Literature DB >> 3226906

A new method for the unidirectional deletion of DNA with Bal 31 nuclease using 2'-O-MeRNA-DNA chimeric adaptors.

S Mukai1, S Shibahara, H Morisawa.   

Abstract

We have developed a new procedure for unidirectional DNA deletion with Bal 31 nuclease. This method is based on the use of chimeric adaptors containing double strands of 2'-O-methyl RNA to block against an attack of Bal 31 nuclease. This contributes a wide application to learn the relation of structure-function of genes.

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Year:  1988        PMID： 3226906

Source DB:  PubMed          Journal:  Nucleic Acids Symp Ser        ISSN： 0261-3166


  4 in total

1.  Inhibition of human immunodeficiency virus (HIV-1) replication by synthetic oligo-RNA derivatives.

Authors:  S Shibahara; S Mukai; H Morisawa; H Nakashima; S Kobayashi; N Yamamoto
Journal:  Nucleic Acids Res       Date:  1989-01-11       Impact factor: 16.971

2.  2'-O-alkyl oligoribonucleotides as antisense probes.

Authors:  A M Iribarren; B S Sproat; P Neuner; I Sulston; U Ryder; A I Lamond
Journal:  Proc Natl Acad Sci U S A       Date:  1990-10       Impact factor: 11.205

3.  Highly efficient chemical synthesis of 2'-O-methyloligoribonucleotides and tetrabiotinylated derivatives; novel probes that are resistant to degradation by RNA or DNA specific nucleases.

Authors:  B S Sproat; A I Lamond; B Beijer; P Neuner; U Ryder
Journal:  Nucleic Acids Res       Date:  1989-05-11       Impact factor: 16.971

4.  New synthetic routes to protected purine 2'-O-methylriboside-3'-O-phosphoramidites using a novel alkylation procedure.

Authors:  B S Sproat; B Beijer; A Iribarren
Journal:  Nucleic Acids Res       Date:  1990-01-11       Impact factor: 16.971
  4 in total

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