Adrian Rodríguez-Carlos1, Valentin Trujillo2, Irma Gonzalez-Curiel3, Sara Marin-Luevano4, Flor Torres-Juarez4, Alan Santos-Mena4, Cesar Rivas-Santiago5, Jose A Enciso-Moreno4, Veronica Zaga-Clavellina6, Bruno Rivas-Santiago7. 1. Unidad de Investigación Médica-Zacatecas, Instituto Mexicano del Seguro Social, Zacatecas, México; División de Medicina Molecular y Traslacional, Centro de Investigación en Ciencias de la Salud y Biomedicina, Universidad Autónoma de San Luis Potosí, San Luis Potosí, México. 2. Unidad de Investigación Médica-Zacatecas, Instituto Mexicano del Seguro Social, Zacatecas, México; Atopy (Allergy) Research Center, Juntendo University Graduate School of Medicine, Hongo, Bunkyo-ku, Tokyo, Japan. 3. Facultad de Química, Universidad Autónoma de Zacatecas, Zacatecas, México. 4. Unidad de Investigación Médica-Zacatecas, Instituto Mexicano del Seguro Social, Zacatecas, México. 5. Consejo Nacional de Ciencia y Tecnología, Catedras-CONACyT, Mexico; Biological Sciences School, Universidad Autónoma de Zacatecas, Zacatecas, México. 6. Rama de Inmunobioquímica, Instituto Nacional de Perinatologia, Ciudad México, México. 7. Unidad de Investigación Médica-Zacatecas, Instituto Mexicano del Seguro Social, Zacatecas, México. Electronic address: rondo_vm@yahoo.com.
Abstract
BACKGROUND: Diabetic foot ulcers (DFUs) are one of the main complications in patients with type 2 diabetes mellitus (DM2), previous studies have reported that DM2 patients have lower production of host defense peptides (HDP). AIM OF THE STUDY: To investigate the expression of RNase 7, cathelicidin, HBD-2, and psoriasin in biopsies obtained from DM2 patients with or without DFU. METHODS: Biopsies from DFU patients grade 3 according to Wagner's classification, from diabetic patients without ulcer and from healthy donors were obtained. qPCR, immunohistochemistry and cell line cultures were performed. To assess whether L-isoleucine, calcitriol, phenyl butyrate, metformin, glyburide or insulin induced RNase 7, keratinocytes were stimulated, and RNase 7 expression was evaluated. RESULTS: Our data showed that RNase 7 levels were decreased in both diabetic groups when were compared with skin from healthy donors. Since most of the DM2 patients are treated with drugs to reduce glycemia, we investigated whether glyburide, metformin or insulin were able to induce any change regarding RNase 7 production. Results showed that metformin reduces the expression of RNase 7 in in vitro treated keratinocytes, suggesting that the chronic use of metformin should be evaluated in DFU patients, whereas calcitriol, phenyl butyrate and L-isoleucine did not increase the RNase 7 production. CONCLUSIONS: Due RNase 7 has antimicrobial activity, its downregulation can make prone to DM2 patients to develop infections and impaired wound healing.
BACKGROUND:Diabetic foot ulcers (DFUs) are one of the main complications in patients with type 2 diabetes mellitus (DM2), previous studies have reported that DM2 patients have lower production of host defense peptides (HDP). AIM OF THE STUDY: To investigate the expression of RNase 7, cathelicidin, HBD-2, and psoriasin in biopsies obtained from DM2 patients with or without DFU. METHODS: Biopsies from DFU patients grade 3 according to Wagner's classification, from diabeticpatients without ulcer and from healthy donors were obtained. qPCR, immunohistochemistry and cell line cultures were performed. To assess whether L-isoleucine, calcitriol, phenyl butyrate, metformin, glyburide or insulin induced RNase 7, keratinocytes were stimulated, and RNase 7 expression was evaluated. RESULTS: Our data showed that RNase 7 levels were decreased in both diabetic groups when were compared with skin from healthy donors. Since most of the DM2 patients are treated with drugs to reduce glycemia, we investigated whether glyburide, metformin or insulin were able to induce any change regarding RNase 7 production. Results showed that metformin reduces the expression of RNase 7 in in vitro treated keratinocytes, suggesting that the chronic use of metformin should be evaluated in DFU patients, whereas calcitriol, phenyl butyrate and L-isoleucine did not increase the RNase 7 production. CONCLUSIONS: Due RNase 7 has antimicrobial activity, its downregulation can make prone to DM2 patients to develop infections and impaired wound healing.
Authors: Moritz Blumer; Tom Brown; Mariella Bontempo Freitas; Ana Luiza Destro; Juraci A Oliveira; Ariadna E Morales; Tilman Schell; Carola Greve; Martin Pippel; David Jebb; Nikolai Hecker; Alexis-Walid Ahmed; Bogdan M Kirilenko; Maddy Foote; Axel Janke; Burton K Lim; Michael Hiller Journal: Sci Adv Date: 2022-03-25 Impact factor: 14.136