Anna Wardowska1, Michał Komorniczak2, Aneta Skoniecka3, Barbara Bułło-Piontecka2, Katarzyna A Lisowska4, M Alicja Dębska-Ślizień2, Michał Pikuła3. 1. Laboratory of Tissue Engineering and Regenerative Medicine, Department of Embryology, Medical University of Gdansk, Gdansk, Poland. Electronic address: anna.wardowska@gumed.edu.pl. 2. Department of Nephrology, Transplantology and Internal Diseases, Medical University of Gdansk, Gdansk, Poland. 3. Laboratory of Tissue Engineering and Regenerative Medicine, Department of Embryology, Medical University of Gdansk, Gdansk, Poland. 4. Department of Pathophysiology, Medical University of Gdansk, Gdansk, Poland.
Abstract
OBJECTIVE: Systemic lupus erythematosus (SLE) is one of the autoimmune diseases, believed to be closely related to hyperactivity of B cells, overproduction of autoantibodies and immune complex formation and deposition in affected tissue. The autoreactive inflammation leads to multiorgan damage with kidney dysfunction in the forefront. Studies on lupus nephritis (LN), affecting the majority of SLE patients, are mainly focused on cells causing local inflammation. The aim of our work was to detect alterations in more accessible peripheral blood B cells in the course of SLE focusing on the influence of renal insufficiency (RI) on those parameters. METHODS: We performed a comprehensive flow cytometry analysis of B cell subpopulations, analyzed gene expression patterns with qPCR, and examined serum cytokine levels with multiplex cytokine/chemokine assay. RESULTS: We discovered distribution of specific B cell subsets, especially CD38+ cells, plasmablasts, associated with the presence and severity of the disease. Changes in expression of MBD2, DNMT1 and APRIL genes were not only associated with activity of SLE but also were significantly changed in patients with RI. CONCLUSIONS: All these results shed new light on the role of circulating B cells, their subpopulations, function, and activity in the SLE with kidney manifestation.
OBJECTIVE:Systemic lupus erythematosus (SLE) is one of the autoimmune diseases, believed to be closely related to hyperactivity of B cells, overproduction of autoantibodies and immune complex formation and deposition in affected tissue. The autoreactive inflammation leads to multiorgan damage with kidney dysfunction in the forefront. Studies on lupus nephritis (LN), affecting the majority of SLEpatients, are mainly focused on cells causing local inflammation. The aim of our work was to detect alterations in more accessible peripheral blood B cells in the course of SLE focusing on the influence of renal insufficiency (RI) on those parameters. METHODS: We performed a comprehensive flow cytometry analysis of B cell subpopulations, analyzed gene expression patterns with qPCR, and examined serum cytokine levels with multiplex cytokine/chemokine assay. RESULTS: We discovered distribution of specific B cell subsets, especially CD38+ cells, plasmablasts, associated with the presence and severity of the disease. Changes in expression of MBD2, DNMT1 and APRIL genes were not only associated with activity of SLE but also were significantly changed in patients with RI. CONCLUSIONS: All these results shed new light on the role of circulating B cells, their subpopulations, function, and activity in the SLE with kidney manifestation.
Authors: Víctor A Sosa-Hernández; Sandra Romero-Ramírez; Rodrigo Cervantes-Díaz; Daniel A Carrillo-Vázquez; Itze C Navarro-Hernandez; Laura P Whittall-García; Abdiel Absalón-Aguilar; Ana S Vargas-Castro; Raúl F Reyes-Huerta; Guillermo Juárez-Vega; David E Meza-Sánchez; Vianney Ortiz-Navarrete; Jiram Torres-Ruiz; Nancy R Mejía-Domínguez; Diana Gómez-Martín; José L Maravillas-Montero Journal: Front Immunol Date: 2022-05-19 Impact factor: 8.786