Alejandra Chaparro Padilla1, Laura Weber Aracena1, Ornella Realini Fuentes1, Daniela Albers Busquetts2, Marcela Hernández Ríos3, Valeria Ramírez Lobos4, Andrés Pascual la Rocca5, José Nart Molina5, Victor Beltrán Varas6, Stephanie Acuña-Gallardo7, Antonio Sanz Ruiz1. 1. Department of Periodontology, Centre for Biomedical Research, Faculty of Dentistry, Universidad de los Andes, Av. Plaza 2501, Las Condes 7620157, Santiago, Chile. 2. Department of Statistics, School of Dentistry, Faculty of Science, Universidad Mayor, Santiago, Chile. 3. Department of Pathology, Faculty of Dentistry, Universidad de Chile, Sergio Livingstone Pohlhammer 943, Independencia, 8380492, Santiago, Chile. 4. Department of Public Health and Epidemiology, Faculty of Dentistry, Universidad de los Andes, Santiago, Chile. 5. Deparment of Periodontology, School of Dentistry, Universitat Internacional de Catalunya, Josep Trueta s/n, 08194, Sant Cugat del Vallés, Barcelona, Spain. 6. Clinical Investigation and Dental Innovation Center (CIDIC), Dental School, Universidad de La Frontera, Temuco, Chile. 7. Department of Obstetrics and Gynaecology, Laboratory of Reproductive Biology, Faculty of Medicine, Universidad de los Andes, Santiago, Chile.
Abstract
OBJECTIVE: To characterize extracellular vesicles (EVs) in gingival crevicular fluid (GCF) and saliva samples from healthy/gingivitis and periodontitis patients and correlate them with clinical inflammatory periodontal parameters. MATERIAL AND METHOD: An exploratory study, including 86 subjects was conducted. Clinical and periodontal data were recorded and oral fluids samples were obtained. EVs were precipitated by ExoQuick-TC™ and characterized by nanoparticle tracking analysis (Nanosight™), western-blot (WB), transmission electron microscopy (TEM) and ELISA. RESULTS: TEM showed nanoparticles morphologically compatible with EVs and WB analysis revealed bands of specific EVs markers (CD9, TSG101, Alix) in both oral fluids of periodontitis and healthy/gingivitis subjects. The total concentration of EVs in GCF was increased in periodontitis patients compared to healthy/gingivitis subjects (p=0.017). However, we did not observe differences in the EVs concentration of saliva samples (p=0.190). The size of GCF-EVs was 144.2nm in periodontitis and 160.35nm in healthy/gingivitis patients (p=0.038). The CD63 exosome marker was increased in GCF of periodontitis patients (p=0.00001). The total concentration of EVs in GCF was correlated with bleeding on probing (rho=0.63, p=0.002), periodontal probing depth (rho=0.56, p=0.009) and clinical attachment level (rho=0.48, p=0.030). CONCLUSION: periodontitis patients have increased concentration of EVs in GCF and their role in periodontitis should be clarified. This article is protected by copyright. All rights reserved.
OBJECTIVE: To characterize extracellular vesicles (EVs) in gingival crevicular fluid (GCF) and saliva samples from healthy/gingivitis and periodontitispatients and correlate them with clinical inflammatory periodontal parameters. MATERIAL AND METHOD: An exploratory study, including 86 subjects was conducted. Clinical and periodontal data were recorded and oral fluids samples were obtained. EVs were precipitated by ExoQuick-TC™ and characterized by nanoparticle tracking analysis (Nanosight™), western-blot (WB), transmission electron microscopy (TEM) and ELISA. RESULTS: TEM showed nanoparticles morphologically compatible with EVs and WB analysis revealed bands of specific EVs markers (CD9, TSG101, Alix) in both oral fluids of periodontitis and healthy/gingivitis subjects. The total concentration of EVs in GCF was increased in periodontitispatients compared to healthy/gingivitis subjects (p=0.017). However, we did not observe differences in the EVs concentration of saliva samples (p=0.190). The size of GCF-EVs was 144.2nm in periodontitis and 160.35nm in healthy/gingivitispatients (p=0.038). The CD63 exosome marker was increased in GCF of periodontitispatients (p=0.00001). The total concentration of EVs in GCF was correlated with bleeding on probing (rho=0.63, p=0.002), periodontal probing depth (rho=0.56, p=0.009) and clinical attachment level (rho=0.48, p=0.030). CONCLUSION:periodontitispatients have increased concentration of EVs in GCF and their role in periodontitis should be clarified. This article is protected by copyright. All rights reserved.
Authors: Rafael Pedro Madeira; Lavínia Maria Dal'Mas Romera; Paula de Cássia Buck; Charles Mady; Barbara Maria Ianni; Ana Claudia Torrecilhas Journal: J Immunol Res Date: 2021-01-11 Impact factor: 4.818