Josman Dantas Palmeira1, Marisa Haenni2, Véronique Metayer2, Jean-Yves Madec2, Helena Maria Neto Ferreira3. 1. Microbiology - Biological Sciences Department, Faculty of Pharmacy, University of Porto, Porto, Portugal; UCIBIO - Research Unit on Applied Molecular Biosciences, REQUIMTE, Portugal. Electronic address: josmandantasp@gmail.com. 2. Unité Antibiorésistance et Virulence Bactériennes, Université de Lyon - Anses Laboratoire de Lyon, France. 3. Microbiology - Biological Sciences Department, Faculty of Pharmacy, University of Porto, Porto, Portugal; UCIBIO - Research Unit on Applied Molecular Biosciences, REQUIMTE, Portugal.
Abstract
INTRODUCTION: The prevalence of Extended-Spectrum Beta-Lactamase (ESBL)-producing Enterobacteriaceae is increasing worldwide and the Agri-Food sector acts as a reservoir of clinically relevant ESBL genes. Our study aimed at detecting and characterizing ESBL-producing Enterobacteriaceae responsible for intestinal colonization of Brazilian bovines. MATERIAL AND METHODS: ESBL-producing E. coli isolates were recovered from fecal samples of healthy cattle in Northwest Brazil. Antimicrobial susceptibility was determined by disk diffusion. Resistance and virulence genes were identified by PCR and amplicons were sequenced, clonality was assessed by PFGE and MLST, and plasmids were characterized by replicon typing, S1-PFGE and Southern blot hybridizations. Transferability of ESBL genes was assessed by conjugation assay. RESULTS: A total of 40 ESBL-producing E. coli were characterized, which originated from 34/191 animals (17.8 %) and 15/22 farms (68.2 %). The blaCTX-M-8 gene was the most frequent ESBL gene (62.5 %), followed by blaSHV-2a (20.0 %), blaCTX-M-2 (10.0 %), and blaCTX-M-15 (7.5 %). The blaCTX-M-8 gene was localized on the IncI1/pST113 plasmid in multiple E. coli sequence types across unrelated animals and farms. DISCUSSION: We report the first characterization and a high prevalence of ESBL-producing E. coli in the beef cattle sector in Brazil, which is mainly supported by the spread of an epidemic IncI1/pST113/blaCTX-M-8 plasmid. Since Brazil is one of the biggest beef meat exporters worldwide, the spread of this ESBL plasmid across other sectors, countries and continents should be considered with attention.
INTRODUCTION: The prevalence of Extended-Spectrum Beta-Lactamase (ESBL)-producing Enterobacteriaceae is increasing worldwide and the Agri-Food sector acts as a reservoir of clinically relevant ESBL genes. Our study aimed at detecting and characterizing ESBL-producing Enterobacteriaceae responsible for intestinal colonization of Brazilian bovines. MATERIAL AND METHODS: ESBL-producing E. coli isolates were recovered from fecal samples of healthy cattle in Northwest Brazil. Antimicrobial susceptibility was determined by disk diffusion. Resistance and virulence genes were identified by PCR and amplicons were sequenced, clonality was assessed by PFGE and MLST, and plasmids were characterized by replicon typing, S1-PFGE and Southern blot hybridizations. Transferability of ESBL genes was assessed by conjugation assay. RESULTS: A total of 40 ESBL-producing E. coli were characterized, which originated from 34/191 animals (17.8 %) and 15/22 farms (68.2 %). The blaCTX-M-8 gene was the most frequent ESBL gene (62.5 %), followed by blaSHV-2a (20.0 %), blaCTX-M-2 (10.0 %), and blaCTX-M-15 (7.5 %). The blaCTX-M-8 gene was localized on the IncI1/pST113 plasmid in multiple E. coli sequence types across unrelated animals and farms. DISCUSSION: We report the first characterization and a high prevalence of ESBL-producing E. coli in the beef cattle sector in Brazil, which is mainly supported by the spread of an epidemic IncI1/pST113/blaCTX-M-8 plasmid. Since Brazil is one of the biggest beef meat exporters worldwide, the spread of this ESBL plasmid across other sectors, countries and continents should be considered with attention.
Authors: Julio A Benavides; Marília Salgado-Caxito; Andrés Opazo-Capurro; Paulina González Muñoz; Ana Piñeiro; Macarena Otto Medina; Lina Rivas; Jose Munita; Javier Millán Journal: Antibiotics (Basel) Date: 2021-04-30
Authors: Kristina Nesporova; Adam Valcek; Costas Papagiannitsis; Iva Kutilova; Ivana Jamborova; Lenka Davidova-Gerzova; Ibrahim Bitar; Jaroslav Hrabak; Ivan Literak; Monika Dolejska Journal: Microorganisms Date: 2021-04-17
Authors: Christa Ewers; Anno de Jong; Ellen Prenger-Berninghoff; Farid El Garch; Ursula Leidner; Sumeet K Tiwari; Torsten Semmler Journal: Front Microbiol Date: 2021-04-01 Impact factor: 5.640
Authors: Carlos Bastidas-Caldes; Daniel Romero-Alvarez; Victor Valdez-Vélez; Roberto D Morales; Andrés Montalvo-Hernández; Cicero Gomes-Dias; Manuel Calvopiña Journal: Infect Drug Resist Date: 2022-09-30 Impact factor: 4.177
Authors: Timo Homeier-Bachmann; Stefan E Heiden; Phillip K Lübcke; Lisa Bachmann; Jürgen A Bohnert; Dirk Zimmermann; Katharina Schaufler Journal: Antibiotics (Basel) Date: 2021-05-12