Fei Zheng1, Xin Xin2, Fei He1, Jianyong Liu1, Yuechong Cui3. 1. Department of Obstetrics and Gynecology, Yiwu Maternity and Children Hospital, Jinhua, Zhejiang 322000, P.R. China. 2. Department of Obstetrics and Gynecology, Benxi Central Hospital, Benxi, Liaoning 117000, P.R. China. 3. Department of Human Health and Human Services, Yiwu Maternity and Children Hospital, Jinhua, Zhejiang 322000, P.R. China.
Intrauterine adhesions (IUA), also known as Asherman's syndrome, were first described in 1948 by Joseph G. Asherman (1). The causes of IUA include dilatation/curettage, postpartum hemorrhage, hysteromyomectomy, hysteroscopic operation and genital tuberculosis (2-4). The main clinical manifestations of IUAs are hypomenorrhea, recurrent abortion, amenorrhea, periodic hypogastralgia, infertility and altered placental implantation, which are conditions that can cause harm to patient health (5). Currently, the treatment for IUA is hysteroscopic adhesiolysis; however, there is still a high recurrence rate after this treatment method (6). A previous study reported re-adhesion rates of up to 62.5% after hysteroscopic adhesiolysis (6). Therefore, the prevention of IUA after intrauterine operations is particularly important.Hyaluronic acid is a highly polymerized chain of disaccharides and a mucopolysaccharide that has important physiological functions in the body, including lubrication of the joints, adjusting the permeability of blood vessel walls, regulating the spread and transport of proteins and electrolytes and promoting wound healing (7). Hyaluronic acid can bind with a large number of water molecules and improve tissue hydration, enhance cell resistance to mechanical injury and reduce post-traumatic granulation tissue and fibrous tissue formation (8,9). Due to its unique biocompatibility and enzymatic biodegradation, hyaluronic acid is often used to prevent postoperative tissue adhesion (8,9). At present, hyaluronic acid gel is widely used after intrauterine operation to prevent IUAs, and this therapy has achieved some beneficial effects (10); however, the rates of adhesion and pregnancy after an intrauterine operation are still unclear. The current paper reports a meta-analysis on the existing literature of clinical randomized controlled trials on the use of hyaluronic acid gel following intrauterine operations.
Materials and methods
Literature search
The Cochrane Library (https://www.cochranelibrary.com/), Embase (https://www.embase.com/) and PubMed (https://www.ncbi.nlm.nih.gov/pubmed/) databases were searched for articles published before July 31 2018, using the terms ‘hyaluronic acid’, ‘intrauterine adhesions’, ‘Asherman's syndrome’, ‘IUA’, ‘dilatation and curettage’, ‘abortion’ and ‘hysteroscopic’. No language limit was set on the original publication.
Study inclusion and exclusion criteria
The present study included randomized controlled clinical studies that used hyaluronic acid gel following an intrauterine operation. The subjects were selected if the women were considered to be of reproductive age (18-40 years), had undergone an intrauterine operation and if there were at least two treatment groups in the trial (with or without the use of hyaluronic acid gel) regardless of other assistive interventions, including the administration of estrogen or the use of oral contraceptives.Studies were excluded if they were case reports, articles without specific data or studies with clear defects in experimental design.
Evaluation index
The efficacy of hyaluronic acid gel interventions was evaluated using two outcome measures. The first was the incidence rate of IUAs, according to the grading method as described by the American Fertility Society in 1988(11) and the second was pregnancy rate. The outcome measures were assessed according to the following equation: Number of cases (IUA of pregnancy)/total number of cases.
Study quality evaluation and statistical analysis
The literature screening, bias risk assessment and data extraction were conducted systematically by two independent evaluators. Risk assessments were performed using the clinical randomized controlled trial evaluation method as described by the Cochrane reviewer's handbook version 5.1(12). All data were analyzed using the RevMan 5.3 software (Cochrane).The statistical heterogeneity of each study result was analyzed using the χ2 test and the statistical significance was set at a cut-off value of P=0.1. P<0.01 indicated statistical heterogeneity of the study. I2 was used to evaluate the heterogeneity of the results. An I2 score of <25% suggested that the heterogeneity may not be important; however, it indicated significant heterogeneity when it was >50%. If the I2 was >75%, a high degree of heterogeneity was indicated. When the I2 was >50%, a random-effects model was adopted followed by a subgroup and sensitivity analyses. When the I2 was <50%, a fixed-effects model was adopted.All subjects were included in the analysis and were divided into the treatment group, which received hyaluronic acid gel treatment after an intrauterine operation, or the control group, which did not receive treatment with hyaluronic acid gel after intrauterine operation. A dichotomous variable was expressed as the relative risk (RR) and 95% confidence interval (CI), while a continuous variable was expressed as the mean difference (MD).
