Jian Yuan1,2, Wen Li1,2, Jinxiao Zhu3, Shuli Deng1,2, Xuejin Tao4. 1. Department of Conservative Dentistry and Endodontics, The Affiliated Stomatology Hospital, Zhejiang University School of Medicine Hangzhou, Zhejiang, China. 2. Key Laboratory of Oral Biomedical Research of Zhejiang Province, Zhejiang University School of Stomatology Hangzhou 310006, Zhejiang, China. 3. Department of Stomatology, Wuxi Children's Hospital Wuxi, Jiangsu, China. 4. Center of Stomatology, Tongji Hospital Affiliated to Tongji Medical College Hua Zhong University of Science and Technology Wuhan, Hubei, China.
Abstract
BACKGROUND: RECK, as a negative MMP regulator, is extensively expressed in normal cells but decreased in tumors. In OSCC, the relationship between RECK and MMPs and the potential prognostic impact remains unclear. In this research, for the first time, we investigated the expression of RECK associated with MMPs during OSCC carcinogenesis in a large sample and its association with 5-year survival rate. MATERIAL AND METHODS: Immunohistochemical SP technique was applied to study the expression of RECK and MMP-2 and MMP-9 in 108 cases of OSCC and 30 normal oral mucosae. Univariate and multivariate Cox regression analysis was utilized for disease-free survival and overall survival, and analyzed by Kaplan-Meier method regarding RECK expression in patients of OSCC. RESULTS: We found lower expression of RECK in OSCC was 51.85% (56/108) compared with 93.33% (28/30) in the control group. However, the higher expression of MMP-2 and MMP-9 was 74.07% (80/108) and 70.37% (76/108) in OSCC, respectively, compared with 20% (6/30) and 13.3% (4/30) in the control group. Furthermore, the decrease of RECK expression and the increase of MMP-2, and -9 expression were significantly correlated with the loss of histologic differentiation, the occurrence of lymphatic metastasis, and the increase of OSCC clinical stage (P<0.05). OSCC patients with a low level of RECK expression had a lower rate of 5-year survival. CONCLUSION: RECK may prevent metastasis and improve OSCC patients' prognosis through a RECK/MMP-2, and -9 imbalance. Furthermore, RECK is a prospective prognostic indicator and therapeutic target for cancer molecular targeting therapy. Low expression of RECK may be a significant negative prognostic predictor. IJCEP
BACKGROUND:RECK, as a negative MMP regulator, is extensively expressed in normal cells but decreased in tumors. In OSCC, the relationship between RECK and MMPs and the potential prognostic impact remains unclear. In this research, for the first time, we investigated the expression of RECK associated with MMPs during OSCC carcinogenesis in a large sample and its association with 5-year survival rate. MATERIAL AND METHODS: Immunohistochemical SP technique was applied to study the expression of RECK and MMP-2 and MMP-9 in 108 cases of OSCC and 30 normal oral mucosae. Univariate and multivariate Cox regression analysis was utilized for disease-free survival and overall survival, and analyzed by Kaplan-Meier method regarding RECK expression in patients of OSCC. RESULTS: We found lower expression of RECK in OSCC was 51.85% (56/108) compared with 93.33% (28/30) in the control group. However, the higher expression of MMP-2 and MMP-9 was 74.07% (80/108) and 70.37% (76/108) in OSCC, respectively, compared with 20% (6/30) and 13.3% (4/30) in the control group. Furthermore, the decrease of RECK expression and the increase of MMP-2, and -9 expression were significantly correlated with the loss of histologic differentiation, the occurrence of lymphatic metastasis, and the increase of OSCC clinical stage (P<0.05). OSCC patients with a low level of RECK expression had a lower rate of 5-year survival. CONCLUSION:RECK may prevent metastasis and improve OSCC patients' prognosis through a RECK/MMP-2, and -9 imbalance. Furthermore, RECK is a prospective prognostic indicator and therapeutic target for cancer molecular targeting therapy. Low expression of RECK may be a significant negative prognostic predictor. IJCEP
Authors: Ha Neul Lee; Mithun Mitra; Oye Bosompra; David C Corney; Elizabeth L Johnson; Nadine Rashed; Linda D Ho; Hilary A Coller Journal: Mol Biol Cell Date: 2018-06-06 Impact factor: 3.612