, 52.67; H, 3.02; Br, 18.44; N, 25.86. The analyses found for the compound are: C, 52.7; H, 3.01; N, 25.9; Br, 18.5. IR characteristic peaks appear at (ν cm−1): 3089, 2977 cm−1 (aromatic & aliphatic (CH)), 1520 (C=N). 1H NMR characteristic peaks appear at δ ppm: 3.3 (s, (2H), CH2, triazine ring), 8.5 (s, 2H, (CH)-pyrimidine), 7.4–7.8 (m, 5H, CH-benzene), 8.8 (d, 1H, CH- pyridine), 8.9 (d, 1H, CH-pyridine), 7.6 (dd, 1H, CH-pyridine), 7.8 (dd, 1H, CH-pyridine). 13C NMR characteristic peaks appear at δ ppm: 134.0 (C1 of benzene), 128.2 (C2,6 of benzene), 128.8 (C3 and C5 of benzene), 131.0 (C4 of benzene), 157.2 (C5 of triazole), 151.1 (C2 of triazole), 164.6 (C6 of triazine), 62.0 (C5 of triazine),159.1 (C4 and C6 of pyrimidine), 110.7 (pyrimidine C-Br), 168.4 (C2 pyrimidine, C-N), 155 (C2 pyridine, C-N), 124.2 (C3 pyridine), 137.2 (C4 of pyridine), 123.5 (C5 of pyridine), 149.2 (C6 of pyridine).

class="Chemical">For comclass="Chemical">pound (6d) (8-(5-class="Chemical">pan class="Chemical">bromopyrimidin-2-yl)-6-phenyl-3-(pyrimidin-4-yl)-7,8-dihydro-[1,2,4]triazolo-[4,3-b][1,2,4]triazine):

Yield 49%, crystals class="Chemical">from class="Chemical">pan class="Chemical">dioxan/diluted by methanol, mp 189–191 oC. Analysis calculated for C18H12BrN9 (433): C, 49.79; H, 2.79; Br, 18.40; N, 29.03. The analyses found for the compound are: C, 49.7; H, 2.8; N, 29.1; Br, 18.4. IR characteristic peaks appear at (ν cm−1): 3078, 2959 cm−1 (aromatic & aliphatic (CH)), 1522 (C=N). 1H NMR characteristic peaks appear at δ ppm: 3.5 (s, (2H), CH2, triazine ring), 8.4 (s, 2H, (CH)-pyrimidine), 7.5–7.9 (m, 5H, CH-benzene), 8.17 (d, 1H, CH- pyrimidine), 9.36(s, 1H, CH- pyrimidine), 9.20(d, 1H, CH- pyrimidine). 13C NMR characteristic peaks appear at δ ppm: 134.0 (C1 of benzene), 128.2 (C2,6 of benzene), 128.8 (C3 and C5 of benzene), 131.0 (C4 of benzene), 157.2 (C5 of triazole), 151.1 (C2 of triazole), 164.6 (C6 of triazine), 62.0 (C5 of triazine),159.1 (C4 and C6 of pyrimidine), 110.7 (pyrimidine C-Br), 168.4 (C2 pyrimidine, C-N), 167.9 (C2 pyrimidine, C-N), 164 (C4 pyrimidine, C-N), 115.5 (C5 of non-substituted pyrimidine), 157.1 (C6 of non-substituted pyrimidine).

class="Chemical">For comclass="Chemical">pound (class="Chemical">pan class="Chemical">7a) (8-(5-chloropyrimidin-2-yl)-6-methyl-3-phenyl-[1,2,4]triazolo[4,3-b][1,2,4]triazin-7(8H)-one):

Yield 55% crystal class="Chemical">from class="Chemical">pan class="Chemical">ethanol; mp 300–303 oC. Analysis calculated for C15H10ClN7O (339): C, 53.03; H, 2.97; Cl, 10.44; N, 28.86. The analyses found for the compound are: C,53.02; H, 3.0; N, 28.9; Cl, 10.4. IR characteristic peaks appear at (ν cm−1): 3089, 2988 cm−1 (aromatic & aliphatic (CH)), 1725 (C=O), 1590 (C=N). 1H NMR characteristic peaks appear at δ ppm: 2.07(s, 3H, CH3), 8.05(s, 2H, (CH)-pyrimidine), 7.41–8.28(m, 5H, CH-benzene). MS (m/z, %): 339 (M+, 100.0%), 341 (M + 2, 32.4%), 225 (M + - 5-chloropyrimidine, 13.4%), 227 ((M + 2)-5-chloropyrimidine, 4.8%), 114 (5-chloropyrimidine, 32%), 116 (5-chloropyrimidine, 10.3%).

class="Chemical">For comclass="Chemical">pound (class="Chemical">pan class="Chemical">7b) (8-(5-chloropyrimidin-2-yl)-6-methyl-3-(pyridin-4-yl)-[1,2,4]triazolo[4,3-b][1,2,4]-triazin-7(8H)-one):

Yield 39% crystal class="Chemical">from class="Chemical">pan class="Chemical">ethanol; mp 289–292 oC. Analysis calculated for C14H9ClN8O (340): C, 49.35; H, 2.66; Cl, 10.41; N, 32.89. The analyses found for the compound are: C,49.4; H, 2.7; N, 32.9; Cl, 10.4. IR characteristic peaks appear at (ν cm−1): 3091, 2979 cm−1 (aromatic & aliphatic (CH)), 1728 (C=O), 1522 (C=N). 1H NMR characteristic peaks appear at δ ppm: 2.18 (s, 3H, CH3), 8.02 (s, 2H, (CH)-pyrimidine), 7.99(d, (2H), (CH)-pyridine), 8.75(d, (2H), (CH)-pyridine). MS (m/z, %): 340 (M+, 100.0%), 342 (M+ + 2, 32.4%), 226 (M + - 5-chloropyrimidine, 12.3%), 228 ((M + 2) - 5-chloropyrimidine, 4.1%), 114 (5-chloropyrimidine, 15.3%), 116 (5-chloropyrimidine, 5.1%).

class="Chemical">For comclass="Chemical">pound (class="Chemical">pan class="Chemical">7c) (8-(5-bromopyrimidin-2-yl)-6-methyl-3-(pyridin-4-yl)-[1,2,4]triazolo[4,3-b][1,2,4]-triazin-7(8H)-one):

Yield 42% crystal class="Chemical">from class="Chemical">pan class="Chemical">ethanol; mp 244–246 oC. Analysis calculated for C15H10BrN7O (383): C, 46.89; H, 2.62; Br, 20.80; N, 25.52. The analyses found for the compound are: C,47.0; H, 2.6; N, 25.5; Br, 20.8. IR characteristic peaks appear at (ν cm−1): 3077, 2987 cm−1 (aromatic & aliphatic (CH)), 1724 (C=O), 1520 (C=N). 1H NMR characteristic peaks appear at δ ppm: 2.23 (s, 3H, CH3), 8.78 (s, 2H, (CH)-pyrimidine), 7.41–8.22 (m, 5H, CH-benzene). MS (m/z, %): 383 (M+, 100.0%), 385 (M + 2, 33.4%), 225 (M+ - 5-bromopyrimidine, 9.6%), 227 ((M + 2) - 5-bromopyrimidine, 3.2%), 158 (5-bromopyrimidine, 21.3%), 160 (5-bromopyrimidine, 7.1%).

