Low-nitrogen tolerance comprehensive evaluation and physiological response to nitrogen stress in broomcorn millet (Panicum miliaceum L.) seedling.

Developing the new crop varieties with high productivity under low nitrogen (N) input is an important access to facilitate modern agricultural sustainability. In the present study, 20 broomcorn millet (Panicum miliaceum L.) varieties were characterized for their morphological and nutrient parameters to different low N levels in seedling. The results showed that 0.25 mM NH4NO3 was the standard concentration for the evaluation and identification of low-N tolerance. Through pearson's correlation analysis, principal component analysis, and subordinate function analysis, the tolerance of 20 varieties under N stress was evaluated and plant height, root length, shoot biomass, and shoot and root N content were considered as the evaluation system of low-N tolerance. Although leaves photosynthetic capacities and activities of N metabolism related enzymes showed the decreasing tendency to N stress, low-N tolerant varieties had higher activities in both leaves and roots as compared to low-N sensitive varieties. The work provides a reliable and comprehensive method for evaluating low-N tolerance in broomcorn millet and our data elucidate possible physiological adaptive mechanisms by which broomcorn millet tolerates N stress.