Mohammad Mirzakhani1, Mehdi Shahbazi2, Roghayeh Akbari3, Farshid Oliaei3, Masoumeh Asgharpour4, Hassan Nikoueinejad5, Mousa Mohammadnia-Afrouzi6. 1. Student Research Committee, School of Medicine, Babol University of Medical Sciences, Babol, Iran; Cellular and Molecular Biology Research Center, Health Research Institute, Babol University of Medical Sciences, Babol, Iran; Immunoregulation Research Center, Health Research Institute, Babol University of Medical Sciences, Babol, Iran. 2. Cellular and Molecular Biology Research Center, Health Research Institute, Babol University of Medical Sciences, Babol, Iran; Immunoregulation Research Center, Health Research Institute, Babol University of Medical Sciences, Babol, Iran. 3. Kidney Transplantation Center, Shahid Beheshti Hospital, Babol University of Medical Sciences, Babol, Iran. 4. Department of Nephrology, Rouhani Hospital, Babol University of Medical Sciences, Babol, Iran. 5. Department of Immunology, Baqiyatallah University of Medical Sciences, Tehran, Iran; Nephrology and Urology Research Center, Baqiyatallah University of Medical Sciences, Tehran, Iran. 6. Cellular and Molecular Biology Research Center, Health Research Institute, Babol University of Medical Sciences, Babol, Iran; Immunoregulation Research Center, Health Research Institute, Babol University of Medical Sciences, Babol, Iran. Electronic address: m.mohammadnia@mubabol.ac.ir.
Abstract
BACKGROUND: It was found that regulatory T cells (Tregs) importantly affect the maintenance of the kidney graft. However, Tregs are a heterogeneous population with less to more suppressive activity. The aim of this study was to determine the effects of different subsets of Tregs, as well as their ratio to effector T cells (Teff), on kidney transplantation outcomes. METHODS: A total of 58 participants were enrolled in this study and divided into four groups: (i) first kidney transplant recipients (stable 1); (ii) second kidney transplant recipients (stable 2); (iii) transplant recipients with acute rejection (AR); and (iv) healthy control subjects. By using flow cytometer, the frequencies of CD4+ CD25++ CD45RA- Foxp3hi activated Tregs (aTregs), CD4+ CD25+ CD45RA+ Foxp3lo resting Tregs (rTregs), CD4+ CD25+ CD45RA- Foxp3lo non-suppressive T cells, CD4+ CD25+ Foxp3- cells Teff, and total Tregs were analyzed in all subjects. RESULTS: The frequency of aTregs (as well as the ratio of aTregs/Tregs) was significantly lower in the AR patients than the other three groups. In contrast to AR patients, stables 1 and 2 had a higher aTreg/Treg ratio than those in the control group. Although patients with AR had a significantly lower total Tregs than the other three groups, the balance of total Tregs and Teff was similar between patients with and without AR. CONCLUSION: Patients with AR had poorer immunoregulatory properties than those with normal graft functioning, as well as those in the control group. These reduced immunoregulatory properties in patients with AR could lead to graft rejection.
BACKGROUND: It was found that regulatory T cells (Tregs) importantly affect the maintenance of the kidney graft. However, Tregs are a heterogeneous population with less to more suppressive activity. The aim of this study was to determine the effects of different subsets of Tregs, as well as their ratio to effector T cells (Teff), on kidney transplantation outcomes. METHODS: A total of 58 participants were enrolled in this study and divided into four groups: (i) first kidney transplant recipients (stable 1); (ii) second kidney transplant recipients (stable 2); (iii) transplant recipients with acute rejection (AR); and (iv) healthy control subjects. By using flow cytometer, the frequencies of CD4+ CD25++ CD45RA- Foxp3hi activated Tregs (aTregs), CD4+ CD25+ CD45RA+ Foxp3lo resting Tregs (rTregs), CD4+ CD25+ CD45RA- Foxp3lo non-suppressive T cells, CD4+ CD25+ Foxp3- cells Teff, and total Tregs were analyzed in all subjects. RESULTS: The frequency of aTregs (as well as the ratio of aTregs/Tregs) was significantly lower in the ARpatients than the other three groups. In contrast to ARpatients, stables 1 and 2 had a higher aTreg/Treg ratio than those in the control group. Although patients with AR had a significantly lower total Tregs than the other three groups, the balance of total Tregs and Teff was similar between patients with and without AR. CONCLUSION:Patients with AR had poorer immunoregulatory properties than those with normal graft functioning, as well as those in the control group. These reduced immunoregulatory properties in patients with AR could lead to graft rejection.
Authors: Abbas Shahi; Saeedeh Salehi; Shima Afzali; Ladan Gol Mohammad Pour Afrakoti; Marzie Esmaeili; Farzaneh Bagherpour; Ziba Aghsaeifard; Sanaz Dehghani; Gholamreza Pourmand; Ali Akbar Amirzargar Journal: Biomed Res Int Date: 2021-02-09 Impact factor: 3.411