Tanja K Eggersmann1, Philipp Baumeister2, Jorg Kumbrink3, Doris Mayr3, Elisa Schmoeckel3, Christian J Thaler1, Christian Dannecker1,4, Udo Jeschke1,4, Thomas Nagler5, Sven Mahner1, Kariem Sharaf6, Julia K S Gallwas1,7. 1. Department of Obstetrics and Gynecology, University Hospital, LMU Munich, Munich, Germany. 2. Department of Otorhinolaryngology, University Hospital, LMU Munich, Munich, Germany. 3. Institute of Pathology, Faculty of Medicine, LMU Munich, Munich, Germany. 4. Department for Gynecology and Obstetrics, University of Augsburg, Augsburg, Germany. 5. Mathematical Institute, Leiden University, Leiden, the Netherlands. 6. Department of Otorhinolaryngology, University Hospital, LMU Munich, Munich, Germany kariem.sharaf@med.uni-muenchen.de. 7. Department of Gynecology and Obstetrics, Georg-August-University Goettingen, Goettingen, Germany.
Abstract
BACKGROUND/AIM: The incidence of human papilloma virus (HPV)-related head and neck squamous cell carcinoma (HNSCC) has been increasing in the last decades. Analysis of oral brushing or rinsing samples for screening or stratification could potentially improve screening and prevention. PATIENTS AND METHODS: Oral brushes and mouthwashes were taken from 20 patients with HPV-associated HNSCC before definite therapy. HPV genotyping was performed for the detection of 14 high-risk HPV subtypes and correlated to DNA isolated from tumor tissue. RESULTS: Ten of 20 patients were tested HPV positive by using either method. There was a significant correlation between macroscopic visibility of tumor and positive HPV detection (p<0.001) and HPV detection and tumor size (p<0.001). HPV was detected in all macroscopically visible tumors. Half of the HPV cases who had macroscopically invisible tumors were missed by both methods. CONCLUSION: Both techniques are limited in the detection of macroscopically non-visible and small tumors. Therefore, the application of these techniques for screening or diagnosis of HNSCC is not recommended. Copyright
BACKGROUND/AIM: The incidence of human papilloma virus (HPV)-related head and neck squamous cell carcinoma (HNSCC) has been increasing in the last decades. Analysis of oral brushing or rinsing samples for screening or stratification could potentially improve screening and prevention. PATIENTS AND METHODS: Oral brushes and mouthwashes were taken from 20 patients with HPV-associated HNSCC before definite therapy. HPV genotyping was performed for the detection of 14 high-risk HPV subtypes and correlated to DNA isolated from tumor tissue. RESULTS: Ten of 20 patients were tested HPV positive by using either method. There was a significant correlation between macroscopic visibility of tumor and positive HPV detection (p<0.001) and HPV detection and tumor size (p<0.001). HPV was detected in all macroscopically visible tumors. Half of the HPV cases who had macroscopically invisible tumors were missed by both methods. CONCLUSION: Both techniques are limited in the detection of macroscopically non-visible and small tumors. Therefore, the application of these techniques for screening or diagnosis of HNSCC is not recommended. Copyright
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