Results
Baseline characteristics
A total of 285 studies were initially selected. A total of seven were included in the final analysis after screening (Fig. 1) (10,13-18). All 7 studies compared the efficacies of using hyaluronic acid gel as an adjuvant therapy (treatment group) against a control group (without the use of hyaluronic acid gel) after an intrauterine operation. A total of 952 women of reproductive age who underwent intrauterine operations were included. From these patients, 455 had been treated with hyaluronic acid gel postoperatively and 497 were controls. Among the 7 studies, 3 used common abortive procedures such as intrauterine operations (14,15,17) and 4 were performed using hysteroscopy (10,13,16,18). A total of six studies were performed using direct injection with the gel (10,16,18) and one used a temporary adhesion barrier, which hydrated into a gel within 24-48 h of administration into the patient (17). There were no statistically significant differences in baseline parameters between the treatment and control groups, as presented in Table I (10,13-18). The results of the quality assessment of the included studies are presented in Fig. 2. Allocation concealment methods for all the studies were not included.
Figure 1.
Flowchart depicting the method behind the selection of the studies.
Table I
Characteristics of randomized controlled trials.
First Author, year
Sample size (n)
Disease
Intervention
Gel(Type and dose)
Outcomes
(Refs.)
Fuchs et al, 2014
52
Retained Products of Conception
Gel and Hysteroscopy vs. Hysteroscopy
Oxiplex gel, 10 ml
IUAs, pregnancy
(10)
Acunzo et al, 2003
84
IUAs
Gel, oral antibiotics and hysteroscopic adhesiolysis vs. oral antibiotics and hysteroscopic adhesiolysis
Auto-crosslinked hyaluronic acid, 10 ml
IUAs
(13)
Hooker et al, 2017
149
Miscarriage of <14 weeks with at least one previous D&C
Gel and D&Cs vs. D&C
Auto-crosslinked hyaluronic acid, 10 ml
IUAs
(14)
Li et al, 2019
274
Delayed miscarriage without previous history of D&C
Gel and D&Cs vs. D&C
Auto-crosslinked hyaluronic acid, 3 ml
IUAs
(15)
Guida et al, 2004
132
Myomas, polyps, and uterine septa
Gel and hysteroscopic vs. hysteroscopic
Auto-crosslinked hyaluronic acid, 10 ml
IUAs
(16)
Tsapanos et al, 2002
150
Patients with incomplete, missed, or recurrent abortion scheduled for suction evacuation and curettage
Gel and D&C vs. D&C
Seprafilm™/12.7x15,2 cm
IUAs, pregnancy
(17)
Xiao et al, 2015
111
Moderate to severe IUA
Gel, Foley's catheter balloon and hysteroscopic adhesiolysis vs. Foley's catheter balloon and hysteroscopic adhesiolysis
Auto-crosslinked hyaluronic acid, 2 ml
IUAs
(18)
IUAs, intrauterine adhesions; D&C, dilatation and curettage.
Figure 2.
Biased risk assessment chart of studies included in the meta-analysis (n=7). Green indicates a low risk of bias, yellow indicates an unclear risk of bias and red indicates a high risk of bias.
Meta-analysis results
All seven studies reported the incidence rate of IUAs; however, only 4 reported the IUA score after the intrauterine operation (14-16,18). As indicated in Figs. 3 and 4, the hyaluronic acid gel reduced the incidence of IUA after an intrauterine operation (RR=0.42; 95% CI=0.30-0.57; P<0.001) and the IUA score after an intrauterine operation (MD=-1.29, 95% CI=-1.73 to -0.84, P<0.001). A subgroup analysis revealed that the preventive effect of hyaluronic acid gel on IUAs was not affected by the type of intrauterine operation, as presented in Fig. 5. Abortions had an RR of 0.40 with a 95% CI of 0.26-0.62 (P<0.001) and hysteroscopy had an RR of 0.44 with a 95% CI of 0.28-0.68 (P<0.001). The analysis also indicated that the preventive effect of hyaluronic acid gel on IUA was not significantly affected by primary diseases, namely abortion (RR=0.48; 95% CI=0.29-0.78; P=0.003), IUAs (RR=0.38; 95% CI=0.21-0.67; P<0.001), and submucosal myoma of uterus or endometrial polyps or mediastinum uterus (RR=0.40; 95% CI=0.18-0.90; P=0.03), as presented in Fig. 6. A total of two studies (10,17) reported the effect of the treatment on pregnancy rates and the results demonstrated that the hyaluronic acid gel could improve pregnancy rates after intrauterine operations (RR=1.94; 95% CI=1.46-2.6; P<0.001), as presented in Fig. 7.
Figure 3.
Forest plot of the incidence of intrauterine adhesions after intrauterine operation. CI, confidence interval; df, degrees of freedom; M-H, Mantel-Haenszel.
Figure 4.
Forest plot of the intrauterine adhesion score after an intrauterine operation. CI, confidence interval; df, degrees of freedom; IV, inverse variance.
Figure 5.
Forest plot of the incidence of intrauterine adhesions between abortion and hysteroscopy after an intrauterine operation. CI, confidence interval; df, degrees of freedom; M-H, Mantel-Haenszel.
Figure 6.
Forest plot of the incidence of postoperative intrauterine adhesion of different primary diseases after an intrauterine operation. CI, confidence interval; df, degrees of freedom; M-H, Mantel-Haenszel.
Figure 7.
