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Literature DB >> 322281

Five hundredfold overproduction of DNA ligase after induction of a hybrid lambda lysogen constructed in vitro.

S M Panasenko, J R Cameron, R W Davis, I R Lehman.

Abstract

A lambda vector that contains the gene for Escherichia coli DNA ligase (lambdagt4-lop-11 lig+) has been modified to achieve overproduction of this enzyme. The third Eco RI site in the lambda chromosome has been altered by mutation, and the left-hand Eco RI fragment has been shortened. The new vector, lambdagt4-lop-11 lig+, forms a stable lysogen which, upon induction, produces a 100-fold increase in DNA ligase activity. Introduction of a phage mutation (S7) that prevents cell lysis results in an even greater increase (500-fold).

Entities: Gene Species

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Year: 1977 PMID： 322281 DOI： 10.1126/science.322281

Source DB: PubMed Journal: Science ISSN： 0036-8075 Impact factor: 47.728

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Cited

42 in total

1. Role of the RNA polymerase alpha subunits in MetR-dependent activation of metE and metH: important residues in the C-terminal domain and orientation requirements within RNA polymerase.

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2. The quorum sensing negative regulators EsaR and ExpR(Ecc), homologues within the LuxR family, retain the ability to function as activators of transcription.

Authors: Susanne B von Bodman; Jessica K Ball; Marie A Faini; Carmen M Herrera; Timothy D Minogue; Mark L Urbanowski; Ann M Stevens
Journal: J Bacteriol Date: 2003-12 Impact factor: 3.490

3. Characterization of lambdapolA transducing phages; effective expression of the E. coli polA gene.

Authors: N E Murray; W S Kelley
Journal: Mol Gen Genet Date: 1979-08

4. Gene for the RNA polymerase sigma subunit mapped in Salmonella typhimurium and Escherichia coli by cloning and deletion.

Authors: J G Scaife; J S Heilig; L Rowen; R Calendar
Journal: Proc Natl Acad Sci U S A Date: 1979-12 Impact factor: 11.205

9. DNA sequence and characterization of GcvA, a LysR family regulatory protein for the Escherichia coli glycine cleavage enzyme system.

Authors: R L Wilson; G V Stauffer
Journal: J Bacteriol Date: 1994-05 Impact factor: 3.490

10. Penetration of human intestinal epithelial cells by Salmonella: molecular cloning and expression of Salmonella typhi invasion determinants in Escherichia coli.

Authors: E A Elsinghorst; L S Baron; D J Kopecko
Journal: Proc Natl Acad Sci U S A Date: 1989-07 Impact factor: 11.205

Five hundredfold overproduction of DNA ligase after induction of a hybrid lambda lysogen constructed in vitro.

1. Role of the RNA polymerase alpha subunits in MetR-dependent activation of metE and metH: important residues in the C-terminal domain and orientation requirements within RNA polymerase.

2. The quorum sensing negative regulators EsaR and ExpR(Ecc), homologues within the LuxR family, retain the ability to function as activators of transcription.

3. Characterization of lambdapolA transducing phages; effective expression of the E. coli polA gene.

4. Gene for the RNA polymerase sigma subunit mapped in Salmonella typhimurium and Escherichia coli by cloning and deletion.

5. Regulation of the Salmonella typhimurium metA gene by the metR protein and homocysteine.

6. Selection for mutations in the PR promoter of bacteriophage lambda.

7. Nucleotide sequence of the lig gene and primary structure of DNA ligase of Escherichia coli.

8. The small RNA GcvB regulates sstT mRNA expression in Escherichia coli.

9. DNA sequence and characterization of GcvA, a LysR family regulatory protein for the Escherichia coli glycine cleavage enzyme system.

10. Penetration of human intestinal epithelial cells by Salmonella: molecular cloning and expression of Salmonella typhi invasion determinants in Escherichia coli.