| Literature DB >> 32227678 |
Jonathan A M Lucas1, James D Hayhurst1, Thomas R Turner1,2, Arthur W Gymer1, Gayle Leen1,2, James Robinson1,2, Steven G E Marsh1,2, Neema P Mayor1,2.
Abstract
We have developed a genotyping assay that produces fully phased, unambiguous HLA-E genotyping using Pacific Biosciences' single molecule real-time DNA sequencing. In total 212 cell lines were genotyped, including the panel of 107 established at the 10th International Histocompatibility Workshop. Our results matched the previously known HLA-E genotype in 94 (44.3%) cell lines, in all cases either improving or equalling previous genotyping resolution. Three (1.4%) cells had discrepant HLA-E genotyping data and 115 (54.2%) had no previous HLA-E data. The HLA-E genotypes for four (1.9%) cell lines resulted in a change of zygosity by identifying two distinct haplotypes. We discovered eight novel HLA-E alleles, extended the known reference sequence of seven and confirmed the existence of a further 10.Entities:
Keywords: HLA; HLA-E; International HLA and Immunogenetics Workshop; SMRT sequencing; cell lines; genetic polymorphism
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Year: 2020 PMID： 32227678 DOI： 10.1111/tan.13882
Source DB: PubMed Journal: HLA ISSN： 2059-2302 Impact factor: 4.513