class="Chemical">For comclass="Chemical">pound (7d) (8-(5-chloroclass="Chemical">pyrimidin-2-yl)-3-class="Chemical">pan class="Chemical">phenyl-[1,2,4]triazolo[4,3-b][1,2,4]triazine-6,7(5H,8H)-dione):

Yield 36% crystal class="Chemical">from class="Chemical">pan class="Chemical">ethanol; mp 274–276 oC. Analysis calculated for C14H9BrN8O (384): C, 43.66; H, 2.36; Br, 20.74; N, 29.09. The analyses found for the compound are: C,43.7; H, 2.4; N, 29.1; Br, 20.7. IR characteristic peaks appear at (ν cm−1): 3079, 2977 cm−1 (aromatic & aliphatic (CH)), 1730 (C=O), 1518 (C=N). 1H NMR characteristic peaks appear at δ ppm: 2.17(s, 3H, CH3), 8.43(s, 2H, (CH)-pyrimidine), 7.95 (d, (2H), (CH)-pyridine), 8.78 (d, (2H), (CH)-pyridine). MS (m/z, %): 384 (M+, 100.0%), 386 (M + + 2, 33.2%), 226 (M + - 5-bromopyrimidine, 11.7%), 228 ((M + 2) - 5-bromopyrimidine, 3.8%), 159 (5-bromopyrimidine, 17.5%), 161 (5-bromopyrimidine, 5.8%).

class="Chemical">For comclass="Chemical">pound (class="Chemical">pan class="Chemical">8a) (8-(5-chloropyrimidin-2-yl)-3-(pyridin-4-yl)-[1,2,4]triazolo[4,3-b][1,2,4]triazine-6,7(5H,8H)-dione):

Yield 42% crystalized class="Chemical">from class="Chemical">pan class="Chemical">ethanol; mp 152–155 oC. Analysis calculated for C14H8ClN7O2 (341): C, 49.21; H, 2.36; Cl, 10.38; N, 28.69. The analyses found for the compound are: C, 49.2; H, 2.4; N, 28.7, Cl, 10.4. IR characteristic peaks appear at (ν cm−1): 3233 (NH), 3079 (aromatic (CH)), 1734, 1658 (2C=O), 1522 (C=N). 1H NMR characteristic peaks appear at δ ppm: 7.6 (s, (1H), NH), 8.05 (s, 2H, (CH)-pyrimidine), 7.03–8.28 (m, 5H, CH-benzene).

class="Chemical">For comclass="Chemical">pound (8b) (8-(5-class="Chemical">pan class="Chemical">bromopyrimidin-2-yl)-3-phenyl-[1,2,4]triazolo[4,3-b][1,2,4]triazine-6,7(5H,8H)-dione):

Yield 38% crystalized class="Chemical">from class="Chemical">pan class="Chemical">ethanol; mp 215–217 oC. Analysis calculated for C13H7ClN8O2 (342): C, 45.56; H, 2.06; Cl, 10.35; N, 32.70. The analyses found for the compound are: C, 45.6; H, 2.1; N, 32.7, Cl, 10.3. IR characteristic peaks appear at (ν cm−1): 3197 (NH), 1729, 1666 (C=O), 1520 (C=N). 1H NMR characteristic peaks appear at δ ppm: 7.9 (s, (1H), NH), 8.12 (s, 2H, (CH)-pyrimidine), 7.96 (d, (2H), (CH)-pyridine), 8.05 (d, (2H), (CH)-pyridine).

class="Chemical">For comclass="Chemical">pound (8c) (8-(5-class="Chemical">pan class="Chemical">bromopyrimidin-2-yl)-3-phenyl-[1,2,4]triazolo[4,3-b][1,2,4]triazine-6,7(5H,8H)-dione):

Yield 52% crystalized class="Chemical">from class="Chemical">pan class="Chemical">ethanol/acetic acid mixture; mp 197–199 oC. Analysis calculated for C14H8BrN7O2 (384): C, 43.54; H, 2.09; Br, 20.69; N, 25.39. The analyses found for the compound are: C, 43.5; H, 2.1; N, 25.4, Br, 20.7. IR characteristic peaks appear at (ν cm−1): 3188 (NH), 3080 (aromatic (CH)), 1731, 1656 (C=O), 1519 (C=N). 1H NMR characteristic peaks appear at δ ppm: 7.81 (s, (1H), NH), 8.42 (s, 2H, (CH)-pyrimidine), 7.45–8.06 (m, 5H, CH-benzene).

class="Chemical">For comclass="Chemical">pound (8d) (8-(5-class="Chemical">pan class="Chemical">bromopyrimidin-2-yl)-3-(pyridin-4-yl)-[1,2,4]triazolo[4,3-b][1,2,4]triazine-6,7(5H,8H)-dione):

Yield 57% crystalized class="Chemical">from diluted class="Chemical">pan class="Chemical">ethanol; mp 223–225 oC. Analysis calculated for C13H7BrN8O2 (385): C, 40.33; H, 1.82; Br, 20.64; N, 28.94. The analyses found for the compound are: C, 40.3; H, 1.8; N, 28.9, Br, 20.6. IR characteristic peaks appear at (ν cm−1): 3180 (NH), 3088 (aromatic (CH)), 1735, 1668 (C=O), 1520 (C=N). 1H NMR characteristic peaks appear at δ ppm: 7.82 (s, (1H), NH), 8.43 (s, 2H, (CH)-pyrimidine), 7.87 (d, (2H), (CH)-pyridine), 8.15 (d, (2H), (CH)-pyridine).

pan class="Chemical">For comclass="Chemical">pound (9a) (class="Chemical">pan class="Chemical">3-hydrazinyl-5-phenyl-4H-1,2,4-triazol-4-amine):

Yield 67% crystal class="Chemical">from class="Chemical">pan class="Chemical">ethanol; mp 155–157 oC. Analysis calculated for C8H10N6 (190): C, 50.52; H, 5.30; N, 44.18. The analyses found for the compound are: C, 50.5; H, 5.3; N, 44.2. UV [DMF, λ max nm (Log ε)]: 316 (2.4) nm. IR characteristic peaks appear at (ν cm−1): 3344, (NH2), 3174 (NH), 3076 (aromatic (CH)), 1602 (deformation NH2), 1522 (C=N). 1H NMR characteristic peaks appear at δ ppm: 4.11 (s, (2H), NH2 of hydrazine), 5.77 (s, (2H), NH2 of triazole), 7.41–8.08 (m, 5H, CH-benzene), 9.23 (s, 1H, NH of hydrazine).

pan class="Chemical">For comclass="Chemical">pound (9b)(class="Chemical">pan class="Chemical">3-hydrazinyl-5-(pyridin-4-yl)-4H-1,2,4-triazol-4-amine):

