Fátima Rodriguez1, Claudia Salinas2, Silvina Fernandez3, Sol Haim4, Marta Mollerach5, Wilma Basualdo6, Hector Castro7, Beatriz Quiñónez6, Rocio Arguello8, Monica Rodriguez9, Lorena Grau10, Carmen Espínola11, Gladys Velázquez12, Gloria Samudio13, Gloria Gomez14, Ana Campuzano15, Juana Ortellado16, Patricia Almada17, Rosa Guillén18. 1. Instituto de Investigaciones en Ciencias de la Salud, Universidad Nacional de Asunción. San Lorenzo, Paraguay. farrodriguez288@gmail.com. 2. Instituto de Investigaciones en Ciencias de la Salud, Universidad Nacional de Asunción. San Lorenzo, Paraguay. salinasdavalos@gmail.com. 3. Cátedra de Microbiología, Facultad de Farmacia y Bioquímica, Universidad de Buenos Aires, Buenos Aires, Argentina. silvinafernandez23@gmail.com. 4. Cátedra de Microbiología, Facultad de Farmacia y Bioquímica, Universidad de Buenos Aires, Buenos Aires, Argentina. solhaim@gmail.com. 5. Cátedra de Microbiología, Facultad de Farmacia y Bioquímica, Universidad de Buenos Aires, Buenos Aires, Argentina. martamollerach@gmail.com. 6. Hospital General Pediátrico Niños de Acosta Ñú, Ministerio de Salud Pública y Bienestar Social, San Lorenzo, Paraguay. 7. Hospital General Pediátrico Niños de Acosta Ñú, Ministerio de Salud Pública y Bienestar Social, San Lorenzo, Paraguay. castrohec@gmail.com. 8. Hospital General Pediátrico Niños de Acosta Ñú, Ministerio de Salud Pública y Bienestar Social, San Lorenzo, Paraguay. rochiarguello@yahoo.com.ar. 9. Instituto de Previsión Social. Asunción, Paraguay. morodrig@ips.gov.py. 10. Instituto de Previsión Social. Asunción, Paraguay. lorenagrau@gmail.com. 11. Instituto de Previsión Social. Asunción, Paraguay. mcespino@ips.gov.py. 12. Instituto de Previsión Social. Asunción, Paraguay. g-velazquez@hotmail.com. 13. Hospital Nacional de Itauguá, Ministerio de Salud Pública y Bienestar Social. Itauguá, Paraguay. gsamudio.samudio@gmail.com. 14. Hospital Nacional de Itauguá, Ministerio de Salud Pública y Bienestar Social. Itauguá, Paraguay. gloryegom@yahoo.com.mx. 15. Hospital de Clínicas, Facultad de Ciencias Médicas. Universidad Nacional de Asunción San Lorenzo, Paraguay. campuzanorolon@gmail.com. 16. Hospital de Clínicas, Facultad de Ciencias Médicas. Universidad Nacional de Asunción San Lorenzo, Paraguay. juaniorte@yahoo.com.mx. 17. Hospital de Clínicas, Facultad de Ciencias Médicas. Universidad Nacional de Asunción San Lorenzo, Paraguay. pattyal76@yahoo.com.ar. 18. Instituto de Investigaciones en Ciencias de la Salud, Universidad Nacional de Asunción. San Lorenzo, Paraguay. rmguillenf@gmail.com.
Abstract
INTRODUCTION: Staphylococcus aureus is considered one of the most important human pathogens, and its levels of resistance to methicillin have increased even in strains isolated from people without nosocomial risk factors. Molecular analysis is essential for understanding the patterns of dissemination. The objective of this study was to identify community-acquired methicillin-resistant S. aureus (CA-MRSA) clones that infected Paraguayan children patients in two periods of time. METHODOLOGY: An observational, descriptive study was designed to determine the genetic variability of 115 isolates of CA-MRSA recovered from children who attended four reference centers in Paraguay between 2009-2010 and 2012-2013. RESULTS: The combined use of Pulsed Field Gel Electrophoresis (PFGE), Multi-Locus Sequencing Typing, Multi-Locus Variable Analysis (MLVA) and Spa typing techniques allowed the identification of two dominant clones: ST30-IV-t019 (77%) and ST5-IV-t311 (10%), and the establishment of the former as the leading cause of CA-MRSA infections in children during the study period. CONCLUSIONS: This is the first study that provides epidemiological information as well as microbiological and molecular characteristics of CA-MRSA isolates recovered from children from Asunción and the Central Department of Paraguay. Copyright (c) 2020 Fatima Rodriguez, Claudia Salinas, Silvina Fernandez, Sol Haim, Marta Mollerach, Wilma Basualdo, Hector Castro, Beatriz Quinonez, Rocio Arguello, Monica Rodriguez, Lorena Grau, Carmen Espínola, Gladys Velazquez, Gloria Samudio, Gloria Gomez, Ana Campuzano, Juana Ortellado, Patricia Almada, Rosa Guillen.
INTRODUCTION:Staphylococcus aureus is considered one of the most important human pathogens, and its levels of resistance to methicillin have increased even in strains isolated from people without nosocomial risk factors. Molecular analysis is essential for understanding the patterns of dissemination. The objective of this study was to identify community-acquired methicillin-resistant S. aureus (CA-MRSA) clones that infected Paraguayan childrenpatients in two periods of time. METHODOLOGY: An observational, descriptive study was designed to determine the genetic variability of 115 isolates of CA-MRSA recovered from children who attended four reference centers in Paraguay between 2009-2010 and 2012-2013. RESULTS: The combined use of Pulsed Field Gel Electrophoresis (PFGE), Multi-Locus Sequencing Typing, Multi-Locus Variable Analysis (MLVA) and Spa typing techniques allowed the identification of two dominant clones: ST30-IV-t019 (77%) and ST5-IV-t311 (10%), and the establishment of the former as the leading cause of CA-MRSA infections in children during the study period. CONCLUSIONS: This is the first study that provides epidemiological information as well as microbiological and molecular characteristics of CA-MRSA isolates recovered from children from Asunción and the Central Department of Paraguay. Copyright (c) 2020 Fatima Rodriguez, Claudia Salinas, Silvina Fernandez, Sol Haim, Marta Mollerach, Wilma Basualdo, Hector Castro, Beatriz Quinonez, Rocio Arguello, Monica Rodriguez, Lorena Grau, Carmen Espínola, Gladys Velazquez, Gloria Samudio, Gloria Gomez, Ana Campuzano, Juana Ortellado, Patricia Almada, Rosa Guillen.
Authors: Ziad W Jaradat; Maysoon Khwaileh; Waseem Al Mousa; Qutaiba O Ababneh; Anas Al Nabulsi Journal: Pathog Glob Health Date: 2021-08-02 Impact factor: 3.735