Masood Abu-Halima1, Zyiad Abu Khaizaran2, Basim M Ayesh3, Ulrike Fischer4, Salem Abu Khaizaran2, Feras Al-Battah5, Mohamad Hammadeh6, Andreas Keller7, Eckart Meese4. 1. Institute of Human Genetics, Saarland University, Homburg/Saar, Germany. Electronic address: masood@daad-alumni.de. 2. Razan Medical Center for IVF and Infertility, Bethlehem, Palestine. 3. Department of Laboratory Medical Sciences, Alaqsa University, Gaza, Palestine. 4. Institute of Human Genetics, Saarland University, Homburg/Saar, Germany. 5. Department of Biology and Biotechnology, Arab American University-Jenin, Jenin, Palestine. 6. Department of Obstetrics and Gynecology, IVF and Andrology Laboratory, Saarland University, Homburg/Saar, Germany. 7. Chair for Clinical Bioinformatics, Saarland University, Saarbruecken, Germany.
Abstract
OBJECTIVE: To identify differentially abundant miRNAs in sperm samples and spent culture media (SCM) of embryos of different grade toward a prediction of pregnancy outcome. DESIGN: Array-based reverse-transcription quantitative polymerase chain reaction profiling and validation. SETTING: University research institute and in vitro fertilization center. PATIENT(S): Couples (n = 61) undergoing infertility treatment with the use of intracytoplasmic sperm injection. INTERVENTIONS(S): None. MAIN OUTCOME MEASURE(S): Abundance levels of miRNAs in combined SCM of embryos of different quality and in sperm samples associated with pregnancy outcome. RESULT(S): Out of 372 screened miRNAs, miR-19b-3p and let-7a-5p were detected consistently in all SCM and sperm samples. The abundance levels of miRNAs were significantly altered between SCM of embryos with different quality (G1, G2, and G3 grades). Specifically, miR-320a and miR-15a-5p were differentially abundant in G1 vs. G2, miR-21-5p in G1 vs. G3, and miR-20a-5p in G2 vs. G3. The abundance levels of combined SCM and sperm derived miRNAs were also significantly altered between different pregnancy outcomes. MiR-19b-3p showed the highest area under the receiver operating characteristic curve values between positive and negative outcomes, with lower abundance levels in both combined SCM and sperm samples associated with a positive pregnancy outcome. MiR-320a, miR-15a-5p, miR-21-5p, and miR-20a-5p showed similar results in combined SCM samples. CONCLUSION(S): miRNA abundance levels in combined SCM and sperm differed significantly depending on embryo quality and pregnancy outcome. MiR-19b-3p may serve as a potential biomarker to predict pregnancy outcome.
OBJECTIVE: To identify differentially abundant miRNAs in sperm samples and spent culture media (SCM) of embryos of different grade toward a prediction of pregnancy outcome. DESIGN: Array-based reverse-transcription quantitative polymerase chain reaction profiling and validation. SETTING: University research institute and in vitro fertilization center. PATIENT(S): Couples (n = 61) undergoing infertility treatment with the use of intracytoplasmic sperm injection. INTERVENTIONS(S): None. MAIN OUTCOME MEASURE(S): Abundance levels of miRNAs in combined SCM of embryos of different quality and in sperm samples associated with pregnancy outcome. RESULT(S): Out of 372 screened miRNAs, miR-19b-3p and let-7a-5p were detected consistently in all SCM and sperm samples. The abundance levels of miRNAs were significantly altered between SCM of embryos with different quality (G1, G2, and G3 grades). Specifically, miR-320a and miR-15a-5p were differentially abundant in G1 vs. G2, miR-21-5p in G1 vs. G3, and miR-20a-5p in G2 vs. G3. The abundance levels of combined SCM and sperm derived miRNAs were also significantly altered between different pregnancy outcomes. MiR-19b-3p showed the highest area under the receiver operating characteristic curve values between positive and negative outcomes, with lower abundance levels in both combined SCM and sperm samples associated with a positive pregnancy outcome. MiR-320a, miR-15a-5p, miR-21-5p, and miR-20a-5p showed similar results in combined SCM samples. CONCLUSION(S): miRNA abundance levels in combined SCM and sperm differed significantly depending on embryo quality and pregnancy outcome. MiR-19b-3p may serve as a potential biomarker to predict pregnancy outcome.
Authors: Gabriela de Freitas Laiber Pascoal; Gabriela Machado Novaes; Monique de Paula Sobrinho; André Bubna Hirayama; Inar Alves Castro; Thomas Prates Ong Journal: Antioxidants (Basel) Date: 2022-04-30
Authors: Angelika V Timofeeva; Ivan S Fedorov; Alexander G Brzhozovskiy; Anna E Bugrova; Vitaliy V Chagovets; Maria V Volochaeva; Natalia L Starodubtseva; Vladimir E Frankevich; Evgeny N Nikolaev; Roman G Shmakov; Gennady T Sukhikh Journal: Diagnostics (Basel) Date: 2021-04-20