Flavia V Ferreira1, Andreina M Herrmann-Andrade2, Carla D Calabrese2, Fernando Bello3, Daniel Vázquez3, Matías A Musumeci1,2. 1. Centro de Investigaciones y Transferencia de Entre Ríos, CONICET, Tavella, 1450, 3200, Concordia, Entre Ríos, Argentina. 2. Facultad de Ciencias de la Alimentación, Universidad Nacional de Entre Ríos, E3202BCJ, Concordia, Entre Ríos, Argentina. 3. Instituto Nacional de Tecnología Agropecuaria (INTA), Estación Experimental Agropecuaria Concordia, Estación Yuquerí s/n, 3200, Concordia, Entre Ríos, Argentina.
Abstract
AIMS: Penicillium digitatum, Alternaria alternata and Colletotrichum gloeosporiodies are pathogens responsible for large decays and production losses of citrus. They are commonly controlled by fungicides, whose excessive applications have led to the emergence of resistant P. digitatum strains. Alternative approaches are imperative for sustainable and environmental harmless citrus production, being biological control a promising strategy. The objective was to evaluate the potential of Trichoderma strains native from the rhizosphere of citrus trees to control these pathogens. METHODS AND RESULTS: Seven strains were isolated and identified as Trichoderma harzianum, T. ghizhouense, T. atroviride and T. koningiopsis through morphological and molecular analysis. Five of them showed effective antagonist performance in vitro against the pathogens. The strain T. harzianum IC-30 was the best biological control agent in vivo, obtaining a reduction of rot percentage around 80 % after three weeks of infection of oranges with P. digitatum A21 (resistant to pyrimethanil). This strain also showed the highest chitinase and glucanase activity. CONCLUSIONS: T. harzianum IC-30 is an optimal antagonist for the control of green mold spreading and other pathogens in post-harvest citrus fruits. SIGNIFICANCE AND IMPACT: The strain combined with supplementary practices could lead to sustainable management of citrus fungal diseases, dispensing with synthetic fungicides. This article is protected by copyright. All rights reserved.
AIMS: Penicillium digitatum, Alternaria alternata and Colletotrichum gloeosporiodies are pathogens responsible for large decays and production losses of citrus. They are commonly controlled by fungicides, whose excessive applications have led to the emergence of resistant P. digitatum strains. Alternative approaches are imperative for sustainable and environmental harmless citrus production, being biological control a promising strategy. The objective was to evaluate the potential of Trichoderma strains native from the rhizosphere of citrus trees to control these pathogens. METHODS AND RESULTS: Seven strains were isolated and identified as Trichoderma harzianum, T. ghizhouense, T. atroviride and T. koningiopsis through morphological and molecular analysis. Five of them showed effective antagonist performance in vitro against the pathogens. The strain T. harzianum IC-30 was the best biological control agent in vivo, obtaining a reduction of rot percentage around 80 % after three weeks of infection of oranges with P. digitatum A21 (resistant to pyrimethanil). This strain also showed the highest chitinase and glucanase activity. CONCLUSIONS:T. harzianum IC-30 is an optimal antagonist for the control of green mold spreading and other pathogens in post-harvest citrus fruits. SIGNIFICANCE AND IMPACT: The strain combined with supplementary practices could lead to sustainable management of citrus fungal diseases, dispensing with synthetic fungicides. This article is protected by copyright. All rights reserved.
Authors: Li Lu; Saowaluck Tibpromma; Samantha C Karunarathna; Ruvishika S Jayawardena; Saisamorn Lumyong; Jianchu Xu; Kevin D Hyde Journal: Pathogens Date: 2022-03-28
Authors: Larralde-Corona Claudia Patricia; Ramírez-González María Del Socorro; Rosas-Hernández Iván; De la Cruz-Arguijo Erika; De Santiago Carolina; Shirai Keiko; Narváez-Zapata José Alberto Journal: Biotechnol Rep (Amst) Date: 2021-06-22