Yield 58% crystal class="Chemical">from class="Chemical">pan class="Chemical">ethanol; mp 187–189 oC. Analysis calculated for C7H9N7 (191): C, 43.97; H, 4.74; N, 51.28. The analyses found for the compound are: C, 44.0; H, 4.7; N, 51.3. UV [DMF, λ max nm (Log ε)]: 312 (2.1) nm. IR characteristic peaks appear at (ν cm−1): 3356, (NH2), 3181 (NH), 1600 (deformation NH2), 1518 (C=N). 1H NMR characteristic peaks appear at δ ppm: 4.23 (s, (2H), NH2 of hydrazine), 5.53 (s, (2H), NH2 of triazole), 7.83 (d, (2H), (CH)-pyridine), 8.87 (d, (2H), (CH)-pyridine), 9.76 (s, (1H), NH).

class="Chemical">For comclass="Chemical">pound (10a) (7-class="Chemical">pan class="Chemical">phenyl-3,4-dihydro-[1,2,4]triazolo[4,3-e][1-5] tetrazaphosphinine):

Yield 58% crystal class="Chemical">from class="Chemical">pan class="Chemical">THF; mp 267–269 oC. Analysis calculated for C8H7N6P (218): C, 44.04; H, 3.23; N, 38.52; P, 14.20. The analyses found for the compound are: C, 44.0; H, 3.3; N, 38.50; P, 14.2. IR characteristic peaks appear at (ν cm−1): 3123–2962 (broad, NH-NH), 1650 (P-NH), 1528 (C=N); 1H NMR characteristic peaks appear at δ ppm: 4.25 (s, 1H, NH-N), 6.32 (s, 1H, NH-P), 7.41–8.28 (m, 5H, CH-benzene). MS (m/z, %): 218 (M + ,4.34%), 194 (M+ - NH-NH, 100%), 163 (M+ - NH-NH-P, 44%), 148 (M+ - NH-NH-P-N, 2.21%).

pan class="Chemical">For comclass="Chemical">pound (10b) (7-(class="Chemical">pyridin-4-yl)-3,4-dihydro-[1,2,4]triazolo[4,3-e][1-5] tetrazaclass="Chemical">phosclass="Chemical">phinine):

Yield 44% crystal class="Chemical">from class="Chemical">pan class="Chemical">THF; mp 226–228 oC. Analysis calculated for C7H6N7P (219): C, 38.37; H, 2.76; N, 44.74; P, 14.13. The analyses found for the compound are: C, 38.4; H, 2.8; N, 44.8; P, 14.1. IR characteristic peaks appear at (ν cm−1): 3120–2965 (broad, NH-NH), 1643 (P-NH), 1522 (C=N). 1H NMR characteristic peaks appear at δ ppm: 4.31 (s, 1H, NH-N), 6.52 (s, 1H, NH-P), 7.94 (d, (2H), (CH)-pyridine), 8.79 (d, (2H), (CH)-pyridine). MS (m/z, %): 219 (M+,6.22%), 195 (M+ - NH-NH, 100%), 164 (M+ - NH-NH-P, 37%), 149 (M+ - NH-NH-P-N, 5.11%).

pan class="Chemical">For comclass="Chemical">pound (11a) (class="Chemical">pan class="Chemical">3-phenyl-1,7-dihydro-[1,2,4]triazolo[4,3-b][1,2,4,5]tetrazine):

Yield 37% crystallized class="Chemical">from class="Chemical">pan class="Chemical">dioxane; mp 252–254 oC. Analysis calculated for C9H8N6 (200): C, 53.99; H, 4.03; N, 41.98. The analyses found for the compound are: C, 54.0; H, 4.0; N, 42.0. IR characteristic peaks appear at (ν cm−1): 3184,3132 (NH, NH of tetrazine and triazole), 1521 (C=N). 1H NMR characteristic peaks appear at δ ppm: 3.41 (s, 1H, NH-tetrazine), 5.82 (s, br., 1H, NH-triazole), 7.02 (s, 1H, CH-tetrazine), 7.51–7.82 (m, 5H, CH-benzene).

pan class="Chemical">For comclass="Chemical">pound (11b) (class="Chemical">pan class="Chemical">3-(pyridin-4-yl)-1,7-dihydro-[1,2,4]triazolo[4,3-b][1,2,4,5]tetrazine):

Yield 41% crystallized class="Chemical">from class="Chemical">pan class="Chemical">dioxan; mp 271–273 oC. Analysis calculated for C8H7N7 (201): C, 47.76; H, 3.51; N, 48.73. The analyses found for the compound are: C, 47.7; H, 3.5; N, 48.7. IR characteristic peaks appear at (ν cm−1): 3188, 3129 (NH, NH of tetrazine and triazole), 1519 (C=N). 1H NMR characteristic peaks appear at δ ppm: 3.62 (s, br., 1H, NH-tetrazine), 6.21 (s, br., 1H, NH-triazole), 7.36 (s, 1H, CH-tetrazine), 7.90 (d, (2H), (CH)-pyridine), 8.63 (d, (2H), (CH)-pyridine).

pan class="Chemical">For comclass="Chemical">pound (12a) (class="Chemical">pan class="Chemical">6-(2-chloro-6-fluorophenyl)-3-phenyl-5,6,7,8-tetrahydro-[1,2,4]triazolo[4,3-b][1,2,4,5]tetrazine):

Yield 53% crystal class="Chemical">from class="Chemical">pan class="Chemical">dioxan; mp 234–236 oC. Analysis calculated for C15H12ClFN6 (330): C, 54.47; H, 3.66; Cl, 10.72; F, 5.74; N, 25.41. The analyses found for the compound are: C, 54.5; H, 3.7; N, 25.4; F, 5.7; Cl, 10.7. UV [DMF, λ max nm (Log ε)]: 265 (1.13) and 263 (1.03) nm. IR characteristic peaks appear at (ν cm−1): 3354,3169 and 3132 cm−1 (3NH), 3078 (aromatic (CH)), 1522 cm−1 (C=N). 1H NMR characteristic peaks appear at δ ppm: 2.31 (s, 1H, NH-tetrazine at 2-position), 4.32 (s, 1H, NH-tetrazine at 4-position), 5.04 (s, 1H, CH-tetrazine), 6.32 (s, 1H, NH-tetrazine at 5-position), 7.07–8.28 (m, 8H, CH-aromatic). 13C NMR characteristic peaks appear at δ ppm: 157.2 (C3 of triazole), 151.1 (C5 of triazole), 130.6 (C1 of non-substituted benzene), 127.5 (C2, C6 of non-substituted benzene), 129.2 (C3, C5 of non-substituted benzene), 131.1 (C4 of non-substituted benzene), 73.5 (C3 of tetrazine), 133.8 (C-Cl), 160.8 (C-F), 129.8 (C2 of substituted benzene), 124.2, 129.7, 113.4 (3 C of substituted benzene).

class="Chemical">For comclass="Chemical">pound (12b) (6-(2-chloro-6-class="Chemical">pan class="Chemical">fluorophenyl)-3-(pyridin-4-yl)-5,6,7,8-tetrahydro-[1,2,4]triazolo[4,3-b][1,2,4,5] tetrazine):