Forest plot of pregnancy rate after an intrauterine operation. CI, confidence interval; df, degrees of freedom; M-H, Mantel-Haenszel.
Discussion
A total of seven studies were included in the present meta-analysis; all reported the incidence of IUA after intrauterine operation (10,13-18), but only four reported the IUA score (14-16,18). The results of the current study demonstrated that hyaluronic acid gel reduced IUA incidence and its score following an intrauterine operation. This reduction was not affected by the type of intrauterine operation or the presence of primary diseases. The effect of treatment on pregnancy rates was reported in two studies, and the results indicated that hyaluronic acid gel could improve pregnancy rates following an intrauterine operation (10,17). However, neither of these studies reported additional details such as the use of contraceptives or the number of live births after treatment. These aforementioned studies also did not provide sufficient follow-up data. In the present study, it was not possible to evaluate the effect of hyaluronic acid gel on postoperative pregnancy and live birth rates. None of the seven studies reported gel-related or surgical-related complications, including hemorrhage, perforation or cervical laceration.Hyaluronic acid is a highly polymerized chain of disaccharides and is a mucopolysaccharide. Due to its irregular coiled state, physical and chemical properties (19), it has important physiological functions in the body, such as lubricating the joints, adjusting the permeability of blood vessel walls, regulating the spread and transport of proteins and electrolytes and promoting wound healing (7). Due to its unique biocompatibility and enzymatic biodegradation, hyaluronic acid is often used to prevent postoperative tissue adhesion (8,9). However, its limited half-life causes it to degrade easily in the body, limiting its effectiveness (20). Hyaluronic acid has also been combined with other biopolymers, such as proteins or peptides, through esterification, cross-linking and other chemical modifications of hyaluronic acids (20). This has led to the production of related compounds, such as hyaluronic acid-carboxymethylcellulose gels, which are widely used to prevent tissue adhesion after surgery (20). In recent years, the preventive effect of hyaluronic acid gel on IUA has attracted wide attention. Hooker et al (14) randomly assigned 152 women to the auxiliary auto-crosslinked hyaluronic acid gel group (intervention group) and the non-assisted auto-crosslinked hyaluronic acid gel group (control group) following dilatation and curettage (D&C). A follow-up diagnostic hysteroscopy was conducted 8 to 12 weeks after the D&C; the results showed that the application of gel after D&C significantly reduced the incidence of postoperative IUAs. A further study using animal models supported these findings. Huberlant et al (21) performed bilateral uterine curettage in 20 New Zealand white rabbits where one uterine tube in each rabbit was its own control. The rabbits were randomized into two groups where the second uterine tube was treated with either hyaluronic acid or isotonic saline. The results suggested that the use of hyaluronic acid gel in animal models improved fertility and reduced the incidence of IUA.Previous studies have indicated that hormones, intrauterine balloons and intrauterine devices (IUD), achieved some curative effect in preventing IUAs, but there were no clear indicators as to the improvement of clinical symptoms and pregnancy rates of the patients (22-26). Abu Rafea et al (27) demonstrated that postoperative intrauterine placement of a Foley's catheter balloon exhibited no significant effect on childbirth and pregnancy (27). However, Gupta et al (28) revealed that a postoperative Foley's catheter balloon placement seemed to prevent IUA; however, this is yet to be validated in a study with a larger cohort. Although this method has had some success, the secondary tissue damage and secondary uplink infection caused by a Foley's balloon extraction remains to be determined. Krajčovičová et al (29) indicated that following hysteroscopic adhesiolysis, the probability of using a hyaluronic acid gel and an IUD to restore a normal menstrual cycle was 60.0 and 55.5% respectively, compared with the probability of the control group of 36.3%. The pregnancy rate of patients treated with the hyaluronic acid gel was 77.7%, significantly higher compared with patients treated with an IUD (60.0%). It can be inferred that the application of the hyaluronic acid gel is better than an IUD or balloon at improving the pregnancy rate after IUA separation. It was also demonstrated that hyaluronic acid can regulate inflammatory factors, promote the normal epithelialization and regeneration of wound tissues, effectively isolate adjacent healing tissues and prevent the formation of adhesions (30).In conclusion, the current study indicated that hyaluronic acid gels can significantly reduce IUA incidences after an intrauterine operation. Their efficacy does not appear to be affected by the type of intrauterine operation or the presence of primary diseases. The results of the current study also demonstrated that hyaluronic acid gels can significantly improve pregnancy rates following intrauterine operation. The seven studies included in the present analysis were similar in experimental design with no heterogeneity. In addition, the sample sizes were small and the follow-up times were short. These limitations may have affected the overall analysis. Therefore, a larger sample size with longer follow-up and appropriately designed randomized, controlled clinical studies are necessary to fully assess the efficacy of hyaluronic acid gel-assisted prevention of IUA following intrauterine operation. The results of the present study provide novel insight that may be used for the prevention of IUA.
Authors: Angelo B Hooker; Robert A de Leeuw; Jos W R Twisk; Hans A M Brölmann; Judith A F Huirne Journal: Hum Reprod Date: 2021-01-01 Impact factor: 6.918
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