| Literature DB >> 32270752 |
Hao Lu1,2,3, Weikang Wang1,2,3, Jianjun Zhang1,2,3, Hongxia Shao1,2,3, Luyuan Li1,2,3, Tuofan Li1,2,3, Quan Xie1,2,3, Zhimin Wan1,2,3, Aijian Qin1,2,3, Jianqiang Ye1,2,3.
Abstract
An outbreak of inclusion body hepatitis caused by fowl adenovirus serotype 8 (FAdV-8) has caused significant economic losses in the poultry industry worldwide. However, a rapid serology test kit specific to FAdV-8 is not available to date. We developed a fiber-based ELISA using the purified GST-fiber of FAdV-8 as coating antigen to measure antibodies against FAdV-8. Specificity analysis showed that our ELISA could react with sera against FAdV-7, -8a, and -8b, but not with sera against the other pathogens tested. Moreover, detection of positive sera with our ELISA had 83% and 94% agreement with an indirect immunofluorescence assay (IFA) and a commercial ELISA from BioChek, respectively. Our ELISA was also effective in the detection of antibodies against FAdV-8 in sera from both experimentally infected and clinically vaccinated chickens. Our FAdV-8 fiber-based ELISA can be a valuable tool to specifically and sensitively detect antibodies against FAdV-7 and/or -8 in infected or vaccinated chickens.Entities:
Keywords: antibody detection; fiber-based ELISA; fowl adenovirus; serotype 7; serotype 8
Mesh：
Substances：
Year: 2020 PMID： 32270752 PMCID： PMC7377603 DOI： 10.1177/1040638720913354
Source DB: PubMed Journal: J Vet Diagn Invest ISSN： 1040-6387 Impact factor: 1.279