Yield 46% crystal class="Chemical">from class="Chemical">pan class="Chemical">dioxan; mp 256–258 oC. Analysis calculated for C14H11ClFN7 (331): C, 50.69; H, 3.34; Cl, 10.69; F, 5.73; N, 29.56. The analyses found for the compound are: C, 50.7; H, 3.4; N, 29.5; F, 5.7; Cl, 10.6. UV [DMF, λ max nm (Log ε)]: 263(1.11) and 261 (1.01) nm. IR characteristic peaks appear at (ν cm−1): 3343, 3154 and 3129 cm−1 (3NH), 3072 (aromatic (CH)), 1518 cm−1 (C=N). 1H NMR characteristic peaks appear at δ ppm: 2.42 (s, 1H, NH-tetrazine at 2-position), 4.51 (s, 1H, NH-tetrazine at 4-position), 5.32 (s, 1H, CH-tetrazine), 6.50 (s, 1H, NH-tetrazine at 5-position), 7.01–7.32 (m, 3H, CH-aromatic), 7.92 (d, (2H), (CH)-pyridine), 8.71 (d, (2H), (CH)-pyridine). 13C NMR characteristic peaks appear at δ ppm: 157.2 (C3 of triazole), 151.1 (C5 of triazole), 134.0 (C4 of pyridine), 121.3 (C3, C5 of pyridine), 149.8 (C2, C6 of pyridine), 73.5 (C3 of tetrazine), 133.8 (C-Cl), 160.8 (C-F), 129.8 (C2 of substituted benzene), 124.2, 129.7, 113.4 (3C of substituted benzene).

class="Chemical">For comclass="Chemical">pound (class="Chemical">pan class="Chemical">13a) (3-phenyl-7,8-dihydro-[1,2,4]triazolo[4,3-b][1,2,4,5]tetrazine-6(5H)-thione):

Yield 45% crystal class="Chemical">from class="Chemical">pan class="Chemical">THF; mp 276–278 oC. Analysis calculated for C9H8N6S (232): C, 46.54; H, 3.47; N, 36.18; S, 13.81. The analyses found for the compound are: C, 46.5; H, 3.5; N, 36.2; S, 13.8. UV [λmax (Log ε)]: 314 (2.1) nm. IR characteristic peaks appear at (ν cm−1): 3332, 3178 (NH-NH), 1528 (C=S). 1H NMR characteristic peaks appear at δ ppm: 3.8 (s, br., 1H, -NH- tetrazine at position 2), 5.3 (s, br., 1H, NH-tetrazine at position 4), 6.1 (s, br., 1H, NH-tetrazine at position 5), 7.52–8.07 (m, 5H of benzene). 13C NMR characteristic peaks appear at δ ppm: 157.2 (C3 of triazole), 151.1 (C5 of triazole), 182.1 (C3 of tetrazine), 130.6 (C1 of benzene), 127.5 (C2,6 benzene), 129 (C3,5 of benzene), 131.1 (C4 of benzene).

pan class="Chemical">For comclass="Chemical">pound (13b) (class="Chemical">pan class="Chemical">3-(pyridin-4-yl)-7,8-dihydro-[1,2,4]triazolo[4,3-b][1,2,4,5]tetrazine-6(5H)-thione):

Yield 51% crystal class="Chemical">from class="Chemical">pan class="Chemical">THF; mp 255–258 oC. Analysis calculated for C8H7N7S (233): C, 41.19; H, 3.02; N, 42.03; S, 13.75. The analyses found for the compound are: C, 41.2; H, 3.0; N, 42.0; S, 13.8. UV [λmax (Log ε)]: 312 (1.8) nm. IR characteristic peaks appear at (ν cm−1): 3325, 3182 (NH-NH), 1522 (C=S). 1H NMR characteristic peaks appear at δ ppm: 3.2 (s, br., 1H, -NH- tetrazine at position 2), 5.1 (s, br., 1H, NH-tetrazine at position 4), 5.9 (s, br., 1H, NH-tetrazine at position 5), 7.99 (d, 2H of pridine at C3, C5), 8.77 (d, 2H of pyridine at C2, C6). 13C NMR characteristic peaks appear at δ ppm: 157.2 (C3 of triazole), 151.1 (C5 of triazole), 182.1 (C3 of tetrazine), 134.0 (C4 of pyridine), 149.8 (C2,6 of pyridine), 121.3 (C3,5 of pyridine).

class="Chemical">For comclass="Chemical">pound (class="Chemical">pan class="Species">14a) (3-phenyl-7,8-dihydro-[1,2,4]triazolo[4,3-b][1,2,4,5]tetrazin-6(5H)-one):

Yield 43% crystal class="Chemical">from class="Chemical">pan class="Chemical">ethanol; mp 191–193 oC. Analysis calculated for C9H8N6O (216): C, 50.00; H, 3.73; N, 38.87. The analyses found for the compound are: C, 50.1; H, 3.7; N, 38; 9. UV [λ max (Log ε)]: 324 (1.51) nm. IR characteristic peaks appear at (ν cm−1): 3167 (NH-NH), 1643 (amidic CO), 1563 (C=N); 1H NMR characteristic peaks appear at δ ppm: 2.9 (s, br., 1H, NH- tetrazine at position 2), 4.8 (s, br., 1H, NH-tetrazine at position 4), 5.6 (s, br., 1H, NH-tetrazine at position 5), 7.87–8.08 (m, 5H of benzene). 13C NMR characteristic peaks appear at δ ppm: 157.2 (C3 of triazole), 151.1 (C5 of triazole), 152.4 (C3 of tetrazine), 130.6 (C1 of benzene), 127.5 (C2,6 of benzene), 129.2 (C3,5 of benzene), 131.1 (C4 of benzene). MS (m/z, %): 216 (M+, 4.7%), 188 (M+ - CO, 100%), 173 (M+ - CO-NH, 4.3%).

class="Chemical">For comclass="Chemical">pound (class="Chemical">pan class="Chemical">14b) (3-(pyridin-4-yl)-7,8-dihydro-[1,2,4]triazolo[4,3-b][1,2,4,5]tetrazin-6(5H)-one):

Yield 38% crystal class="Chemical">from class="Chemical">pan class="Chemical">ethanol; mp 170–173 oC. Analysis calculated for C8H7N7O (217): C, 44.24; H, 3.25; N, 45.14. The analyses found for the compound are: C, 44.2; H, 3.3; N, 45.1. UV [λ max (Log ε)]: 320 (1.21) nm. IR characteristic peaks appear at (ν cm−1): 3172 (NH-NH), 1651 (amidic CO), 1534 (C=N). 1H NMR characteristic peaks appear at δ ppm: 3.1 (s, br., 1H, -NH- tetrazine at position 2), 5.1 (s, br., 1H, NH-tetrazine at position 4), 5.9 (s, br., 1H, NH-tetrazine at position 5), 7.98 (d, 2H of pyridine at C2,6), 8.67 (d, 2H, of pyridine at C3,5). 13C NMR characteristic peaks appear at δ ppm: 157.2 (C3 of triazole), 151.1 (C5 of triazole), 152.4 (C3 of tetrazine), 134.0 (C4 of pyridine), 149.8 (C2,6 of pyridine), 121.3 (C3,5 of pyridine). MS (m/z, %): 217 (M+, 6.2%), 189 (M+ - CO, 100%), 174 (M+ - CO-NH, 3.7%).

