Analyzing Protein Disorder with IUPred2A.

IUPred2A is a combined prediction tool designed to discover intrinsically disordered or conditionally disordered proteins and protein regions. Intrinsically disordered regions exist without a well-defined three-dimensional structure in isolation but carry out important biological functions. Over the years, various prediction methods have been developed to characterize disordered regions. The existence of disordered segments can also be dependent on different factors such as binding partners or environmental traits like pH or redox potential, and recognizing such regions represents additional computational challenges. In this article, we present detailed instructions on how to use IUPred2A, one of the most widely used tools for the prediction of disordered regions/proteins or conditionally disordered segments, and provide examples of how the predictions can be interpreted in different contexts.