| Literature DB >> 32239050 |
Samuel Lim1, Jiwoo Kim1, Yujin Kim1, Dawei Xu1, Douglas S Clark2.
Abstract
We describe a versatile CRISPR/Cas-based strategy to construct multi-enzyme complexes scaffolded on a DNA template in programmable patterns. Catalytically inactive dCas9 nuclease was used in combination with SpyCatcher-SpyTag chemistry to assemble enzymes in a highly modular fashion. Five enzymes comprising the violacein biosynthesis pathway were precisely organized in nanometer proximity; a notable increase in violacein production demonstrated the benefits of scaffolding.Entities:
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Year: 2020 PMID： 32239050 DOI： 10.1039/d0cc01174f
Source DB: PubMed Journal: Chem Commun (Camb) ISSN： 1359-7345 Impact factor: 6.222