Samples characterization

class="Chemical">From the above mentioned sclass="Chemical">pectral data and class="Chemical">physical constants oclass="Chemical">pan class="Chemical">f the newly synthesized compounds, we concluded that: the mercapto group in the 4-amino-5-phenyl-4H-1,2,4-triazole-3-thiol (1a) is simply displaced by the amino group (nucleophilic group) in heterocyclic primary amines such as 5-chloropyrimidin-2-amine (2a) in Ethanol-DMF mixture (1:1) under reflux to yield 4-amino-5-substituted amino-1,2,4-triazole derivative (3a). Structure of 3a was elucidated mainly from the disappearance of the mercapto group peak at 2600–2500 cm−1 in the IR spectrum, the new NH proton with NH2 proton of aminotriazole of compound 3a showed through the 1H NMR spectrum at δ 14.22 ppm 5.87 ppm respectively. Also, the UV absorption of 3a shows λ max at 372 nm while the λ max of 1a appears at 317 nm, which proves the structure of compound 3a (Fig. 1). Ring closure reactions[39] of compound 3a with diphenyl(chloromethyl)-phosphanoxide (4) under reflux in tetrahydrofuran (THF) produces 3,8-diaryl-4,5,6,7-tetrahydro-6-hydroxy-1,2,4-triazole[4,3-b][1-3,5]-phosphotriazine (5a) (Fig. 1). The presence of C-H, P-OH, P-N and NH, P-N, P-OH, and C-H functional groups peaks at 1522, 1650, 2925 and 3080 cm−1 in its IR spectrum were used to deduce the structure of 5a. This structure was confirmed also by 1H NMR spectrum which showed resonated signals at δ 2.8 (s, 1H, OH), 3.1 (s, (2H), CH2), 2.0 (s, 1H, N-H), 7.41–8.05 (m, 15H, CH-benzene) and 8.08 (s, 2H, (CH)-pyrimidine).

Figure 1

Synthesis of triazolotriazine derivatives 5–8.

Synthesis opan class="Chemical">f class="Chemical">pan class="Chemical">triazolotriazine derivatives 5–8.

Derivatives oclass="Chemical">f class="Chemical">pan class="Chemical">1,2,4-Triazolo[4,3-b]-1,2,4-triazine compounds (6a–8a) have been obtained from the hetero-cyclization of compound 3a with phenacyl bromide dissolved in ethanolic potassium hydroxide, pyruvic acid dissolved sodium hydroxide and diethyloxalate dissolved in DMF/THF under reflux, respectively (Fig. 1). The chemical structures of compounds 6a–8a were elucidated from both the spectral measurements and the elemental analyses. Infrared spectrum analysis of compound 6a showed representative bands at ν 3079 (cm−1) and 2966 (cm−1) for aromatic & aliphatic (CH) and band at 1522 for (C=N). While, representing bands of compound 7a were recorded at ν = 2978 (cm−1), 1480 (cm−1) for (stretching and bending vibration of CH3) and a band at 1725(cm−1) for (C=O). In addition to that compound 8a bands were recorded at ν 3233 (cm−1) for NH, 1734(cm−1) and 1658(cm−1) for (2C=O), C=N and substituted pyrimidine and aromatic rings bands appear at 1522(cm−1) and 850–730 (cm−1). Also, 4 amino-5-(4 pyridyl)-4 H-1,2,4-triazole-3-thiol compound (1b) reacted with 5-chloro-pyrimidine-2-amine (2b) in Ethanol/DMF mixture (1:1) under reflux to give 4-amino-5-substituted amino-1,2,4-triazole derivative 3b. Compound 3b in turn, undergo ring closure with each of diphenyl (chloromethyl) - phosphanoxide (4) in THF/TEA, phenacyl bromide in ethanolic KOH, pyruvic acid in NaOH, and/or diethyloxalate in THF/DMF to provide the compounds 5b to 8b, respectively. The chemical structures of compounds 5b-8b were confirmed by considering both spectral measurements and elemental analyses. In a similar way, under the same above-mentioned experimental conditions, the reaction of compound 1a,b with 5-bromopyrimidin-2-amine (2c) takes place to give 3c,d compounds respectively. The triazole-3,4-diamines 3c,d were cyclized with each of diphenyl(choro-methyl)phosphanoxide (4), phenacyl bromide, pyruvic acid, and diethyloxalate to give the corresponding compounds 5c,d–8c,d, respectively, The chemical structures of compounds5c,d–8c,d were elucidated by their spectral measurements and elemental analyses data.

The reported results oclass="Chemical">f the high class="Chemical">potency oclass="Chemical">pan class="Chemical">f 1,2,4-triazolo[1,2,4,5]tetrazines system as antimicrobial and anti-inflammatory agents were our motivation to synthesize several derivatives of these rings. Thus, it was found that refluxing compound 1a,b with hydrazine hydrate dissolved in ethanol gives the corresponding 5 hydrazine-4-amino-1,2,4-triazoles (9a,b) which was used as new synthons for the present study aiming to build new 1,2,4-triazolotetrazines. A hydrazino group is a stronger nucleophilic group and more basic if it is compared to the amino group in both compounds 9a,b, which enhances the cyclization firstly followed by the amino center (Fig. 2). The triazolo[4,3-e][1-5] tetrazaphosphinines (10a,b) were obtained from refluxing 9a,b with triethylphosphite in tetrahydrofuran (THF), respectively. Treating compound 9a,b with triethylorthoformate under the previously mentioned conditions, produced the corresponding 3-(pyridin-4-yl)- and/or 3-phenyl-1,7-dihydro-[1,2,4]triazolo[4,3-b][1,2,4,5]-tetrazine 11a and/or 11b, respectively. Cycloaddition reaction, in boiling ethanol with a few drops of piperidine, of compounds 9a,b with 2-chloro–6-fluorobenzeldehyde furnished the corresponding triazolotetrazine derivatives 12 a,b, respectively (Fig. 2).

Figure 2

Synthesis of triazolotetrazine derivatives 10–14.

Synthesis opan class="Chemical">f class="Chemical">pan class="Chemical">triazolotetrazine derivatives 10–14.

