Double crosslinked HLC-CCS hydrogel tissue engineering scaffold for skin wound healing.

Skin defects caused by various reasons are currently common clinical problems. At present, hydrogels have been proposed as tissue-engineered skin scaffolds to regenerate the tissues of the defect. We used human-like collagen (HLC), which was isolated and purified after high-density fermentation of recombinant E. coli BL21 The gel uses HLC and carboxymethylated chitosan (CCS) as raw materials and combines enzyme-chemical double cross-linking technology to form a three-dimensional porous network structure that mimics the human extracellular matrix, providing attachment points and nutrients for cell growth and proliferation. For comparison, we used a common hydrogel raw material, gelatin, to prepare a hydrogel in the same way. The experimental results show that the HLC-CCS skin scaffold hydrogel has good mechanical properties, high porosity and good histocompatibility. And full-thickness skin defect repair experiments show that this hydrogel has a good ability to promote skin tissue regeneration at the wound. In summary, this HLC-based double-crosslinked hydrogel can be used as a project strategy for skin defect repair.