Structures oclass="Chemical">f comclass="Chemical">pounds 10a,b, 11a,b and 12a,b were elucidated by examining the data oclass="Chemical">pan class="Chemical">f IR spectra. The IR absorption bands at ν (cm−1): 3123–2962, 3120–2965 of (broad NH-NH), 1650, 1643 of (P-NH), 1528, 1522 of (C=N) for 10a, b, respectively. The IR absorption bands at ν (cm-1): 3184–3132, 3188–3129 of (NH, NH of tetrazine and triazole), 1521, 1519 (C=N) for 11a,b, respectively. The IR absorption bands at ν (cm−1): 3354–3132, 3343–3129 (3NH), 3078, 3072 (aromatic (CH)), 1522, 1518 (C=N) for 12a,b, respectively. The mass spectra of each of 10a and 10b gave molecular ion peaks at 218 and 219 which corresponded to the molecular weights of C8H7N6P and C7H6N7P of the assigned structures 10a,b, respectively.

UV spectral data oclass="Chemical">f both comclass="Chemical">pounds 3a,b and 12a,b exclass="Chemical">plains that the hetero-cyclization would inhibit the electronic transition and this caused the hyclass="Chemical">psochromic eclass="Chemical">pan class="Chemical">ffect “shift to the shorter wavelength”. Thus, λmax of 3a,b was 372 and 374 nm while that of 12a,b was 265 and 263 nm, respectively. 13C NMR (DMSO) of compound 12a showed the presence of thirteen different signals for thirteen different carbon atoms, which, for 12b, showed twelve signals for twelve different carbons. Finally, the compounds 1,2,4-trizolo[4,3 -b][1,2,4,5]tetrazine-5(4 H)-thiones/ones (13a,b and 14a,b) were synthesized from refluxing of 9a,b with CS2 in DMF and/or with ethyl chloroformate in THF/TEA, respectively (Fig. 2). Formation of 13a,b occurs in two steps, the first, by addition to S=C=S and the second step is the elimination of H2S, while the formation of compounds 14a,b was carried via esterification of 9a,b followed by elimination of one molecule of ethanol in each case[52]. The chemical structures of 13a,b, and 14a,b were characterized by their elemental and spectral analyses. Thus, the infrared spectra of compounds 14a,b showed absorption bands of NH, CONH at ν 3167, 3172 and 1643, 1651cm−1, respectively, while that of 13a,b showed the absorption bands at ν 3332, 3325 cm−1 of NH and 1528, 1522 cm−1 of (C=S). The 1H NMR spectra of 13a,b recorded signals of 3.8 (s, 1H, -NH- tetrazine at position 2), 5.3 (s, 1H, NH-tetrazine at position 4), 6.1 (s, 1H, NH-tetrazine at position 5) and 7.52–8.07 (m, 5H of benzene). 13C NMR gives us good evidence for the structure of 13a where it showed resonated signals at 157 (C3 -triazole), 151 (C5 -triazole), 182 (C3 -tetrazine) and 130, 131,129, 127 ppm of benzene carbons. Mass spectra of both compounds 14a,14b showed molecular ion peaks at 216 (4.7%) for 14a and at 217 (6.2%) for 14b, which confirmed their structures. In addition, 13C NMR data of 14a and 14b gave convincing evidence to their structures in which compound 14a revealed the presence of seven different carbon signals, while 14b showed six different carbon signals, which were in good agreement with the proposed structures (Fig. 2).

Biological activity

The diclass="Chemical">fclass="Chemical">pan class="Chemical">ferent biological activities of the synthesized compounds have been evaluated by studying their antibacterial activity against Pseudomonas aeruginosa and Escherichia coli as examples for Gram-negative bacteria and Staphylococcus aureus as an example for Gram-positive bacteria, in addition, the antifungal activity against Candida albicans using the technique reported by Barry et al.[53,54]. Dimethylformamide was used as a solvent. Nystatin was used as a reference drug for fungi while Nalidixic acid and Imipenem were used as reference drugs for bacteria. The diameter of the growth inhibition zone (DIZ) was presented in Tables 1, 2 and Figs. 3–6. The Minimum Inhibitory Concentrations (MIC) in antibacterial activity were presented in Table 1.

Table 1

The antibacterial activity for some of the newly prepared Compounds.

S. aureus				P.aeruginosa				E. coli				Bacteria
MIC	25	50	100	MIC	25	50	100	MIC	25	50	100	InhibitoryConcentrations
Growth inhibition Zone (mm)*												Compounds
75.7	—	—	21	49.6	—	8	28	38.6	—	17	28	5a
48.5	—	9	23	68	—	—	26	44.3	—	15	31	5b
49.6	—	5	20	49.7	—	8	33	32	—	20	29	5c
48.9	—	8	18	46.9	—	11	30	31.7	—	22	32	5d
68.2	—	—	19	47.9	—	10	22	38.3	—	15	24	8a
67.2	—	—	24	79.6	—	—	20	37.3	—	17	27	8b
46.9	—	10	22	43.3	—	11	19	26.6	—	19	24	8c
48.4	—	9	24	79.5	—	—	17	44.6	—	14	29	8d
68.7	—	—	21	49	—	6	25	42.6	—	15	28	9a
46.6	—	11	25	61.3	—	—	22	44.5	—	13	26	9b
75.1	—	—	23	75.4	—	—	21	45.5	—	13	28	10a
49.5	—	7	22	45	—	10	20	45.7	—	12	25	10b
66.5	—	—	22	43.8	—	9	14	25.7	—	14	29	11a
48.2	—	8	19	40.4	—	11	17	43.8	—	16	32	11b
75.3	—	—	14	44.4	—	11	20	44.6	—	14	29	12a
39.5	—	10	15	53.5	—	—	25	38.7	—	16	26	12b
61.7	—	—	19	49.3	—	8	23	30.3	—	16	22	13a
37.2	—	11	16	49	—	9	26	45.2	—	13	27	13b
73.6	—	—	13	47.7	—	10	25	44.5	—	12	24	14a
31.6	—	10	13	49.6	—	8	28	43.7	—	14	27	14b
23.3	6	26	31	45.3	—	10	20	—	—	—	—	Nalidixic acid
14.2	17	20	26	15.9	14	19	23	13.9	18	22	28	Imipenem

Table 2

The antifungal activity for some of the newly prepared Compounds.

C. albicans			Fungi
25	50	100	Inhibitory Concentrations
Growth inhibition Zone (mm)*			Compounds
—	—	14	5a
—	—	17	5b
—	—	16	5c
—	—	14	5d
—	—	13	8a
—	—	14	8b
—	—	16	8c
—	—	14	8d
—	—	12	9a
—	—	11	9b
—	—	13	10a
—	—	12	10b
—	—	12	11a
—	—	14	11b
—	—	12	12a
—	—	10	12b
—	—	11	13a
—	—	13	13b
—	—	12	14a
—	—	11	14b
—	—	40	Nystatin

• Synthesized compounds (Inhibitor) Concentrations: 100, 50 and 25 μg /mL.

Highly active: DIZ ≥ 12.

Moderately active: DIZ = 9–12.

Slightly active: DIZ = 6–9;

Not sensitive: DIZ < 6 mm.

Figure 3

The antibacterial activity comparison of some tested compounds with Nalidixic acid and Imipenem toward E. coli at different concentrations where: (A) 25 µg/mL (B) 50 µg/mL (C) 100 µg/mL.

Figure 6

The antifungal activity comparison of some tested compounds with Nystatin toward C. albicans at different concentrations where: (A) 25 µg/mL (B) 50 µg/mL (C) 100 µg/mL.

The antibacterial activity class="Chemical">for some oclass="Chemical">pan class="Chemical">f the newly prepared Compounds.

The anticlass="Chemical">fungal activity class="Chemical">pan class="Chemical">for some of the newly prepared Compounds.

• Synthesized compounds (Inhibitor) Concentpan class="Species">rations: 100, 50 and 25 μg /mL.

Highly active: pan class="Chemical">DIZ ≥ 12.

Modepan class="Species">rately active: class="Chemical">pan class="Chemical">DIZ = 9–12.

Slightly active: pan class="Chemical">DIZ = 6–9;

Not sensitive: pan class="Chemical">DIZ < 6 mm.

The antibacterial activity comparison oclass="Chemical">f some tested comclass="Chemical">pounds with class="Chemical">pan class="Chemical">Nalidixic acid and Imipenem toward E. coli at different concentrations where: (A) 25 µg/mL (B) 50 µg/mL (C) 100 µg/mL.

The antibacterial activity comparison oclass="Chemical">f some tested comclass="Chemical">pounds with class="Chemical">pan class="Chemical">Nalidixic acid and Imipenem toward P.aeruginosa at different concentrations where: (A) 25 µg/mL (B) 50 µg/mL (C) 100 µg/mL.

The antibacterial activity comparison oclass="Chemical">f some tested comclass="Chemical">pounds with class="Chemical">pan class="Chemical">Nalidixic acid and Imipenem toward S. aureus at different concentrations where: (A) 25 µg/mL (B) 50 µg/mL (C) 100 µg/mL.

The anticlass="Chemical">fungal activity comclass="Chemical">parison oclass="Chemical">pan class="Chemical">f some tested compounds with Nystatin toward C. albicans at different concentrations where: (A) 25 µg/mL (B) 50 µg/mL (C) 100 µg/mL.

The results in Table 1 show that the antibacterial activity oclass="Chemical">f the tested comclass="Chemical">pounds could be classiclass="Chemical">pan class="Chemical">fied to higher to moderate activity against the used bacteria E.coli, S. aureus and P. aeruginosa in comparison with Nalidixic acid at concentrations 100, 50 and 25 µg/mL. All the tested Compounds showed higher activity against E. coli bacteria at 100 µg/mL concentrations and higher to moderate activity at 50 µg/mL concentrations. For S. aureus bacteria, all the tested Compounds showed higher activity at 100 µg/mL concentrations. Compounds 5a, 5c, 8a, 8b, 9a, 10a, 11a, 12a, 13a and 14a were not sensitive toward S. aureus while compounds 5d, 10b and 11b were slightly active at 50 µg/mL concentrations. On the other hand, the remaining compounds showed moderate activity at 50 µg/mL concentrations. For P. aeruginosa bacteria, all the tested compounds showed higher activity at 100 µg/mL concentrations. Compounds 5b, 8b, 8d, 9b, 10a and 12b were not sensitive toward P. aeruginosa bacteria while compounds 5a, 5c, 9a, 13a and 14b were slightly active at 50 µg/mL concentrations. On the other hand, the remaining compounds showed moderate activity at 50 µg/mL concentrations. The MICs for all the tested compounds against E.coli, S. aureus, and P. aeruginosa were presented in Table 1. The data in Table 2 showed moderate to high antifungal activities of the tested compounds against C. albicans in comparison with Nystatin at 100 µg/mL concentration The MIC for all the tested compounds against C. albicans fungi was 100 µg/mL. The higher activity of some of the tested compounds was mainly due to containing chlorine, fluorine, bromine and phosphorus elements within the chemical structure of 1,2,4-triazine and 1,2,4,5-tetrazine[55]. On the other hand, the obtained results in Table 3 indicated that: Compounds 10a,b, 12a,b and 13b carrying phenyl and pyridine groups having both the chlorine and fluorine elements had good anti-inflammatory activity, in comparison with the standard anti-inflammatory drug used (Indomethacin). Compounds 10a,b, and 12a,b contained mainly triazole and tetrazine rings with the presence of both fluorine and phosphorus elements, incorporated with pyridyl moiety. The activity of the new materials depends on the existence of those new moieties which have a high biological activity[55]. The higher biological activity of the synthesized compounds was in a good agreement with the previously stated results in the field of fluorine and phosphorus-bearing nitrogen heterocyclic systems[9,56].

Table 3

The anti-inflammatory activity for some of the newly synthesized compounds.

Compound	Dose (mg /Kg)	Paw edema(g)* ± S.E.	% inhibition
9a	25 5	0.57 ± 0.05 0.61 ± 0.06	13.63 7.57
9b	25 5	0.52 ± 0.05 0.59 ± 0.06	21.21 10.60
10a	25 5	0.42 ± 0.05 0.48 ± 0.06	36.36 27.27
10b	25 5	0.45 ± 0.05 0.49 ± 0.06	31.81 25.75
12a	25 5	0.41 ± 0.03 0.48 ± 0.06	37.87 27.27
12b	25 5	0.43 ± 0.05 0.47 ± 0.06	34.84 28.78
13a	25 5	0.55 ± 0.05 0.64 ± 0.05	16.66 3.03
13b	25 5	0.40 ± 0.05 0.46 ± 0.06	39.39 30.30
Control	0	0.66 ± 0.05	0
Indomethacin	5	0.32 ± 0.02	51.51

*Significant difference from the control value at p < 0.05.

The anti-inclass="Chemical">flammatory activity class="Chemical">pan class="Chemical">for some of the newly synthesized compounds.

*Signiclass="Chemical">ficant diclass="Chemical">pan class="Chemical">fference from the control value at p < 0.05.

Molecular modeling studies (Structure-based drug design)

Table 4 and class="Chemical">Fig. 7 illustclass="Chemical">pan class="Species">rate the results of the bonding interactions for the docking of compound 5d and Nalidixic acid with amino acids of DNA Gyrase enzyme (PDB ID: 2XCT) active site. From these results, we found that the active compound 5d showed extra binding modes to DA13 and Arg 458 in addition to interaction with the essential binding sites Mn metal and DG5.

Table 4

Molecular modeling results for compound 5d and Nalidixic acid during docking in DNA Gyrase enzyme (PDB ID: 2XCT) active site.

Compound	COX-2 (PDB: 2XCT)
	AffinityKcal/mol	Distance (in Å) frommain residue		Functionalgroup	Interaction	2d caption(3d caption)
5d	−14.2589	1.62 3.95 4.76 3.93 3.73	Mn Arg458 DG5 DA13 DA13	Pyridine N Diazine ring Phenyl ring Triazole ring Triazole ring	Metal Bond pi-H pi-H pi-pi pi-pi	Fig. 7a (Fig. 7b)
Nalidixic acid	−10.0699	1.71 3.99 3.67	Mn DG5 DG5	Carbonyl group Phenyl ring Phenyl ring	Metal Bond pi-pi pi-pi	Fig. 7c (Fig. 7d)

Figure 7

Molecular docked model of compound 5d and Nalidixic acid with DNA Gyrase enzyme (the target is presented as thin sticks; the ligands are drawn as ball-and-stick). Images (a,c) represent the 2D docking styles for DNA Gyrase enzyme with compound 5d and Nalidixic acid, respectively. Images (b,d) represent the 3D docking styles for compound 5d and Nalidixic acid, respectively.

Molecular modeling results pan class="Chemical">for comclass="Chemical">pound 5d and class="Chemical">pan class="Chemical">Nalidixic acid during docking in DNA Gyrase enzyme (PDB ID: 2XCT) active site.

pan class="Chemical">Arg458 class="Chemical">pan class="Chemical">DG5

pan class="Chemical">DA13

pan class="Chemical">DA13

pan class="Chemical">Pyridine N

pan class="Chemical">Phenyl ring

pan class="Chemical">Triazole ring

pan class="Chemical">Triazole ring

pan class="Chemical">Metal Bond

pan class="Chemical">Fig. class="Chemical">pan class="Chemical">7a

(pan class="Chemical">Fig. class="Chemical">pan class="Chemical">7b)

pan class="Chemical">DG5

pan class="Chemical">DG5

pan class="Chemical">Carbonyl grouclass="Chemical">p

pan class="Chemical">Phenyl ring

pan class="Chemical">Phenyl ring

pan class="Chemical">Metal Bond

pan class="Chemical">Fig. class="Chemical">pan class="Chemical">7c

(pan class="Chemical">Fig. 7d)

Molecular docked model oclass="Chemical">f comclass="Chemical">pound 5d and class="Chemical">pan class="Chemical">Nalidixic acid with DNA Gyrase enzyme (the target is presented as thin sticks; the ligands are drawn as ball-and-stick). Images (a,c) represent the 2D docking styles for DNA Gyrase enzyme with compound 5d and Nalidixic acid, respectively. Images (b,d) represent the 3D docking styles for compound 5d and Nalidixic acid, respectively.

Table 5 and class="Chemical">Fig. 8 illustclass="Chemical">pan class="Species">rate the results and the bonding interactions of the docked compounds and Indomethacin, respectively, with active sites of COX-2 (PDB ID: 1CX2).

Table 5

Molecular modeling results for compounds and Indomethacin during docking in COX-2 (PDB ID: 1CX2) active site.

Compound	COX-2 (PDB: 1CX2)
	AffinityKcal/mol	Distance (in Å) frommain residue		Functionalgroup	Interaction	2d caption(3d caption)
10a	−17.8710	4.47	Arg513	Triazole ring	pi-cation	Fig. 8a (Fig. 8b)
10b	−16.1990	2.47 3.71	Tyr355 Ser353	Triazole N Pyridine ring	H-acceptor pi-H	Fig. 8c (Fig. 8d)
12a	−15.3810	3.23 3.80 3.56	Leu352 Val523His90	-NH- Phenyl ring Triazole ring	H-donor pi-H pi-pi	Fig. 8e (Fig. 8f)
12b	−16.2058	3.86 3.37	Tyr355 Ala527	-NH- Triazole ring	H-acceptor pi-H	Fig. 8g (Fig. 8h)
Indomethacin	−15.8796	2.70	Arg513	Carbonyl	H-acceptor	Fig. 8i (Fig. 8j)

Figure 8

Molecular docked model of compounds 10a,b, 12a,b and Indomethacin with COX–II enzyme (the target is presented as thin sticks; the ligands are drawn as ball-and-stick). Images (a,c,e,g,i) represent the 2D docking styles for COX–II enzyme with compounds 10a,b, 12a,b and Indomethacin, respectively. Images (b,d,f,h,j) represent the 3D docking styles for COX–II enzyme with compounds 10a,b, 12a,b and Indomethacin, respectively.

Molecular modeling results pan class="Chemical">for comclass="Chemical">pounds and class="Chemical">pan class="Chemical">Indomethacin during docking in COX-2 (PDB ID: 1CX2) active site.

pan class="Chemical">Fig. class="Chemical">pan class="Chemical">8a

(pan class="Chemical">Fig. 8b)

pan class="Chemical">Tyr355

pan class="Chemical">Ser353

pan class="Chemical">Triazole N

pan class="Chemical">Pyridine ring

pan class="Chemical">Fig. 8c

(pan class="Chemical">Fig. 8d)

pan class="Chemical">Leu352

pan class="Chemical">Val523class="Chemical">pan class="Chemical">His90

pan class="Chemical">Phenyl ring

pan class="Chemical">Triazole ring

pan class="Chemical">Fig. 8e

(pan class="Chemical">Fig. 8class="Chemical">pan class="Chemical">f)

pan class="Chemical">Tyr355

pan class="Chemical">Ala527

pan class="Chemical">Triazole ring

pan class="Chemical">Fig. 8g

(pan class="Chemical">Fig. class="Chemical">pan class="Chemical">8h)

pan class="Chemical">Fig. 8i

(pan class="Chemical">Fig. 8j)

Molecular docked model oclass="Chemical">f comclass="Chemical">pounds 10a,b, 12a,b and class="Chemical">pan class="Chemical">Indomethacin with COX–II enzyme (the target is presented as thin sticks; the ligands are drawn as ball-and-stick). Images (a,c,e,g,i) represent the 2D docking styles for COX–II enzyme with compounds 10a,b, 12a,b and Indomethacin, respectively. Images (b,d,f,h,j) represent the 3D docking styles for COX–II enzyme with compounds 10a,b, 12a,b and Indomethacin, respectively.

class="Chemical">From these results, it aclass="Chemical">pclass="Chemical">pears that, generally, the tested comclass="Chemical">pounds and class="Chemical">pan class="Chemical">Indomethacin showed a comparable binding pattern. Compound 10a showed well interaction with Arg513 residue. Compound 10b showed well interaction with Ser353 and Tyr355 which is going alongside with the screening results. Also, compound 12a showed three binding interactions to Leu352, His90 and Val523 amino acid. Finally, compound 12b binds to Ala527 and Tyr355 residues.

Conclusions

Some novel heterocyclic compounds 5a,b–class="Species">14a,b containing class="Chemical">pan class="Chemical">fluorine, chlorine, bromine and phosphorus elements were synthesized utilizing 3-substituted-4-amino-5-substituted amino-1,2,4-triazoles 3a-d and 3-substituted-4-amino-5-hydrazino-1,2,4-triazole 9a,b compounds as building units in the synthesizing process. These newly prepared compounds were fully characterized through the spectral and elemental analyses which were completely fit with the assigned structures. A number of the synthesized compounds were screened against gram-positive bacteria, gram-negative bacteria, and fungi, such as Streptococcus aureus, Escherichia coli, Pseudomonas aeruginosa, and Candida albicans. Most of the newly prepared compounds showed a high antibacterial, antifungal, and anti-inflammatory in comparing with the standard commercial antibiotics Imipenem and Nalidixic acid, Nystatin and Indomethacin, respectively. Compounds 10a,b, and 12a,b containing both chlorine and fluorine elements showed high inflammation activity.